SB1341-16-RQ-0378_SOW.pdf

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SYSTEM SUMMARY DESCRIPTION. The National Institute of Standards and Technology (NIST) has a requirement for the purchase of an Ultra High Pressure Liquid Chromatograph (Thermo Scientific Vanquish comprising (Model# VH-S01-A, Model# VH-P10-A, and Accucore Vanquish C18+ 1.5 micrometer column (Catalog # 27101-102130) system that can be directly integrated into NIST’s existing Thermo Fisher hydrogen-deuterium exchange mass spectrometry (HDX-MS) system. This HDX-MS system comprises a LEAP HDX PAL robot, Thermo Fisher 3000 chromatograph that uses a cold-box four-valve system, and a Thermo Fisher Orbitrap Elite that is operated through resident software comprising LEAP HDX-Director, Thermo Scientific Chromeleon, Thermo Scientific Xcalibur, and Thermo Scientific DCMS link software packages.

The delivered system shall include an optical spectroscopic detection system. The vendor shall deliver, install, and provide training for operating the delivered system. The delivered equipment shall also include the Thermo Fisher branded software licenses and keys (Model# 1359060, Model# 088617, and Model# 7000.0067, and Model# 7050.0104A)) that allow the supplied six pumps to operate as the chromatograph in the Thermo Fisher HDX-MS system. In addition, the vendor shall provide a Thermo Fisher branded software license and key (Model# 7000.0067 and Model# 7050.0104A) that will allow the existing Thermo Fisher 3000 chromatograph to operate as a stand-alone chromatograph with the supplied optical detector (Model# 5074.0010 and Model# 6074.0290). The vendor will provide a 12-month warranty for parts, labor, and travel associated with repair of the delivered components that includes one preventative maintenance visit. For the components covered under the 12-month warranty, the vendor shall offer two option years that provide maintenance for repair of the delivered components including one preventative maintenance visit per year.

SPECIFIC SYSTEM SPECIFICATIONS. The Thermo Fisher branded Extremely High Pressure Liquid Chromatograph System must meet or exceed the following salient characteristics:

1.0 Liquid Chromatograph.

1.1 System configuration shall have the Thermo Electron North America LLC parts:

1.1.1 One Model# VH-P10-A , Binary Pump H

1.1.2 One Model# VH-S01-A, SYSTEM BASE VANQUISH including one

Accucore Vanquish C18+ 1.5 micrometer column (Thermo Scientific Catalog # 27101-102130).

1.1.3 One Model# 5035.9230 SRD-3600 SOLVENT RACK

1.1.4 Two Model# 5040.0066, DPG-3600RS PUMP Systems

1.1.5 One Model# 5040.0011, ISO-3100SD PUMP

1.1.6 One Model# 5074.0010, VWD-3400RS Detector, without cell

1.1.7 One Model# 6074.0290, FLOW CELL, UZ-VIEW,180NL, VF-D4, VWD

1.1.8 Model# 6000.1004, 6-PIN MINI-DIN SIGNAL CABLE(5M)

1.1.9 Two Model# 7050.0104A, CM7 LICENSE KEY: NEW

1.1.10 Two Model# 7000.0067, Chromeleon 7 Bundle WSO Workstation Open

1.1.11 One Model# 1359060, Software OT Elite Instr. 2.7 SP2_DVD

1.1.12 One Model# 088617, PROD, SW, SII-XCALIBUR,DVD

1.1.13 Four Model# 6041.5711 CAPILLRY, NANOVIPER,1/32IN, IDXL

75UMX1100

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1.1.14 One Model# 6041.5785 NANOVIPER CAPILLARY IDXL

75UMX850MM

1.1.15 One Model# 6040.5110 STATIC MIXER 150UL (FOR 200UL), 150MPA

1.1.16 One Model# 6040.5000, Mixer for 35UL, SST,RS/SD Pumps

1.1.17 Model# LCCONSUMABLESTMO14, LC CONSUMABLES VOUCHER

1.1.18 Six Model# 6000.1001, POWER CORD USA-VERSION

1.2 The system will include solvent racks (Model# 5035.9230 and Model# VH-S01-A) that hold reservoirs for all chromatographic fluids.

1.3 The system shall contain degassing facilities for all solvents flowing into each pump.

1.4 The system shall include provisions (Model# LCCONSUMABLESTMO14) for connectors and capillaries between the pumps, valves, mixer, and the optical and mass detectors.

