SATPC0041909 Tab 04 4 SOW .pdf

Moderate Altitude testing STATEMENT OF WORK

The vendor will provide physiological and cognitive test data collection for 12 participants to characterize health and performance outcomes of low and moderate altitude exposure. The data collection will include data from one (1) week at low altitude (LA) for baseline measurements followed by four (4) consecutive weeks at a moderate altitude (MA, ~2300 meters). The vendor will screen and select the study participants and share the relevant demographics and health data with

NASA.

Data collection, data delivery and sample collection will be completed by December 1, 2026. See data collection descriptions and schedule below.

Test Descriptions Cognitive Performance Cognitive performance will be quantified using the Automated Neuropsychological Assessment Metrics (ANAM), which is a suite of computer-based tests designed to measure cognitive function at a single time-point or longitudinally to detect changes in cognitive function. A total of 6 sessions will be completed at low altitude before ascending to moderate altitude. Subjects first will participate in a 60-minute familiarization session at the vendor’s location. For subsequent sessions, subjects will perform ANAM once per day (30 minutes) at approximately same time of day. After the familiarization session, subjects will self-administer two tests at home before returning to the facility after the familiarization, and 3 baseline measures will be collected at the facility at low altitude. See schedule below for detailed schedule of low and high-altitude test sessions.

Assessment of Acute Mountain Sickness and Other Symptoms To quantify hypoxia-related symptomatology, participants will complete the Lake Louise Acute Mountain Sickness (AMS) scoring questionnaire at low altitude and at moderate altitude. The AMS score reflects the sum of 5 symptom items rated on a 0-3 scale. Consistent with standard criteria, AMS is diagnosed when a participant reports a headache plus at least 1 additional symptom – gastrointestinal symptoms, fatigue and/or weakness, dizziness and/or lightheadedness – with a total score of 3 points or greater. See details of testing schedule below.

In addition to these structured measures, test operators and the attending medical officer(s) will document the occurrence, time, and date symptoms reported during the study, whether elicited during scheduled assessments or spontaneously communicated by participants. This combined approach ensures comprehensive capture of both expected and unexpected manifestations of acute hypobaric hypoxia.

Sleep Quality and Duration Participants will wear an actigraphy monitor wristband or lanyard continuously during the low altitude baseline measurement days and during the moderate altitude exposure to identify potential variations in sleep. Subjects also will complete the Karolinska Sleepiness Scale. See the detailed schedule below.

Immunological and Inflammatory Responses At least 1 ml of passive drool saliva will be collected in the morning to determine the reactivation pattern of latent herpesviruses (a clinically relevant biomarker for immune dysregulation) as well as salivary stress biomarkers. See the detailed data collection below.

Testing Schedule

Test Schedule Time Cognitive Performance

• ANAM

1. Familiarization session

2. 2 x at home before returning to altitude

3. 1 per day during LA test days 1, 3, 5

4. 1 per day during MA test days 1, 3, 5, 14, 21, 28

60 min (Fam only) 30 min each for subsequent session

Symptomatology

• Lake Louise Score

One general visual analog scale (VAS) score for well-being (NASA-provided)

1. 1 per morning and 1 per evening at LA test days 5, 6, 7

2. 1 per morning and 1 per evening at MA test days1-7. 14, 21, 28

5 min each

Symptomatology

• Symptoms or diagnosis of

AMS, HAPE, HACE

1. As reported by study physician or investigator team

NA

Sleep Quality and Duration

• Actigraphy

1. Continuously during LA test days 1-7

2. Continuously during MA test days 1-28

NA

Sleep Quality

• Karolinska Sleepiness Scale

1. 1 per morning and 1 per evening at LA test days 5-7

2. 1 per morning and 1 per evening at MA test days 1-7, 14, 21, 28

5 min each

Immunological and Inflammatory Responses

• Saliva samples

1. 1 during LA at test day 7

2. 1 per day during MA at test days 7, 14, 21, 28 Provide cold storage (-20 °C) for the saliva samples and ship for analysis and cover analysis costs.

2 min each

The vendor will provide the personnel and coordinate the logistics to implement the test protocols, deliver the electronic data to NASA, provide cold storage (-20 °C) for the saliva samples, and coordinate shipment of the saliva samples to the laboratory for analysis. The vendor also will coordinate the return shipment of NASA hardware (actigraphy and devices).