SOR Instrument Rapid Cell Imager final.docx

STATEMENT OF REQUIREMENTS

TITLE: Rapid Cell Imager

I. BACKGROUND INFORMATION

Biosystems and Biomaterials Division is involved in the development of cell characterization methods for biotechnology that support cell-based assays and the development and manufacturing of cell-based products. This work involves the measurement of cell count, identity, and function. To support the development of reference methods for bioassays, cell characterization, and cell engineering, the division requires capabilities for rapid, whole well imaging of cells grown in a range of culture vessels. Imaging capabilities include label free transmitted light microscopy as well as fluorescent microscopy for labeled cells. Rapid imaging capabilities are required for time sensitive live cell imaging, as well as high throughput analysis of cell samples. Whole-well imaging capabilities are required to support the development of reference methods as well as for capturing rare events within the culture environment.

II. SPECIFICATIONS

The Contractor shall provide one (1) Celigo instrument that meets the following technical requirements.

System Requirements:

· The instrument shall be a benchtop image-based cell analyzer.

· The instrument shall be able to perform imaging in brightfield

· The instrument shall be able to perform imaging in fluorescence in the following channels

· Excitation 377/50 and Emission 470/22 (Blue) to support imaging with dyes including but not limited to Hoechst and DAPI.

· Excitation 483/32 and Emission 536/40 (Green) to support imaging with dyes including but not limited to FITC, Calcein, GFP, AlexaFluor® 488.

· Excitation 531/40 and Emission 629/53 (Red) to support imaging with dyes including but not limited to R-PE, PI, Texas Red, AlexaFluor® 568.

· Excitation 628/40 and Emission 688/31 (Far-Red) to support imaging with dyes including but not limited to DRAQ5®, AlexaFluor®647.

· The instrument shall be able to perform high-speed imaging of well plates of at least as fast as 2 minutes per 384-well plate.

· The instrument shall be capable of capturing with spatial resolution between 1 µm/pixel to 8 µm/pixel resolution.

· The instrument shall be capable of performing both partial and whole well imaging for cells, colonies, plaques, spheroids.

· Instrument controls shall be compatible with integration within a Hamilton liquid handing automation system.

· Instrument shall be provided with accompanying software with capabilities for

· analyzing brightfield cell-based assays including cell counting, confluence measurements, growth curves, proliferation, cell morphology analysis, cytopathic effects, colony formation, colony growth monitoring, 3-D spheroid analysis, and cell migration.

· Analyzing fluorescent cell-based assays including cell health, cell viability, apoptosis, DNA synthesis/proliferation, cell cycle, fluorescent marker analysis, transfection/transduction efficiencies, multi-color fluorescent colony analysis, and 3D spheroids fluorescent analysis.

· Instrument shall be provided with copies of user operation manuals in either electronic or hard-copy format.

Installation:

The Contractor shall install the instrument in Building 224, at NIST Gaithersburg, MD. Installation shall include, at a minimum, uncrating/unpacking of all equipment, set-up and hook-up of all equipment, start-up, demonstration of specifications, and removal of all trash. Instrument will operate to manufacturer’s specifications upon installation. Installation will take place during normal business hours, between 8:30 am and 5:00 pm Eastern Time, Monday through Friday except Federal Holidays, and will be coordinated with the NIST Contract Officer Representative (COR) and the Technical Subject Matter Expert (SME).

Installation is to occur within 30 calendar days of delivery.

Warranty:

The Contractor shall provide, at a minimum, a one-year warranty for the instrument. The warranty shall cover all parts, labor and travel. The warranty shall commence upon Government acceptance.

Training:

The contractor shall provide training at NIST Gaithersburg for up to 5 NIST personnel covering normal operation, troubleshooting, and routine maintenance. Training will be provided during normal business hours, between 8:30am and 5:00pm Eastern Time, Monday through Friday, except Federal Holidays, and will be coordinated with the NIST Contract Officer Representative (COR) and the Technical Subject Matter Expert (SME) to ensure maximum availability of NIST personnel.