1.5 The system shall include tubing from the six pumps to the valve system connections (ref. spec. 1.1.13, 1.1.14, & 1.1.17). These connections will be terminated by zero dead-volume connectors.

1.6 The system shall be delivered with one Accucore Vanquish C18+ (Thermo Scientific Catalog # 27101-102130) analytical column containing 1.5 micrometer pores (or beads) with inner dimensions of 2.1 mm bore diameter x100 mm long.

1.7 The supplied system shall contain a 150 microliter static mixer (Model# 6040.5110) that mixes the solutions driven by two pumps.

1.7.1 To attain complete mixing, the mixer shall employ two-step process of radial mixing followed by longitudinal mixing.

1.7.2 The mixer shall operate without leaks or loss of physical integrity at pressures up to 1034 bar (15,000 psi).

1.8 The supplied system will contain a 35 microliter static mixer (Model# 6040.5000) that mixes the solutions driven by two pumps.

1.8.1 To attain complete mixing, the mixer shall employ two-step process of radial mixing followed by longitudinal mixing.

1.8.2 The mixer shall operate without leaks or loss of physical integrity at pressures up to 1034 bar (15,000 psi).

1.9 DPG-3600RS pumps (Quantity=2, Model# 5040.0066)

1.9.1 The system shall contain four pumps that tolerate mixtures containing dimethylformamide, ethanol, ethylene glycol, formamide, acetonitrile, methanol, and water, salts of sulfate, hydrogen phosphate, ammonium, and sodium.

1.9.2 Each pump shall provide compressibility compensation.

1.9.3 Each pump shall deliver flows at user-selected flow rates between 0.001 mL/min to 5.0 mL/min at pressures up to 1034 bar (15,000 psi).

1.9.4 Flow precision of each pump shall be better than or equal to 0.05% relative standard deviation (RSD) or 0.01 min standard deviation (SD), whichever is greater.

1.9.5 Each pump shall produce pulsation of the solvent pressure that is less than the greater of 2 bar or 1% of total pressure.

1.9.6 Each pump shall have a flow accuracy of +/-0.1% or better.

1.9.7 When mixing proportional fractions of solvent flow, the standard deviation of the proportioning precision for solvents shall be better than or

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1.9.8 The chassis electronics for the pumps shall have an input/output interface comprising two digital inputs (nominal TTL or similar) and two relay outputs.

1.9.9 The pumping components are biocompatible between pH 2 and pH 12 and for chloride concentrations up to 1 mol/L.

1.10 High Pressure Binary Pump H (Model# VH-P10-A)

1.10.1 The system shall contain a binary pump system that can prepare gradients mixtures containing water, methanol, ethanol, ethylene glycol, formamide, dimethylformamide, acetonitrile with user-selected flows of (0.001 to 5.000) milliliter per minute (mL/min) at pressures up to 1500 bar (21,755 psi).

1.10.1.1 Flow rate range shall be between 0.001 mL/min and 5

mL/min, which the user can adjust in 1 microliter per minute (μL/min) increments.

1.10.1.2 Pump flow accuracy shall be +/-0.1%.

1.10.1.3 Flow precision shall be better than 0.05% relative standard deviation (RSD) or less than 0.01 min standard deviation (SD), whichever is greater.

1.10.1.4 The binary pump system shall provide compressibility compensation.

1.10.1.5 The binary pump shall produce pulsation of the solvent pressure that is better than the greater of 2 bar or 0.4% of total pressure.

1.10.1.6 Proportioning accuracy of the binary solvents shall be at least +/-0.2% of full-scale for flows ranging from 0.2 mL/min to

4.0 mL/min and proportions of 1% to 99%.

1.10.2 Each pump chassis electronics shall have an input/output interface comprising of at least two digital inputs (nominal TTL or similar) and two relay outputs.

1.10.3 The pump system shall include a solvent mixer, solvent degassers, an automated purge unit, and all installation accessories necessary for turnkey operation.

1.10.4 The controlling firmware/software shall provide leak detection, safe leak handling, and monitor for excess pressure.

The pumping components are biocompatible between pH 2 and pH 12 and for chloride concentrations up to 1 mol/L.

