SOW Enrichment Media 123A9422Q0035.pdf

US Department of Agriculture (USDA)

Statement of Work for

123A9422Q0035

Contents Project General Information

1.0 Scope of Work

2.0 Background

Contractor Requirements

3.0 Technical Requirements / Tasks

5.0 Deliverables / Schedule

Project Statement of Work of Buffered Peptone Water (BPW), Modified UVM Broth (UVM) and Modified Tryptic Soya Broth (mTSB).

General Information

1.0 Scope of Work

The FSIS (Food Safety and Inspection Service) Laboratories perform regulatory testing of meat, poultry, and egg products for the presence of food borne pathogens such as Salmonella, Listeria monocytogenes, and Shiga toxin producing E. coli.

These test methods require the use primary enrichment media to initiate the growth and recovery of the target bacteria. The FSIS Field Service Laboratories (FSL) use large volumes of enrichment media on a daily basis. The three most used media are being combined into a single solicitation. These media are: Buffered Peptone Water (BPW), Modified UVM Broth (UVM) and Modified Tryptic Soya Broth (mTSB).

2.0 Background

The Food Safety and Inspection Service (FSIS), a public health regulatory agency of the U.S. Department of Agricultural (USDA), protects consumers by ensuring that meat, poultry, Siluriformes fish, and processed egg products are safe, wholesome, and accurately labeled. The FSLs, located in Saint Louis MO, Athens GA, and Albany CA are regulatory laboratories that analyze meat, poultry, Siluriformes fish, and pasteurized egg products to ensure they are free from adulteration.

Contractor Requirements

3.0 Technical Requirements / Tasks

Contract type: requesting an IDIQ (Indefinite Delivery-Indefinite Quantity) with a base year and 4 (four) one-year option years.

The contractor must provide all media in a dehydrated form, powdered, or granulated, and packaged in all options listed below.

BPW:

1. Bulk 10 kilogram (kg) container, and

2. Five (5) 2 kg individual packages in a single container (10 kg total).

UVM:

1. Bulk 10 kg container, and

2. Two (2) 5.2 kg individual packages in a single container (10.4 kg total) mTSB:

1. Bulk 10 kg container, and

2. Three (3) 4.3 kg individual packages in a single container (12.9 kg total)

The following lists each media and their individual components. These formulations may be found in the USDA-FSIS- Microbiology Laboratory Guidebook, Appendix 1, Media and Reagents. Validated alternative formulations are acceptable.

BPW

• Peptone 10.0g

• Sodium Chloride 5.0g

• Sodium Phosphate, dibasic 3.5g

• Potassium Phosphate, monobasic 1.5g

UVM

• Proteose Peptone 5.0g

• Tryptone 5.0g

• Lab Lemco Powder 5.0g

• Yeast Extract 5.0g

• NaCl 20.0g

• KH2PO4 1.35g

• Na2HPO4 12.0g

• Esculin 1.0g

• Naladixic Acid (2% in 0.1 M NaOH) 1.0mL

• Acriflavin 12.0mg mTSB

• Enzymatic digest of casein 17.0g

• Acid digest of casein 10.0g

• Sodium Chloride 5.0g

• Dipotassium Phosphate 4.0g

• Enzymatic digest of Soybean Meal 3.0g

• Dextrose 2.5g

• Bile Salts #3 1.5g

Upon receipt at the laboratory, the media must pass the following criteria:

• Appearance

• pH

• Sterility and growth challenge

5.0 Deliverables / Schedule

The contractor shall provide the following:

• A Safety Data Sheet (SDS) shall be included (or available online) with the first shipment only.

• A certificate of analysis shall be sent (or available online) with each shipment/lot verifying pH, appearance, sterility, inhibitory (if applicable), and recovery testing. Certificates shall list the product name and description, lot or batch number, list of quality control organism(s), shelf life, and signature and dates of responsible person(s).

• There shall be a minimum 1-year shelf life from date of receipt.

• All shipping charges are included in the price of the product.

Delivery Address

The contractor to deliver to the following addresses:

USDA, FSIS, OPHS, Midwestern Laboratory Federal Center Building 105D 4300 Goodfellow Blvd Saint Louis, MO 63120

USDA, FSIS, OPHS, Eastern Laboratory 950 College Station Rd Athens, GA 30605

USDA, FSIS, OPHS, Western Laboratory

WRRC, ARS, WAB

800 Buchanan St.

Albany, CA 94710