soliciation.pdf

SOLICITATION

(1) Action Code: Solicitation

(2) Date: 02/09/2021

(3) Year: 2021

(4) Contracting Office Zip Code: 20892

(5) Classification Code: Q301

(6) Contracting Office Address:

6705 Rockledge Drive Rockledge I Bethesda, MD 20892

(7) Subject/Title: Single cell RNA sequencing (scRNA-seq) services

(8) Proposed Solicitation Number: 75N92021Q0047

(9) Closing Response Date: 02/15/2021

(10) Contact Point(s): Elizabeth Francois, Contract Specialist

(11) Contract Award and Solicitation Number: TBD

(12) Contract Award Dollar Amount: TBD

(13) Contract Line Item Number(s): TBD

(14) Contractor Award Date: TBD

(15) Contractor Name: The Institute for Molecular Medicine, Finland (FIMM)

(16) Description:

(i) This is a solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued.

(ii) The solicitation number is 75N92021Q0047 and the solicitation is issued as a request for quotation (RFQ).

(iii) The solicitation document, the incorporated provisions, and clauses are those in effect through Federal Acquisition Circular 2021-04 effective 01/19/2021.

(iv) This solicitation is not set aside. The associated NAICS code is 611310 and the small business size standard is $30.0M. This acquisition is being conducted in accordance with the procedures of FAR Part 12 and FAR Part 13.

(v) It is anticipated that there will be one (1) contract line item listing all components.

The specifications and quantities of required items are listed below.

(vi) The requested requirements are listed in the attached Statement of Work.

(vii) This is a sole source requirement to Institute for Molecular Medicine, Finland (FIMM) for Single cell RNA sequencing (scRNA-seq) services. A Notice of intent to solicit and award for sole source was posted on 02/03/2021.

(viii) Period of Performance:

Within 60-90 days after receipt of order

(ix) The provision at FAR clause 52.212-1, Instructions to Offerors – Commercial Items, applies to this acquisition.

• The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers:

o Offeror can provide all required services/deliverables as described in the Statement of Work.

o Quotes should include all related pricing and cost including shipping and handling.

(x) FAR clause 52.212-2, Evaluation – Commercial Items is applicable to this acquisition.

(xi) Please include a completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications – Commercial Items, with all offers. An offeror shall complete only paragraphs (b) of this provision if the offeror has completed the annual representations and certificates electronically via http://www.acquisition.gov. If an offeror has not completed the annual representations and certifications electronically at the System for Award Management (SAM) website, the offeror shall complete only paragraphs (c) through (p) of this provision.

(xii) FAR clause at 52.212-4, Contract Terms and Conditions – Commercial Items, applies to this acquisition.

(xiii) FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders – Commercial Items, applies to this acquisition.

• 52.204-25, Prohibition on Contracting for Certain Telecommunications and Video Surveillance Services or Equipment. (AUG 2019) (Section 889(a)(1)(A) of Pub. L. 115-232).

• 52.217-9, Option to Extend the Term of the Contract (Mar 2000)

• 52.222-3, Convict Labor (June 2003) (E.O. 11755)

• 52.222-21, Prohibition of Segregated Facilities (APR 2015)

• 52.222-26, Equal Opportunity (APR 2015) (E.O. 11246)

• 52.222-36, Equal Opportunity for Workers with Disabilities (JUL

2014) (29 U.S.C. 793)

• 52.222-50, Combating Trafficking in Persons (MAR 2015). (22

U.S.C. chapter 78 and E.O. 13627)

• 52.222-53, Exemption from Application of the Service Contract

Labor Standards to Contracts for Certain Services- Requirements

• 52.223-18, Encouraging Contractor Policies to Ban Text

Messaging While Driving (AUG 2011) (E.O. 13513)

• 52.225-13, Restrictions on Certain Foreign Purchases (JUN 2008)

• 52.232-33, Payment by Electronic Funds Transfer—System for

Award Management (JUL 2013) (31 U.S.C. 3332)

(xiv) Additional Contract Requirements: None

(xiv) The Defense Priorities and Allocations System (DPAS) and assigned rating are not applicable.