1.11 ISO-3100SD Isocratic Pump (Model# 5040.0011)

1.11.1 The pump shall tolerate without damage and operate with solutions containing predominantly acetonitrile, ethanol, methanol, and water with 2% to 40% concentrations of one component of the following: 1,2-butylene carbonate, ethylene carbonate, 4-hydroxymethyl-1,3-dioxolan-2-ene, 3-nitrobenzonitrile, m-nitrobenzyl alcohol, o-nitroanisole, propylene carbonate, sulfolane, 2-thiophenone, 4-vinyl-1,3-dioxolan-2-one.

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1.11.2 The pump shall deliver flows at user-selected flow rates between 0.001 mL/min to 10.000 mL/min at pressures up to 620 bar (8992 psi).

1.11.3 Pump flow accuracy shall be +/-0.1%.

1.11.4 Flow precision shall be better than 0.05% RSD or less than 0.01 min SD, whichever is greater.

1.11.5 The pump shall produce pulsation of the solvent pressure that is better than the greater of 2 bar or 1% of total pressure.

1.11.6 Each pump chassis electronics shall have an input/output interface comprising of at least two digital inputs (nominal TTL or similar) and two relay outputs.

2.0 Eluent Spectroscopic Detector (UltiMate 3000 VWD-3400RS) (Model# 5074.0010)

2.1 The system shall contain a multi-wavelength detector module for simultaneous absorbance detection at four user-selected wavelengths spanning 190–800 nm.

2.2 The monochromator shall contain optical filters to reduce noise and ghosts from higher order light.

2.3 The electronics shall be able to acquire data with user selected rates up to 200 Hz.

2.4 Stability specifications of drift and noise shall be attained within 60 minutes from powering up.

2.4.1 As measured at 254 nm in a dry analytical flow cell, drift shall be better than or equal to +/-0.0001 airy unit (AU) per hour.

2.4.2 As measured at 520 nm in a dry analytical flow cell, drift shall be better than or equal to +/-0.0001 AU per hour.

2.4.3 Noise shall be better than +/-10 micro-AU at 254 nm and 280 nm for a time constant of 2 s and data collection rate of 2 Hz.

2.4.4 Noise shall be better than +/-10 micro-AU at 450 nm and 520 nm for a time constant of 2 s and data collection rate of 2 Hz.

2.5 Linearity shall be better than 5% RSD at 272 nm.

2.6 Linearity shall be better than 5% RSD at 520 nm.

2.7 Optical Cell (Model# 6074.0290) properties

2.7.1 The optical cell shall have a working volume of 180 nanoliters.

2.7.2 The optical cell components are biocompatible between pH 2 and pH 12 and for chloride concentrations up to 1 mol/L.

2.7.3 The windows of the optical cell shall pass light sufficient to allow the system to operate between 190–800 nm.

3.0 Controls, Displays, Software, and Computer control

3.1 The front panel of each pump unit chassis shall indicate system information (e.g., device type, serial number, and bootloader and firmware versions), progress/results of diagnostic tests, pump status (Power, Connected, Status, Ready), and operational parameters (e.g., flow rate, pressure, components of the solvent in percent of the total flow).

3.2 Software licenses.

3.2.1 The delivered equipment shall also include software licenses and keys

(Model# 1359060, Model# 088617, Model# 7000.0067, and Model# 7050.0104A) that allow components supplied under this acquisition

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(specifically indicated in Spec. 1.1.1, 1.1.2, 1.1.3, 1.1.4, 1.1.5, 1.1.6, 1.1.16) to operate as the chromatograph of the Thermo Fisher HDX-MS system (when combined with HDX valve system).

3.2.2 Separate from Spec 3.2.1, the vendor shall provide a software license and key (Model# 7000.0067 and Model# 7050.0104A) that will allow the existing Thermo Fisher 3000 chromatograph (Serial # 8067655) to operate as a stand-alone chromatograph with the supplied optical detector (Model#

5074.0010 and Model# 6074.0290).

3.2.3 The vendor shall provide one copy of Model# 088617, PROD,SW,SII- XCALIBUR on DVD media.

3.2.4 The vendor shall provide one copy of Model# 1359060, Software OT Elite Instr. 2.7 SP2 on DVD media.

3.3 The front panel of each pump unit chassis shall have a button that can put the unit into “Standby” mode.

3.4 All delivered pumps and detectors shall be controllable through the Chromeleon 7.2 and link software that communicates with the Xcalibur 2.2 SP1 system.

