Sources Sought Attachment.docx

DRAFT

PRICING SCHEDULE

Model : 480 (Brand Name or Equal)

Model : 480 (Brand Name or Equal)

Model : 480 (Brand Name or Equal)

Model : 480 (Brand Name or Equal)

Model : 780 (Brand Name or Equal)

Installation

Each

TOTAL (Items 0001 – 0006)

Statement of Work

Project: Replacement of Liquid Handling Platforms at NBAF/FADDL

GENERAL INFORMATION

1.0 Scope of Work

The goal of this project is to acquire and implement widescale robotics for supporting diagnostic testing and outbreak surge capacity at the Foreign Animal Disease Diagnostic Laboratory (FADDL). This will occur in multiple sections at the National Bio and Agro Defense Facility (NBAF) in Manhattan, KS.

2.0 Background

The purpose of the procurement of automatic robotics machines is to replace the existing EVO systems at NBAF with more appropriate automation systems. These instruments will perform the following activities: Sample preparation (aliquoting from submitter tubes to 96-well plates, decapping and recapping various sized cryovials, sealing plates), PCR mastermix preparation (using kit based PCR reagents to set up PCR plates with mastermix), serological assays (ELISA kit-based assays including plate washing, incubations and moving submitted samples from tube to plates) and sequencing preparation (library preparation, amplification on a conventional thermal cycler, small volume manipulation etc) for USDA Veterinary Services (VS), National Veterinary Services Laboratories (NVSL), NBAF, FADDL.

· Capable of processing 96 samples at one time

· Require tube sizes of 2ml, 5ml and 12ml cryovials and 8.5ml serum separator tubes

· Required operations

· 8 channel pipette arm (Span-8 equivalent)

· Plate/tube manipulation arm

· Scanning the barcodes for each of the 96 tubes (2D scanning)

· Decapping the tubes

· Pipetting a volume of 50-500uL of fluid from the tubes to a standard PCR plate or deep well plate

· Recapping the tubes

· Sealing 96-well or deep well plate

· Minimum of three 320ml reagent reservoirs

· Ability to move tubes and plates across worktable

· Heating/Cold block functions on one site for a 96-well plate

· Thermoshake device

· All operations must be provided in a fully enclose, HEPA filtered cabinet (Negative Flow)

· Weight less than 200lbs

· Power Requirements: 120V/50-60 Hz

· Must be smaller than 46 inches width or depth

· Machine must be situated on an included cabinet (not benchtop)

3.2 Machine 2: Tecan Fluent w/air FCA & RGA-FES & Negative Flow HEPA Hood (Virus Isolation), model 480 (brand name or equal) The following features/salient characteristics are required:

· Capable of processing 96 samples at one time

· Require tube sizes of 2ml, 5ml and 12ml cryovials and 8.5ml serum separator tubes

· Required operations

· 8 channel pipette arm (Span-8 equivalent)

· Plate/tube manipulation arm

· Scanning the barcodes for each of the 96 tubes (2D scanning)

· Decapping the tubes

· Pipetting a volume of 50-500uL of fluid from the tubes to a standard PCR plate or deep well plate

· Recapping the tubes

· Sealing 96-well or deep well plate

· Minimum of three 320ml reagent reservoirs

· Ability to move tubes and plates across worktable

· Heating/Cold block functions on one site for a 96-well plate

· Thermoshake device

· All operations must be provided in a fully enclose, HEPA filtered cabinet (Negative Flow)

· Weight less than 200lbs

· Power Requirements: 120V/50-60 Hz

· Must be smaller than 46 inches width or depth

· Machine must be situated on an included cabinet (not benchtop) Machine 3: Tecan Fluent w/air FCA and RGA-FES (MasterMix/Clean Reagent Prep), model 480 (brand name or equal) The following features/salient characteristics are required:

· Capable of processing 96 samples at one time

· Require tube sizes of 2ml, 5ml and 12ml cryovials and 8.5ml serum separator tubes

· Required operations

· 8 channel pipette arm (Span-8 equivalent)

· Plate/tube manipulation arm

· Scanning the barcodes for each of the 96 tubes (2D scanning)

