SOLE SOURCE JA_For Contract File_Kim DNA Extractor MSK_Redacted.pdf

SOLE SOURCE or BRAND NAME JUSTIFICATION AND APPROVAL FOR

PURCHASES NOT EXCEEDING THE SIMPLIFIED ACQUISITION THESHOLD

1. DESCRIPTION OF THE SUPPLIES/SERVICES REQUIRED

BIL projects ERR09 and ERR10 require a DNA extractor for a five-year, research projects developing the tools of detecting invasive forest pathogens in US borders and tree nurseries. The projects involve domestic and international nurseries/gardens/arboretums that span diverse climates and woody plants. Reliable and high-quality DNA from various biological samples (e.g., fungi, plants, spores, water, soil, etc.) is essential to the success of the projects. The projects requires a one (1) DNA purification system.

Requesting organization: USDA Forest Service – Pacific Northwest Research Station

Items: KingFisher™ Duo Prime Purification System with SmartStart

Total Estimated Value:

2. JUSTIFICATION FOR SOLICITING FROM A SINGLE SOURCE or FOR A BRAND

NAME

Single Source Justification:

Fisher ScientificTM (ThemoFisher Scientific) is the sole manufacturer and distributor of the following equipment needed for this project:

Thermo Scientific™ KingFisher™ Duo Prime Purification System with SmartStart: Our BIL ERR09/ERR10 projects face to serious challenge for extracting DNA from forest soil samples.

We tried to use our conventional DNA extractor (tissue disruptor) for extracting DNA from these soil samples. Unfortunately, this conventional tissue disruptor resulted in no DNA or very poor yield and quality of DNA which are not usable for our purpose. It is essential to produce high quality and high yield DNA, RNA and proteins from a variety of starting samples for our BIL projects. The Duo Prime Purification System with SmartStart from ThermoFisher Scientific is providing a unique magnetic bead technology which allows the separation of DNA from crude samples such water, cultivated media, soil, fungal spores. Further, this DNA purification system can process many samples (e.g., 96 samples at a time) with automation capacity. The item being purchased will be placed at the Forest Pathology Laboratory – Corvallis Forestry Sciences Lab where the various biological samples need to be processed for the BIL projects. Due to delayed purchase of this DNA purification system, the entire BIL projects are jeopardizing to meet its deadline. High quality and high yield DNA (and later RNA and proteins) are prerequisite for proceeding our BIL projects in the next steps. We know for sure that the Duo Prime Purification System from ThermoFisher Scientific will allow us to proceed our projects to next steps – our counterpart lab (Rocky Mountain Research Station) is currently using this system to successfully process their soil (and other biological) samples. This procurement will allow PNW Research Station to meet the current BIL projects’ requirements.

The product fulfills the following "requirements" for extracting high quality DNA from various biological samples of the study projects:

• Automated magnetic bead technology

• Built-in UV lamp that delivers easy and effective decontamination

• Optional bar-code reader that allows users to track samples directly in internal software

• Software with protocols that can be modified or developed, as well as impo1ted directly to the instrument using PC or via USB stick

• Ability to save nm log files individually, providing u·aceability and complete data management

• Compact footprint size

• Volume (Meu·ic) processing requirement: 96 DW plate 50 to 1000 µL, 24 DW plate 200 to

5000 µL, Elution su-ip 30 to 130 µL

• Paiticle size (Meu-ic) requirement: ca. > 1 µm

To our knowledge, Fisher Scientific™ (ThemoFisher Scientific) is only manufacturer to produce a DNA extractor that uses an automated magnetic bead technology.

3. EFFORTS TO OBTAIN COMPETITION

Alternative suppliers/vendors with similai· features were carefully considered; however, key considerations led us to conclude that Fisher Scientific™ (ThemoFisher Scientific) product remain the optimal choice for our BIL projects.

Reliable to produce high quality DNA from the most recalciu·ant biological samples: While there ai·e a couple of DNA extI·actors available to produce high quality DNA from biological samples, these two DNA exu-actors failed to produce the minimum quality of DNA for our projects. These ai·e the most used DNA extractors for producing DNA from vai-ious staiting materials.

DemonstI·ated system with success: Our collaborator's lab routinely produces high quality DNA from forest soil samples (ve1y similar to our soil samples which ai·e highly recalcitI·ant nature) using Fisher Scientific TM (ThemoFisher Scientific) product.

4. CONTRACTING OFFICER'S DETERMINATION

I have dete1mined, in accordance with FAR 8.405-6(1)(B) that the circumstances of this contI·act action deem only a single source is available for the required services.

Requesting Office: USDA Forest Service Pacific No1thwest Research Station

Requestor:

Title: Research Plant Pathologist

Concur/Non-Concur

Dr. Jerome Watkins

ContI·acting Officer

JeromeWatkinsJr Highlight