Sol_140L0125Q0013.pdf

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Washington DC 20003 20 M Street SE

BLM ACQ & FA (WO855)

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541380

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NORTH AMERICAN INDUSTRY

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07/23/2025 1400 ED

07/15/2025

3032369452Andrew Alger (No collect calls)

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140L0125Q0013

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8 2 of

Request for Information (RFI) Genetic Analysis of Wild Horse and Burro Hair Follicle Samples

Bureau of Land Management (BLM)

This Request for Information/Sources Sought is being published for market research purposes only to identify potential sources capable of supporting the Department of the Interior (DOI), Bureau of Land Management (BLM) for genetic analysis of wild horse and burro hair follicle samples. This is only a Sources Sought Synopsis and is not a Request for Quote (RFQ). A solicitation is not being issued at this time and this notice shall not be construed as a commitment by the Government to issue a solicitation or ultimately award a contract, nor does it restrict the Government to a particular acquisition approach.

Background The BLM Wild Horse and Burro (WH&B) Program Office requires genetic analysis services of wild horse and burro hair follicle samples. The complete Statement of Work (SOW) is attached with this RFI.

Objectives The objective is to establish a contract that provides analysis of follicle samples collected by the BLM and sent to the contractor. More specific details are included in the attached SOW.

Responses to this sources sought synopsis will be used for a market survey analysis to determine interested parties and if the contemplated acquisition will be conducted under full and open competition, total small business set-aside or 8(a) procedures. Respondents shall indicate in their responses whether they qualify under NAICS Code 541380, Testing Laboratories and Services with a size standard of $19M for small business concerns. If you are a small business, and if the Government were to set this acquisition aside for small business, you would be required to comply with Federal Acquisition Regulation 52.219-14, Limitations on Subcontracting. The small business prime contractor must perform at least 50% of the requirement.

RFI Response Responses to this RFI must include the below company details and answers to the following questions. The information supplied in this questionnaire will allow the BLM to better finalize the acquisition strategy plan for a possible resultant procurement.

1. Company Name:

Street Address:

City/State/|Zip:

Point of Contact:

Phone:

Email:

Company Website:

UEI Number:

2. Company currently registered and active in the System for Award Management (SAM)?

☐ YES ☐ NO

3. What is your business size category for NAICS 541380?

☐ SMALL ☐ LARGE ☐ NON-PROFIT

4. If response above is SMALL, please select any of the socio-economic groups that apply to your company:

☐ 8a ☐ HubZone ☐ EDWOSB ☐ SDB ☐ SDVOSB ☐ VOSB ☐ WOSB

5. Provide your qualifications to provide genetic analyses as detailed in attached specifications.

6. Provide your company’s level of certification from the International Society for Animal

Genetics (ISAG). This certification must be at a high enough level to distinguish horse breeds, or be able to produce evidence that error rates for microsatellite genotyping are comparable to or better than those required for that type of ISAG certification.

7. After reviewing of the attached specifications do you have any questions/comments?

8. Would your company be interested in submitting a competitive quote for these services in the future?

☐ YES ☐ NO

9. Please provide any other applicable comments or recommendations not addressed in the foregoing.

Submissions Any information submitted by the respondents to this source sought synopsis is strictly voluntary. If additional pages are needed response should not exceed ten (10) pages.

Only electronic responses (electronic mail) will be accepted. Response shall be received no later than 2:00 P.M. EST, Wednesday, July 23, 2025. Point of Contact for responses, questions and/or comments is Andrew Alger, Contracting Officer, aalger@blm.gov, 303-236-9452.

NOTE: The attached SOW is a "DRAFT" version. If a solicitation is issued vendors will be responsible to review “FINAL” specifications prior to quote submission. It is the offerors' responsibility to ensure any revisions are understood. Additional information, as it becomes available, will be posted as well as amendments to this announcement and will be available for viewing on SAM.gov. It is the responsibility of the interested parties to review the sites frequently for any updates/amendments to documents. All potential offerors are reminded, in accordance with Federal Acquisition Regulations (FAR) 52.204-7 -- System for Award mailto:aalger@blm.gov

Management (SAM) registration is required prior to award of any contract. Lack of registration in SAM will make an offeror ineligible for contract award.

