sf18_75N92022Q0457.pdf

75N92022Q0457

1. REQUEST NO.

5a. ISSUED BY

NAME

a. NAME

c. STREET ADDRESS

d. CITY

10. PLEASE FURNISH QUOTATIONS TO

THE ISSUING OFFICE IN BLOCK 5a ON

OR BEFORE CLOSE OF BUSINESS (Date)

2. DATE ISSUED 3. REQUISITION/PURCHASE REQUEST NO. 4. CERT. FOR NAT. DEF.

UNDER BDSA REG. 2

AND/OR DMS REG.1

RATING

6. DELIVERY BY (Date)

7. DELIVERY

9. DESTINATION

a. NAME OF CONSIGNEE

b. STREET ADDRESS

PAGE OF PAGES

5b. FOR INFORMATION CALL: (No collect calls)

TELEPHONE NUMBER

AREA CODE NUMBER

8. TO:

b. COMPANY

e. STATE f. ZIP CODE

c. CITY

d. STATE e. ZIP CODE

IMPORTANT: This is a request for information, and quotations furnished are not offers. If you are unable to quote, please so indicate on this form and return it to the address in Block 5a. This request does not commit the Government to pay any costs incurred in the preparation of the submission of this quotation or to contract for supplies or services. Supplies are of domestic origin unless otherwise indicated by quoter. Any representations and/or certifications attached to this Request for Quotations must be completed by the quoter.

11. SCHEDULE (Include applicable Federal, State and local taxes)

THIS RFQ

REQUEST FOR QUOTATION

(THIS IS NOT AN ORDER)

IS IS NOT A SMALL BUSINESS SET ASIDEX

08/08/2022 6387245

1 6

National Heart, Lung, and Blood Institutes Bethesda MD 20892-7511

National Institutes of Health 30 Days After Award

BLDG 50, BETHMC

BLDG 50,

Louis Stokes Laboratories, 50 South Drive

Bethesda

MD 20814

Sasha Bradford

08/22/2022 1500 ED

FOB DESTINATION

OTHER

(See Schedule)X

ITEM NO.

(a)

SUPPLIES/SERVICES

(b)

QUANTITY

(c)

UNIT

(d)

UNIT PRICE

(e)

AMOUNT

(f)

12. DISCOUNT FOR PROMPT PAYMENT

a. 10 CALENDAR DAYS (%) b. 20 CALENDAR DAYS (%) c. 30 CALENDAR DAYS (%) d. CALENDAR DAYS

NUMBER PERCENTAGE

NOTE: Additional provisions and representations

13. NAME AND ADDRESS OF QUOTER

b. STREET ADDRESS

c. COUNTY

d. CITY e. STATE f. ZIP CODE

14. SIGNATURE OF PERSON AUTHORIZED TO

SIGN QUOTATION

16. SIGNER

a. NAME (Type or print)

c. TITLE (Type or print)

a. NAME OF QUOTER

AREA CODE

NUMBER

15. DATE OF QUOTATION

b. TELEPHONE are are not attached

AUTHORIZED FOR LOCAL REPRODUCTION

Previous edition not usable

STANDARD FORM 18 (REV. 6-95)

Prescribed by GSA - FAR (48 CFR) 53.215-1(a)

COMBINED SYNOPSIS / SOLICITATION

WORKFORM

(1) Action Code: Combined Synopsis/Solicitation

(2) Date: 08/08/2022

(3) Year: 2022

(4) Contracting Office Zip Code: 20892

(5) Classification Code: 6640

(6) Contracting Office Address:

6705 Rockledge Drive

RKL I

Bethesda, MD 20892

(7) Subject/Title: 10X Genomics Chromium X

(8) Proposed Solicitation Number: 75N92022Q0457

(9) Closing Response Date: 08/22/2022

(10) Contact Point(s): Elizabeth Francois, Contracting Officer

(11) Contract Award and Solicitation Number: TBD

(12) Contract Award Dollar Amount: TBD

(13) Contract Line Item Number(s): TBD

(14) Contractor Award Date: TBD

(15) Contractor Name: TBD

(16) Description: TBD

(i) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6 as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a separate written solicitation will not be issued.

