SB1341-16-RQ-0309_StatementOfWork.pdf

STATEMENT OF WORK

TITLE: Acquisition of Liquid Chromatography and High Resolution Mass Spectrometry Instruments

LAB REQUESTING SERVICE: Materials Measurement Laboratory, Materials Measurement Science Division

I. BACKGROUND INFORMATION

The National Institute of Standards and Technology’s Chemical Sciences Division serves as the Nation's reference laboratory for chemical measurements and standards to enhance U.S. industry's productivity and competitiveness, assure equity in trade, and provide quality assurance for chemical measurements used for assessing and improving public health, safety, and the environment. The Chemical Sciences Division is responsible for development and characterization of Standard Reference Materials (SRMs) to enhance chemical measurement comparability within the U.S. and to provide a basis for measurement comparability with other nations. SRMs are provided to support clinical, food, environmental, fuel, metal alloy, cement, and advanced materials analyses. Research activities are geared toward improvement of measurement capabilities to address U.S. industry and government agencies' requirements for new measurement services and reference materials. Current research focus areas include the development of measurements and standards for determination of protein-based health markers, chemical characterization of complex matrices (e.g. lipidomics and metabolomics), and for environmental contaminant analysis.

The Chemical Services Division maintains core expertise in:

• Analytical mass spectrometry,

• Analytical separation science,

• Atomic and x-ray fluorescence spectrometry,

• Gas metrology,

• Nuclear analytical methods,

• Nuclear magnetic resonance spectrometry, and

• Classical and electroanalytical chemistry.

Because of NIST’s role as the National Metrology Institute (NMI) in the U.S., the Division’s measurement capabilities must be benchmarked against the capabilities of NMIs worldwide as required by a Mutual Recognition Arrangement (MRA) established in 1999. The MRA provides a framework for obtaining reliable quantitative information on the comparability of measurement services provided by all NMIs, thereby providing governments and other parties with a technical foundation for wider agreements related to international trade, commerce, and regulatory affairs.

The Division actively participates in the International Committee of Weights and Measures (CIPM) Consultative Committee for Amount of Substance – Metrology in Chemistry (CCQM) comparisons, which address chemical measurement-related issues important for international trade, environmental, health, and safety-related decision making.

II. SCOPE OF WORK

NIST intends to acquire two ultra-performance high-pressure liquid chromatography (UHPLC) systems and a high resolution mass spectrometer used for the separation and identification of proteins by intact mass analysis, peptide mass fingerprinting, identification and structural elucidation of posttranslational modification (e.g. phosphorylation and glycosylation), metabolite and lipid profiling, and the quantification of peptides, proteins, lipids, metabolites, and contaminants. The instrument must be able to reproducibly generate high resolution and high quality MS and MS/MS in a variety of fragmentation modes in order to accurately identify unknown analytes in complex matrices.

This is a brand name or equal requirement. Brand name or equal description is intended to be descriptive, but not restrictive and is to indicate the quality and characteristic of products that will be considered satisfactory to meet the agency’s requirement. Additionally, Refurbished or Remanufactured brand name or equivalent equipment is acceptable.

The required equipment will be used for high resolution chromatographic separations and mass spectrometric measurements. The required equipment consists of benchtop instruments with integrated instrument control and data analysis software.

III. SPECIFICATIONS

The Contractor shall provide the following new or manufacturer refurbished Thermo-Fisher branded equipment or equivalent, and software for complete instrument system control and data analysis capabilities meeting the salient characteristics as defined below:

Part Number Instrument (and Components)

Instrument Description

Specifications

5400.0105 LSMS Bundle, Vanquish Binary w/o Detector

Vanquish System Base

Vanquish UHPLC Binary Pump w/ solvent degasser

Pressure 50 - 1500 bar

Flow Rate 1 L/min – 3000 L/min

Flow Rate Accuracy 0.10% pH range 2-12

Vanquish UHPLC Autosampler HT

Controlled temperature environment 4 to 40 C

Operating pressure up to 1500 bar

Injection volume 1-100 L

Injection accuracy 0.5% or better

Sample carryover < 0.05%

Vanquish Column Compartment

Temperature control 5 ˚C up to 50 ˚C

Operating pressure 1500 bar

Column Dimension Holding Capability 300 mm length and 4.6 mm i.d.

Vanquish MS Connection Kit

Vanquish Consumable Kit

5200.0355 Ultimate 3000 Binary RS Nano Pump with Sample Loading Pump

Ultimate 3000 Binary Pump

Pressure 700 bar (sample loading 500 bar)

Flow Rate

20 nL/min - 500 nL/min without splitting from a higher flow rate

Flow Rate Proportioning accuracy 1%

Flow Rate Proportioning precision 0.5

Ultimate 3000 Autosampler

Controlled temperature environment 4 to 45 C

Operating pressure up to 600 bar

Injection volume 50 nL to 10 µL

Injection accuracy 0.5% or better

Sample carryover < 0.05%

Injection Modes full or partial loop

Ultimate 3000 Column Compartment w/ column switching valve

Temperature control ambient + 5 ˚C to 40 ˚C

Operating pressure 1000 bar

Column switching valve pressure 1000 bar

Ultimate 3000 Solvent Degasser

Ultimate 3000 MS Connection Kit

Ultimate 3000 Consumable Kit

ES269 UHPLC Junction Kit

EXRFSN02-

10002

Orbitrap Fusion Lumos ETD Mass Spectrometer

Orbitrap Fusion Lumos Tribrid Instrument

Architecture

Quadrupole mass filter, Linear Ion Trap, Orbitrap mass spectrometer

Tribrid Operation Sequentially or in Parallel

Footprint must not exceed the dimensions of 30 in x 55 in x 32 in (height x width x depth)