Disclaimer Any information provided by industry to the Government as a result of this sources sought synopsis is strictly voluntary. Responses will not be returned. No entitlements to payment of direct or indirect cost or charges to the Government will arise as a result of contractor submission of responses or the Government's use of such information. The information obtained from industry responses to this notice may be used in the development of an acquisition strategy and future RFQ.

All information received in response to this RFI that is marked proprietary will be handled accordingly.

Draft Statement of Work Genetic Analysis of Wild Horse and Burro Hair Follicle Samples

Background

The Bureau of Land Management (BLM) protects and manages wild horses and burros (WHB) under the authority of the Wild Free-Roaming Horses and Burros Act of 1971 to ensure that healthy herds thrive on healthy rangelands. The BLM monitors herds of free-roaming WHB on public lands. As part of that monitoring activity, BLM’s WHB Program monitors elements of genetic diversity in managed herds.

This monitoring relies on genetic analysis of DNA from sets of samples of hair follicles taken from WHB that have been gathered from the wild. For the genetic analysis to be useful to BLM, it should be consistent with previous analyses. Specific measures and aspects of analysis for sampled herds are detailed in the synopsis specifications.

The BLM Wild Horse and Burro Program seeks to procure services that include the genotyping and analysis of hair follicle samples collected as a routine part of wild horse and burro genetic monitoring.

Here, the determination of the allele lengths for an individual horse’s (or burro’s) hair, at a number of identified microsatellite loci (and, potentially, at other loci), is termed “genotyping.” The analysis sought is to characterize a number of attributes from each set of samples collected, including: observed heterozygosity, similarity to domestic horse (or burro) breed types, similarity to other BLM-managed herds, inbreeding coefficient, number of alleles identified per locus, and presence of any alleles not found in other feral horse samples. BLM is seeking sources that can both conduct the genotyping and produce the analyses for each set of genetic samples. The minimum purchase quantity will be 300 units of individual sample genotyping and 10 analyses. Exact quantities for the number of genetic samples that will be sent for genotyping, or for the number of analyses, are not yet determined, so the ‘indefinite delivery, indefinite quantity’ type of contract is appropriate.

Specifications/Performance Work Statement

BLM sends the contractor samples from individual horses (or burros), in the form of hair and hair follicles that have been pulled from the horse (or burro) mane. Approximately 40 hairs per individual horse or burro are sent, with hair samples from a single individual enclosed in a dry envelope. Samples are labeled by BLM, and samples and explanatory data forms are sent to the contractor. BLM sends samples along with a data sheet that identifies sampled individual horses (or burros) by sex. Sets of samples usually include 30 samples per area being monitored.

The genotyping must be done in such a way that provides reliable characterization of microsatellite allele length for both copies of each sampled individual’s DNA. Currently accepted methods include but are not necessarily limited to using an automated DNA sequencer to separate polymerase chain reaction (PCR) products. Each individual’s DNA must be genotyped at, at the least, 12 microsatellite loci. Data about each individual’s genotype must be stored in a database, and those results must be shared with and periodically sent to BLM.

Qualifications:

The genotyping laboratory must have certification from the International Society for Animal Genetics (ISAG) at a high enough level to distinguish horse breeds, or be able to produce evidence that error rates for microsatellite genotyping are comparable to or better than those required for that type of ISAG certification.

