SOW_NS1000 Upgrade.pdf
PDF 123 KB Posted
- Attached to
- NextSeq1000 to NextSeq2000 upgrade. Federal contract opportunity
- Solicitation number
- 7571TE26Q00048
About this file
This is a Statement of Work for a NextSeq1000 to NextSeq2000 upgrade service to be performed by Illumina for the National Institutes of Health (NIH), National Eye Institute (NEI). The objective is to upgrade the government's current NextSeq1000 sequencing system to NextSeq2000 capability, which will expand the system's sequencing flexibility by enabling use of all four flow cell types (P1, P2, P3, and P4) rather than the P1 and P2 limitations of the 1000 model. The upgraded system will support sequencing capacities ranging from 100 million to 1.8 billion reads depending on flow cell selection.
The contract is structured as a fixed-price engagement with an estimated performance period of one week. Work will be performed on-site at NIH, Building 6, Room 328. The contractor (Illumina) is responsible for supplying all parts, equipment, and materials required to complete the upgrade, with no Government Furnished Equipment or Information to be provided by NEI. The primary deliverables are all supplies necessary for the upgrade and a fully functional NextSeq2000 instrument. The government will coordinate scheduling with the contractor to identify an appropriate time for the work. No security requirements are associated with this engagement.
View the file
On GovTribe
Work with this file on GovTribe
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
Statement of Work (Illumina)-2025.
1. Delivery Order Title and Scope:
NextSeq1000 to NextSeq2000 upgrade.
This work is intended to convert our current NextSeq1000 sequencing system to a NextSeq2000
2. Background:
The NextSeq 1000 and NextSeq 2000 Sequencing systems are bench top platforms that utilize innovative patterned flow cell technology for diverse sequencing applications. They utilize XLEAP-SBS chemistry, a faster, higher quality, and more robust SBS chemistry built on the proven foundation of standard Illumina SBS chemistry. XLEAP- SBS nucleotides use state-of-the-art dyes and novel linkers and blocks that are more resistant to heat, show a 50× reduction in hydrolysis, and 2.5× faster block cleavage to reduce phasing and prephasing. The XLEAP-SBS polymerase is engineered to incorporate nucleotides faster and with higher fidelity than ever before. There are four different flow cells with varying sequencing capacity that allow for maximum sequencing flexibility.
The P1 flow cell can produce up to 100 million paired reads while the P2 flow cells can generate 400 million reads. The high capacity P3 flow cell can produce up to 1.2 billion reads and the P4 flow cells up to 1.8 billion reads.
In terms of the differences between the 1000 and 2000 systems, the NextSeq 1000 can only use the P1 and P2 flow cells while the NextSeq 2000 sequencer can use all four flowcells. Thus, you can have maximum flexibility with the NextSeq 2000 instrument.
3. Objectives:
a. The goal of this project is to upgrade our NextSeq 1000 machine to a NextSeq2000.
4. Requirements:
a. The Government will:
i. Work with the contractor to schedule an appropriate time for when the work can be done
b. The Contractor will:
i. Perform the upgrade of our current NextSeq 1000 system to NextSeq2000 capability
ii. Will supply all parts and equipment required for the work
5. Contract Type:
a. Fixed price
6. Place of Performance:
i. This work will be done on the campus of NIH, Bldg 6, Rm 328.
7. Period of Performance:
a. This work is estimated to take 1 week to complete.
8. Security:
a. There are no obvious security requirements associated with this request. This
SOW is for services that the contractor (Illumina) will provide. They will travel to NIH for this work.
9. Government Furnished Equipment (GFE)/Government Furnished Information (GFI):
a. We (NEI) will not be providing any equipment in support of this service.
10. Deliverables:
a. The contractor will provide all the supplies needed to complete this upgrade.
b. A fully functional NextSeq 2000 instrument
File details come from the government source that posted it. Updated .