3.5 The supplied software shall be compatible with a user-supplied, dual processor computer operating on Microsoft Windows 7 SP1 64-bit or Microsoft Windows 8.1 64-bit operating systems.

3.6 All active pumps and components of the chromatographic system shall be software-controlled through a standard USB connector with the computer.

3.6.1 All pumps shall provide full support of Thermo Scientific™ Dionex™ AutoQ instrument qualification that logs and reports qualification status, system wellness, and the occurrence of leaks and operational malfunctions.

3.6.2 All pump functions shall be controllable via software through the USB connection to the computer.

3.6.3 The vendor shall provide a five metter long, 6-pin mini-DIN signal cable (Model# 6000.1004).

3.6.4 Each pump shall be able to sense fault conditions. When a fault condition is sensed, the operating software shall place the pumps into idle modes that may include 0.005 to 0.05 microliters/min solvent flow.

4.0 Performance and Acceptance Tests

4.1 During the installation the technician shall demonstrate system performance with respect to pump operation, software control of pumps and error messages.

4.2 The technician shall demonstrate pumping of degassed solvents from each pump.

5.0 Physical, Electrical and Environmental Properties

5.1 The extremely high pressure liquid chromatograph system shall operate with power from the 115V, 15 ampere mains.

5.2 Power cords to all electrical components shall be supplied by the vendor (quantity = 6 Model# 6000.1001).

5.3 All components shall operate within specifications within the environmental parameters of 10 °C to 35 °C and up to 80% relative humidity (non-condensing).

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6.0 Warranties and Licenses.

6.1 The vendor will provide a 12-month warranty for parts, labor, and travel associated with repair of the delivered components that includes one preventative maintenance visit. The warranty shall not commence until installation, training and final acceptance at IBBR in Rockville, MD.

6.1.1 The warranty must include unlimited telephone/e-mail support for questions regarding operation.

6.1.2 All personnel conducting maintenance and warranty service shall be trained by the manufacturer and certified to be qualified to service the delivered system components.

6.1.3 All costs including parts, labor, travel, and other expenses necessary to repair the system will be borne solely by the contractor at no additional cost to the U.S. Government.

6.2 The contractor shall provide two option years of maintenance services to begin after the expiration of the equipment warranty. Maintenance services shall cover the same support, maintenance, and repair terms as the warranty requirements.

6.3 The vendor must deliver manuals, in printed or PDF format, that specify chromatographic system modes of operation, operating parameters, set-up procedures, preventive maintenance procedures, troubleshooting procedures.

6.4 NIST will retain unlimited, nonexclusive licenses to use the supplied software.

6.4.1 NIST/IBBR shall have the unlimited right to install all software associated with the operation and analysis of the chromatography system on institutional computers free of charge.

6.4.2 All future software version updates associated with this instrument shall be made available to NIST/IBBR free.

6.5 The government shall retain all rights to all drawings and files provided by the vendor.

7.0 Delivery, Installation, and Acceptance Tests.

7.1 Delivery shall be completed not later than five (5) weeks after award.

7.2 Delivery shall be to the FOB Destination to the address below

7.2.1 FOB Destination means: The contractor shall pack and mark the shipment in conformance with carrier requirements, deliver the shipment in good order and condition to the point of delivery specified in the purchase order, be responsible for any loss of and/or damage to the goods occurring before receipt and acceptance of the shipment by the consignee at the delivery point specified in the purchase order; and pay all charges to the specified point of delivery. The contractor shall deliver the system to Institute for Bioscience and Biotechnology Research (IBBR), Deliver to:

Institute for Bioscience and Biotechnology Research 9600 Gudelsky Drive, Loading Dock 2 Rockville, MD 20850

7.2.2 The installation shall occur no later than two weeks after delivery. The installation schedule shall be coordinated with the NIST Technical Point of Contact (TPOC). Once the system has been delivered, the date of

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NIST COR.

8.0 Training.

8.1 The contractor shall conduct one (1) training session for up to four (4) technical personnel at NIST at the on-site at Institute for Bioscience and Biotechnology Research (IBBR) in Rockville, MD. The training shall provide a thorough demonstration of all system/solution functions, maintenance, troubleshooting and hardware/software operation, leak detection, and minor system modifications. The training may be completed at NIST immediately after installation/set-up and demonstration of performance specifications, but shall be completed no later than 15 days after installation (ref. Section 5.0).

8.2 Training must be scheduled with the NIST TPOC.