Requirements:

I. Genotyping

The contractor shall:

a) Genotype each individual horse’s (or burro’s) DNA (hair follicle) sample at enough loci to be able to characterize the sample set for analysis. The loci to be used in genotyping must include, but do not need to be limited to, the 12 microsatellite loci used to create the matrix of Fst values in Appendix F of the National Academies of Sciences 2013 report, “Using science to improve the BLM wild horse and burro program.” These loci include VHL20, HTG4, AHT4, HMS7, AHT5, HMS6, ASB2, HTG10, HMS3, ASB17, ASB23, and LEX33. Additional loci that the contractor may choose to genotype are optional;

b) Conduct genotyping with error rates for genotyping at a low enough level that allows for the contractor’s continuing accreditation by the International Society for Animal Genetics (ISAG);

c) In the event that a sample does not amplify clearly enough to distinguish a homozygote from a case of allelic dropout, the laboratory must have procedures in place to repeat the sample genotyping until a reliable result is obtained for that sample (or until it can be concluded that the DNA in the sample is of inadequate quality for amplification).

II. Data transfers and the database

a) For each sample, both copies of length polymorphisms at each locus shall be recorded in a database in such a way that meets ISAG standardization (i.e., so that the allele length labeled “M” is for the polymorphism that ISAG has identified as being in the middle of an identified range of polymorphic lengths for horse [or burro]);

b) The database shall contain records of all genotyped samples, where each sample is also associated with the animal’s identification number provided by BLM on the initial data sheet, sex, geographic source (including state, and herd management area), and date of sample collection (i.e., month and year);

c) The database shall be made using some form of database or spreadsheet computer software that is commonly available and widely used in contemporary American society;

d) The contractor shall send BLM a digital copy of the database with the results of BLM samples’ genotypes and all associated data at least once per every five analyses that are conducted, and also when the last analysis under this contract is conducted.

III. Analyses

The contractor shall:

a) Briefly describe the number of samples received, the geographic source of the samples, the date they were received, and the laboratory and analytical methods used;

b) Include a table listing the genotypes for all sampled individuals, where each of the 2 microsatellite alleles for each genotyped locus is listed as a letter-based length polymorphism code, in keeping with ISAG standards;

c) Include a table showing, for each set of samples, the frequency of each observed allele, at each of the 12 loci genotyped;

d) Present a table with observed heterozygosity (Ho), expected heterozygosity (He), inbreeding coefficient (Fis), the effective number of alleles (Ae), the mean number of alleles per locus (MNA), the total number of variants (TNV), and the percentage of rare alleles (found at less than 5% frequency; RA).

This table should also include the average, standard deviation, minimum, and maximum of the same measures for average domestic horses (or burros), and feral horses (or burros);

e) Identify whether or not the set of samples contains any unique alleles, not known from other feral or domestic horse samples;

f) Compare estimates of the measures in (b) from the currently analyzed sample set, against available measures from previous sample set(s) from the same area;

g) Present a table with quantitative measures of similarity to common domestic horse (or burro) breed types, including those of New World Iberian ancestry;

h) Present a dendrogram or comparable depiction of quantitative measures of similarity to established domestic breeds;

i) Present a dendrogram or comparable graphical depiction of quantitative measures of similarity to other BLM-managed herds;

j) Not provide management prescriptions or personal commentary about what the contractor thinks BLM should or should not do in terms of wild horse and burro management;

k) Prepare the analysis as a written report in pdf format, deliverable to a designated BLM employee and office, no more than one year from the date after receipt of the sample set or formation of the contract (whichever is later).

IV. Data Ownership

BLM shall be the owner of the intellectual property represented in the raw genotyping data from BLM-managed wild horses and burros. The contractor shall not publish or share genotyping results, database records of genotyped results, or analyses without prior written permission from the BLM Wild Horse and Burro Program Division Chief. The contractor shall not share or publish separate analyses of BLM wild horse and burro genotype results without prior written permission from the BLM Wild Horse and Burro Program Division Chief.

Digitally sent through email to Program Point of contact or COR.

Acceptable data configuration: xcel spreadsheet or comparable

POP: Three Year Period of Performance with two exercisable Option Quantities expected to be exercised in year two and year three.