(xv) Offers shall be submitted via the FedConnect web portal (www.fedconnect.net). The due date for receipt of offers is 12:00 PM Eastern Time on 02/15/2021.

(xvi) Point of Contact:

Elizabeth Francois, Contract Specialist www.fedconnect.net solicitation 75N92021Q0047

(17) Place of Contract Performance: N/A

(18) Set-aside Status: None http://www.fedconnect.net/ http://www.fedconnect.net/

STATEMENT OF WORK

Project: Single cell RNA Sequencing (scRNA-seq) services for eight (8) samples

Background:

The National Institute of Dental and Craniofacial Research (NIDCR) is a branch of the U.S. National Institutes of Health (NIH). The institute aims to improve dental, oral, and craniofacial health through research, research training, and the dissemination of health information. The overall research goal of Dr.

Kerosuo’s lab is to understand how stemness is initiated and maintained in the neural crest, which is a stem cell population found in all vertebrate embryos at very early stages of development. The neural crest gives rise to components of multiple organs, the peripheral nervous system, craniofacial skeleton, and pigmentation to name a few. The requested single cell RNA sequencing (scRNA-seq) analysis is a part of an ongoing project where the Kerosuo’s lab investigators analyze stemness formation in chicken embryos by using multiple sophisticated techniques including highly multiplex spatial single cell transcriptomics, which determines gene expression of predetermined candidates with high special resolution, as well as bulk-RNAseq that reveals novel players. The scRNA-seq data also provides an opportunity to perform pseudo time analysis to understand linearity of cell fate determination in the neural crest. This additional piece of data will perfectly complement these two techniques by revealing transcriptional profiles including unknown novel genes on a single-cell level, and increase our chances of a high impact publication.

Purpose:

Single cell RNA sequencing (scRNA-seq) is a method used to identify transcriptionally distinct subpopulations within a given tissue. Dr. Kerosuo’s lab studies neural crest development, and this collaborative project aims to understand transcriptional profiles of neural crest subpopulations during various stages of development. This is a high quality method and the Kerosuo lab investigators expect the results to reveal interesting details on neural crest development, which will be an essential part of data in their ongoing investigation that will be submitted for publication in a high impact journal within the year 2021. The results will also be publicly available for the neural crest research field and thus serve a large community working on craniofacial development, which is in line with the central mission of

NIDCR.

Contractor Requirements (Scope of Work):

The contractor must be a local company near the University of Helsinki, Meilahti research campus in the Northern Finland area. The cells for these samples need to be collected fresh and there is a maximum two-hour window from when the cells are collected to when they need to be processed. Since there are eight samples, the dissection process itself takes two hours (when first samples have been waiting for two hours the last samples are being dissected out).

The contractor shall provide the following services:

• Cell count and viability measurement

• Single cell partitioning using 10X Genomics Chromium Controller

• Capture of 500 – 10,000 cells per sample prep

• Reverse transcription and barcoding, cDNA amplification and quality check

• Library preparation and library quality check

• Library quantification (Q-PCR) and next generation sequencing (Illumina NovaSeq 6000 system, FIMM Genomics Sequencing Unit)

• Raw base call (BCL) file demultiplexing and fastq file generation (Cell Ranger mkfastq pipeline, 10x Genomics)

• Sample specific single cell gene expression analysis and data aggregation (Cell Ranger count and aggr pipelines, 10x Genomics)

Deliverables:

a. Raw sequence (fastq) files

b. Single cell 3' gene expression data generated with Cell Ranger count pipeline (10x Genomics)

c. Combined data from multiple samples in an experiment-wide gene-barcode matrix and analysis generated with the Cell Ranger aggr pipeline (10X Genomics)

Period of Performance:

The eight samples will be delivered to the contractor between March 1st and July 31st 2021.