(ii) The solicitation number is 75N92022Q0457 and the solicitation is issued as a request for quotation (RFQ).

(iii) The solicitation document, the incorporated provisions and clauses are those in effect through Federal Acquisition Circular 2022-06 effective 05/26/2022.

(iv) This requirement has no set-aside restrictions. The associated NAICS code is

334516 Analytical Laboratory Instrument and Manufacturing, small business size standard is 1000 employees. The acquisition is being conducted in accordance with the procedures of FAR Part 12 and FAR Part 13.

(v) It is anticipated that there will be two (2) or more contract line items listing all components. The specifications and quantities of required items are listed below.

(vi) Statement of Need

Background

The National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) Genomic Technology Section (GTS) is charged with providing genome-scale, high throughput sequencing services for researchers within the institute.

The Core Facility, like most other Institutes at NIH and sequencing centers around the world, adopts new emerging technologies to maintain the leading edge on genomics and epigenetic research areas.

GTS acquired the 10X Genomics Chromium Controller Single Cell Platform in 2020 (75N92020P00151) to support a study of the biology and cellular processes influenced by SARS-CoV-2, and to access technology that enables the discovery of gene expression dynamics and molecular profiling of individual cell types. The equipment is currently used as a molecular barcoding and analysis suite that delivers cell-by-cell 3’ counting of mRNA transcripts for many tens of thousands of cells per run. The platform supports single-cell spatial transcriptomics, cancer-cell transcriptomics and cell-type identification and discovery.

Due to the program’s increasing demand for single-cell experiments, GTS is requesting to purchase the 10X Genomics Chromium X. This equipment as the Chromium Controller, uses technology that partitions reactions into nanoliter-scale droplets containing uniquely barcoded beads called Gel Bead-In Emulsions (GEMS) at the single cell level. The main difference between these instruments is that the Chromium X has higher throughput and allows to capture higher number of cells per experiment. Because both instruments work with short read sequencers and are compatible with GTS existing Illumina sequencers because they use the same primers for sequencing and to generate the libraries. Some of the Chromium X high- throughput assays that NIAMS investigators are requesting to advance the research include Single Cell Immune Profiling, Single Cell Gene Expression, Single Cell Multiome ATAC + Gene Expression, Single Cell ATAC and Targeted Gene Expression. The Chromium X will be an essential tool to support NIAMS broad range of basic and clinical research applications.

Specifications

The following product features/characteristics are required for this requirement:

I. Generate single-cell transcript counts from up to 80,000 cells per run.

II. Produce >90,000 individual nanoliter-scale partitions leveraging ~3.6 million unique barcodes (~10k unique barcodes for LT).

III. Capture cells at high efficiency up to 65% (up to 35% for LT) Low doublet rate of 0.9% per 1,000 cells (~8% for LT, ~0.5% for HT).

IV. Achieve deep profiling of complex cell populations with high-throughput digital gene expression of individual cells.

V. Access the whole transcriptome with an unbiased approach appropriates for discovery work, or focus on a subset of relevant target genes by performing target enrichment of the same library sample prior to sequencing.

VI. Measure additional phenotypes with Feature Barcode technology (including CellPlex).

VII. Provide turn-key analysis pipelines and visualization software for novice and expert users alike.

VIII. Include a microfluidics chip that is compatible with a wide range of eukaryotic cell sizes and types (e.g. adherent cell cultures, suspension cell cultures, tissues, blood, etc.).