Training is to occur within 10 business days of installation. Training may be completed on-site at NIST immediately after installation and demonstration of specifications.

Options for Service/Maintenance After Initial Warranty The Contractor shall provide two (2) options for annual service in 12-month periods beginning at the conclusion of the included one-year warranty. Service options shall include one (1) preventative maintenance visit annually and unlimited repair visits on-site at NIST. Service shall cover all parts, labor and travel for preventative maintenance visits and service visits.

Service Report

The contractor shall submit a service report prior to departure from NIST on any given service call for maintenance and repairs. The service report shall include the following:

1. Model number.

2. Serial number.

3. Date and time of arrival to NIST.

4. Description of service performed.

5. Name and quantity of any parts that were repaired.

6. Name and quantity of any parts that were replaced.

7. Date and time of departure from NIST.

8. Diagnostic test results or narrative summary demonstrating that the instrument is meeting the performance standards.

9. Any additional comments or recommendations.

10. Printed name and signature of service technician. Signature block for COR accepting the services rendered

III. DELIVERY

Delivery shall be F.O.B Destination and shall occur within 120 calendar days of award.

F.O.B. Destination means: The contractor shall pack and mark the shipment in conformance with carrier requirements, deliver the shipment in good order and condition to the point of delivery specified in the purchase order, be responsible for any loss of and/or damage to the goods occurring before receipt and acceptance of the shipment by the consignee at the delivery point specified in the order, and pay all charges to the specified point of delivery.

Delivery shall occur during normal business hours, between 8:30 am and 5:00 pm Eastern time, Monday through Friday, with the exception of Federal holidays and shall be made to:

National Institute of Standards and Technology 100 Bureau Drive Gaithersburg, MD 20899 ATTN: Sumona Sarkar 224/A109

IV. DELIVERABLES

Description (and Format)

Celigo Instrument (Ship to NIST)

Software (Ship to NIST or electronic download) One (1) set

NIST

COR

120 calendar days after award

Operations and maintenance manual for the instrument (Hardcopy or electronic) One (1) set

Installation (On-site at NIST)

Training of NIST personnel at NIST, Gaithersburg, MD (On-site at NIST)

V. ACCEPTANCE CRITERIA

Formal acceptance of the system shall be given at the time the Contractor successfully demonstrates that all performance specifications in this Statement of Requirements have been met including installation and training. The NIST COR shall review all deliverables and determine if these criteria are met.

For maintenance and/or repair services performed during optional service agreement years, Contractor shall demonstrate that the instrument is operating to OEM specifications and document such operation on the service ticket after maintenance and/or repair. The NIST COR shall review the service ticket to determine if these criteria are met.

VI. GENERAL INFORMATION

Safety: The Contractor employee shall be responsible for knowing and complying with site safety regulations. Such regulations include, but are not limited to, general safety, fire prevention, and waste disposal.

Security: NIST is a restricted campus. An identification badge is required for access for entry into buildings and also is shown to the armed Security Police when entering the campus.

Identification Badges: Contractor employees shall comply with NIST identification and access requirements. The Contractor shall provide the name and citizenship information of employees for on-site visits to the NIST COR and/or SME prior to arrival. The COR and/or SME will submit the name(s) and citizenship information to the NIST Office of Security in order for a given Contractor employee to enter the NIST campus. The Contractor employee is responsible for absences due to missing or expired identification and access documents. Each Contractor employee shall wear a visible identification badge provided by the NIST Security Office.

Vehicle Registration: All Contractor employees must register their vehicles with the NIST Security Office to gain access to the campus. A valid driver’s license, Government-furnished, REAL ID compliant civilian ID (which in most cases can be a valid driver's license), proof of insurance and current registration must be presented to the NIST Security Office.