· Decapping the tubes

· Pipetting a volume of 50-500uL of fluid from the tubes to a standard PCR plate or deep well plate

· Recapping the tubes

· Sealing 96-well or deep well plate

· Minimum of three 320ml reagent reservoirs

· Ability to move tubes and plates across worktable

· Heating/Cold block functions on one site for a 96-well plate

· Thermoshake device

· Weight less than 200lbs

· Power Requirements: 120V/50-60 Hz

· Must be smaller than 46 inches width or depth

Machine 4: Tecan Fluent w/air FCA & RGA-FES & Negative Flow HEPA Hood (Serology), model 480 (brand name or equal) The following features/salient characteristics are required:

· Capable of processing 96 samples at one time

· Require tube sizes of 2ml, 5ml and 12ml cryovials and 8.5ml serum separator tubes

· Required operations

· 8 channel pipette arm (Span-8 equivalent)

· Plate/tube manipulation arm

· Scanning the barcodes for each of the 96 tubes (2D scanning)

· Pipetting a volume of 5-1000uL of fluid from the tubes to a standard PCR plate or deep well plate

· Sealing 96-well or deep well plate

· Minimum of three 320ml reagent reservoirs

· Ability to move plates across worktable

· Cold block functions on one site for a 96-well plate

· Capable of incubating up to six 96-well plates at temps ranging from 25°C to 37°C.

· Incubator (6-position)

· Plate washer system

· All operations must be provided in a fully enclose, HEPA filtered cabinet

· Weight less than 200lbs

· Power Requirements: 120V/50-60 Hz

· Must be smaller than 46 inches in any dimension

· Machine must be situated on an included cabinet (not benchtop) Machine 5: Tecan Fluent NGS DreamPrep 2.0 MCA 96 with Infinite M Nano+Reader and Negative Flow HEPA Hood (NGS Library prep/Sequencing support), model 780 (brand name or equal) The following features/salient characteristics are required:

· Capable of processing 96 samples at one time

· Require tube sizes of 2ml, 5ml and 12ml cryovials and 8.5ml serum separator tubes

· Required operations 8 channel pipette arm (Span-8 equivalent)

· Plate/tube manipulation arm

· 96-position pipetting arm (MCA equivalent)

· Scanning the barcodes for each of the 96 tubes (2D scanning)

· Pipetting a volume of 5-1000uL of fluid from the tubes to a standard PCR plate or deep well plate

· Sealing 96-well or deep well plate

· Minimum of three 320ml reagent reservoirs

· Ability to move plates across worktable

· Cold block functions on one site for a 96-well plate

· Conventional thermal cycler capable of running 2 and 3 cycle PCRs

· Capable of incubating up to six 96-well plates at temps ranging from 25°C to 37°C.

· Fluorometry plate reader (Integrated)

· All operations must be provided in a fully enclose, HEPA filtered cabinet

· Weight less than 200lbs

· Power Requirements: 120V/50-60 Hz

· Must be smaller than 65” (width or depth)

· Machine must be situated on an included cabinet (not benchtop)

4.0 Deliverables/Schedule: All machines specified must be delivered within 90 days of award. Delivery and installation must be coordinated with the technical point of contact at least 14 days in advance. The contractor shall adapt scheduling as necessary during this period based on the readiness of the Government’s facility and availability of Government personnel.

5.0 Installation : The contractor shall install equipment at time of delivery. The contractor shall schedule delivery and installation with the Government Technical Point of Contact or designee at least two (2) weeks in advance. The contractor must provide all necessary and proper equipment for unloading the equipment, transporting it to the laboratory, and installing the equipment. The contractor shall demonstrate the equipment is functioning according to the manufacturer’s specification upon completion of the installation. A user manual shall be provided at the time of installation.

6.0 Security: The contractor shall comply with all established security policies at (insert name of facility) and shall be escorted and accompanied at all times while at the facility. The contractor shall complete required paperwork for access to the facility.

7.0 Place of Delivery: USDA APHIS, NVSL, 1525 Discovery Way, Manhattan, KS 66502