Resolution 500,000 (FWHM) @ 200 m/z

180,000 (FWHM) @ 1500 m/z

Dynamic range (full MS scan) 5,000

Sensitivity (signal to noise) 150:1 of 100 fg reserpine

Mass Accuracy 3 ppm (external)

MS/MS Fragmentation collision-induced fragmentation (CID), electron transfer (ETD), and higher energy collisional dissociation (HCD)

Fusion Lumos Consumable Kit

Xcalibur Instrument Control Software (or current available version) Software

Instrument control all of the MS and UHPLC components and data processing of MS and MS/MS generated data.

FETD2-10002 ETD Lumos Kit (Factory Installation)

(requires FETD2-10001 to be included)

OPTON-20045 Fusion Lumos Installation Kit

ES072 Fusion Lumos Nanospray Flex Ion Source (NG)

Nanospray Flex Ion Source

Operating Voltage up to ± 5000 V (100 µamps)

Liquid Flow Rate Operation Range 50 nL/min – 1000 nL/min

Source Adjustment adjustable in the x, y and z dimensions relative to the sample cone orifice

OPTON-30156 Ion-Max HESI Ion Source (NG)

Fusion Lumos HESI Source

Operation Range 1 µL/min – 1500 µL/min

Temperature up to 500 ºC

OPTON-30159 Fusion Lumos APCI Ion Source (NG)

Fusion Lumos APCI Source

Operation Range 1 µL/min – 1500 µL/min

Temperature up to 500 ºC

OPTON-30376

Proteome Discoverer 2.0 w/ Quantification (or current available version)

Software

Identification of proteins and peptides via database searching of instrument generated MS and MS/MS spectra and quantification by MS

OPTON-30389 Compound Discoverer 1.0 (or current available version)

Software

Identification of metabolites via database searching of instrument generated MS and MS/MS spectra

OPTON-30285 Tracefinder 3.2 (or current available version)

Software

Perform targeted screening and quantification and reporting capability

702-027700 3-day LCMS Training Course (customer site)

The instrument software must be compatible with Windows 7 operating systems. The instrument software must be able to control and seamlessly integrate all of the MS and LC functionality with a single software program/package.

Additionally, the contractor shall provide copies of the operations and maintenance manuals for all equipment covering basic operation, maintenance procedures and troubleshooting.

If the system is refurbished, the contractor shall provide a letter or certificate verifying that the system has been refurbished by the manufacturer.

Warranty

The Contractor shall provide, at a minimum, a one year warranty for the system’s non-consumable parts. The warranty provided shall cover all of the following:

- Parts, labor, and travel;

- Software and firmware updates;

- Unlimited, free email and phone support;

- In the event that NIST is unable to resolve a problem, the Contractor shall provide remote support as needed to expedite a solution for NIST;

- Telephone support for the covered equipment during regular business hours; Telephone support must include response to questions about operating practices, application use, and service issues that can be diagnosed and resolved over the telephone.

The warranty shall commence upon successful completion of delivery and demonstration of all salient characteristics.

Installation and Training

The contractor shall install the equipment. The contractor shall schedule and facilitate installation/set-up and one (1) advanced user training session of a minimum of three days (3) for up to four (4) users at NIST. The training shall provide a thorough demonstration of all equipment functions, maintenance, data administration, data acquisition, and basic troubleshooting. The training may be completed at NIST immediately after installation/set-up and on-site measurements demonstrating that no damage or misalignment issues arose during transportation and installation (as described below), but shall be completed no later than 30 days after delivery.

Prior to completion of the installation, the Contractor shall test the equipment, review the equipment’s performance, and take whatever steps are necessary to ensure the equipment is functioning in accordance with the manufacturers’ specifications.

System Updates

All software updates issued by the manufacturer during the warranty period, developed for the purposes of instrument optimization, should be made available no later than 60 days after any such update is released. Updates should be installed by either the contractor and/or NIST personnel.

IV. PERIOD OF PERFORMANCE

Delivery, installation, training and acceptance testing shall occur not later than 120 days from the date of the award.

V. PLACE OF PERFORMANCE

Delivery, installation, training and warranty services on the instruments shall occur at NIST, Charleston, SC. Normal duty hours are Monday through Friday, 8:30 a.m. to 5:00 p.m. with the exception of Federal holidays.

DELIVERABLES The contractor shall ship the instrumentation to the specified address below in accordance with the timeframe specified in the deliverable table below. FOB Destination shipping terms shall apply.

NIST,

ATTN: John Bowden 331 Fort Johnson Road, Hollings Marine Laboratory, Charleston SC, 29412.

Description Quantity Due Date Analytical instrumentation and operations and maintenance manuals and certification of manufacturer refurbishment (if applicable)

One (1) 60 days after award of this requirement.

Installation of instrumentation One (1) 30 days after delivery of the instrumentation

Advanced operations and maintenance training for the analytical instrument

One (1) 30 days after successful installation of this requirement

VI. ACCEPTANCE CRITERIA

After installation of the instrumentation, the contractor shall demonstrate all minimum required specifications. The demonstration of specifications may occur during training. Final acceptance by the Government will be given after successful delivery, installation, training and demonstration of instrumentation specifications.