SOW--FMDV Kit.doc

Statement of Work Project: Purchase of FMD Virus RNA Test Kit (FMD with IC wet Assay)

GENERAL INFORMATION

1.0 Scope of Work: Purchase of FMD Virus RNA Test Kit (FMD with IC wet Assay) to perform the real-time PCR to detect FMD Virus RNA. To be used in USDA, APHIS, VS, NVSL.

2.0 Background: The FMD Virus RNA Test Kit (FMD with IC wet Assay) is intended for the rapid in vitro qualitative detection of FMD Virus RNA by real-time polymerase chain reaction (qPCR). The reagents should be used to detect viral RNA extracted from serum and epithelial samples collected from cattle, swine, and sheep. To be used in USDA, APHIS, VS, NVSL.

CONTRACTOR REQUIREMENTS

3.0 Technical Requirements/Tasks:

Minimum Must Have Specifications

· Should have 96 reaction per kit and include master-mix/ enzyme/ positive and negative control

· 10 plate packaging is acceptable

· Ability to store and ship the kits to NVSL as needed or when requested

· Ability to test extracted RNA from epithelium, serum, and oral fluid

· Compatible to use with Thermo Fisher Scientific MagMAX™ Pathogen RNA/DNA Kit and Thermo Fisher AM 1836 extraction kits 4.0 Government Furnished: N/A

5.0 Deliverables/Schedule : Must be delivered within 60 days of date of award or able to store for delivery when requested

6.0 Evaluation Factors Technical capability,

7.0 Technical Point of Contact: Should there be technical questions, please contact Karthik Shanmuganatham , PH: 515-337-7929, email: Karthik.K.Shanmuganatham@usda.gov

8.0 Place of Delivery; USDA APHIS,NVSL, 1920 Dayton Avenue, Ames, IA 50010