IX. Rapid time to lysis provides an accurate view of the transcriptome with minimal impact on expression.

Instrument parameters and specifications:

• Weight: 41.4 lb (18.8 kg)

• Dimensions: W x D x H 11.25” [28.6 cm] x 19” [48.3 cm] x 10.75”

[27.3 cm]

• Electrical Requirements: 100–240 VAC, 50/60 Hz, 250 W

• Operating Temperature: 64–82°F [18–28°C]

• Humidity: 30–80% R.H. non-condensing

• Altitude: Up to 7500 ft [2286 m] above sea level

• Power cable length: 6–7 ft [2 m] Standard - country specific adaptors included

Other Requirements

Instrument training of four (4) users should occur within seven (7) days of equipment installation.

(vii) The provision at FAR clause 52.211-6 - Brand Name or Equal, applies to this acquisition.

(viii) Delivery and acceptance and FOB Point:

NIAMS/ Genomic Technology Section (GTS) Building 50, Room 1342 9000 Rockville Pike

(ix) Provision at FAR clause 52.212-1, Instructions to Offerors – Commercial Items, applies to this acquisition.

• The Government requests discounts

• The required items must meet the brand name or equal specifications

• Quotes shall include 12 months manufacturer warranty, delivery, and installation/assembly

• Delivery of all products must be received within 30 days after award.

• Quotes must include itemized prices

• Shipping costs must be included

(x) The provision at FAR clause 52.212-2, Evaluation – Commercial Items, is applicable to this acquisition.

• The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers:

o Offeror has provided products/services as described in section (vi) Statement of Need.

o Offeror must meet the required specifications or better.

o Offeror can deliver and install all products within thirty (30) days from award.

o Offeror quote includes 12 months manufacturer warranty, delivery and installation/assembly o Quotes include itemized prices.

(xi) Offerors to include a completed copy of the provision at FAR clause 52.212-3, Offeror Representations and Certifications – Commercial Items, with offer. An offeror shall complete only paragraphs (b) of this provision if the offeror has completed the annual representations and certificates electronically via http://www.acquisition.gov. If an offeror has not completed the annual representations and certifications electronically at the System for Award Management (SAM) website, the offeror shall complete only paragraphs (c) through (p) of this provision.

(xii) The FAR clause at 52.212-4, Contract Terms and Conditions – Commercial

Items, applies to this acquisition.

(xiii) The FAR clause at 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders – Commercial Items, applies to this acquisition.

• 52.204-25, Prohibition on Contracting for Certain Telecommunications and Video Surveillance Services or Equipment. (AUG 2019) (Section 889(a)(1)(A) of Pub. L. 115-232).

• 52.222-3, Convict Labor (June 2003) (E.O. 11755)

• 52.222-21, Prohibition of Segregated Facilities (APR 2015)

• 52.222-26, Equal Opportunity (APR 2015) (E.O. 11246)

• 52.222-36, Equal Opportunity for Workers with Disabilities (JUL

2014) (29 U.S.C. 793)

• 52.222-50, Combating Trafficking in Persons (MAR 2015). (22

U.S.C. chapter 78 and E.O. 13627)

• 52.223-18, Encouraging Contractor Policies to Ban Text

Messaging While Driving (AUG 2011) (E.O. 13513)

• 52.225-13, Restrictions on Certain Foreign Purchases (JUN 2008)

• 52.232-33, Payment by Electronic Funds Transfer—System for

Award Management (JUL 2013) (31 U.S.C. 3332)

(xiv) Additional contract requirement(s): N/A

(xv) The Defense Priorities and Allocations System (DPAS) and assigned rating are not applicable.

(xvi) Offers shall be submitted via the FedConnect web portal (www.fedconnect.net).

The due date for receipt of offers is 15:00 PM Eastern Time on 08/22/2022.

http://www.fedconnect.net/

(xvi) Point of Contact:

Elizabeth Francois, Contracting Officer www.fedconnect.net solicitation 75N92022Q0457

(17) Place of Contract Performance:

NIAMS/ Genomic Technology Section (GTS) Building 50, Room 1342 9000 Rockville Pike

(18) Set-aside Status: N/A http://www.fedconnect.net/