SATPC0041494 Tab 04 4 SOW.pdf

Statement of Work for FTIR and UV/Vis-NIR Microscopes

Background:

The ICEE facility is a state-of-the-art planetary science research facility consisting of three separate stations: a Raman Microscope, an Ultra High Vacuum (UHV) chamber, and a Fourier Transform Infrared and Raman Experiments (i.e., FIRE) station. The ICEE Facility is a unique NASA resource capable of replicating the wide range of extraterrestrial environments needed for planetary science research. The purchases of an FTIR microscope and UV-Vis/NIR microscope that function in concert with the Raman microscope will establish a Planetary Science Molecular Spectroscopy and Spectrophotometry Center at NASA Ames. This center focuses on the molecular spectroscopy and spectrophotometry of biosignatures and biosignature diagenesis from both an experimental and theoretical perspective. This is an opportunity to further the understanding of biosignatures and biosignature diagenesis. Raman and FTIR spectroscopy are complementary techniques; a molecular vibrational mode occurs at the same wavelength in both IR and Raman spectra, but the intensity of a vibrational mode differs depending on the chemical bond.

Chemical bonds with a large dipole moment (e.g., O-H) exhibit strong IR intensities. In contrast, homonuclear bonds (e.g., C=C, C-C) or bonds with small electronegativity differences exhibit strong Raman intensity, making the FTIR and Raman combination an excellent tool for analysis in biosignature detection, since biosignatures are composed of carbon, hydrogen and oxygen. UV-Vis/NIR spectrophotometry is highly sensitive and widely used in planetary science, focusing on electronic transitions versus molecular vibrations. Correlating data from these three techniques on very heterogenous samples presents a unique and exciting opportunity.

The ICEE facility currently has a JASCO Raman microscope instrument with 405, 532, and 785 nm excitation lasers. This purchase request is for the selection of an FTIR and UV-Vis/NIR microscopes that all the measurement (single point and mapping) of the same locations on the UV-Vis to NIR to Mid-IR AND the Raman instruments! Providing complementary data on the same spot in a sample, whether it is an analog field sample, returned sample, lunar regolith sample, etc. would provide greater clarification regarding the organics (e.g., biosignatures) in the sample.

Scope and Specifications:

The FTIR microscope and UV-Visible/NIR Microspectrophotometer microscope instrument purchase shall include the following:

1. A mid-IR configuration infrared spectrometer with diode laser, KBr beamsplitter and high-Intensity ceramic IR Source with a

• Sealed and desiccated aluminum 28- degree Michelson interferometer

• The mid-IR instrument shall have an infrared spectral range from 7800-350 cm-1 or better with manual or automated beamsplitter and/or interferometer window exchange and capable of expansion to 25,000 to 10 cm-1.

• Spectral (not pixel) resolution 0.07 cm-1 or better in the mid-infrared.

• SN ratio of at least 55,000:1 at 4 cm-1 resolution, 1 minute collection time, near 2,200 cm-1.

• Inert gas purge (options full or partial vacuum)

• Auto accessory recognition

• Transmission cell holder

• Software capable of routine research data analysis such as peak height (ratio), peak areas (ratios), smoothing, spectrum conversion, wavelength conversation, curve fitting and deconvolution as well as export of data in universal formats as well as control, imaging and data analysis.

• Broadband KBr beamsplitter

• Connecting optics unit for an FTIR microscope

2. The Infrared Microscope shall attach to the IR spectrometer and shall have a

• Research grade FTIR Microscope with mid-band LN2 cooled MCT detector (standard) spectral range of 7800 to 550 cm-1.

• Automated XYZ stage. Movable distance X: 100 mm; Y: 75 mm; Z: 30mm (1 micrometer step). Maximum load: 5kg (max); 1 kg (std). Max 40 mm thick sample.

• Autofocus

• 16x Cassegrain objective and condenser pair (AI) for transmission/reflection measurements

• Automatic 4-position objective revolver (for optional ATR, 10x, 32x or grazing-angle Cassegrain objectives and 4x or 10x refractive objectives for observation)

• A diamond ATR microscope objective capable of single point contact mapping, and the capability of both observation of the sample and spectral measurement using a single objective.

• Pressure sensor for ATR measurement.

• IQ Mapping for single point, multipoint, line and grid mapping

• IQ Monitor for control and video image display of the aperture size and orientation;

automatic capture and simultaneous display of the sample image during spectral collection with auto-illumination, auto-diffraction compensation and auto-focus

• High-resolution CCD color video camera with 5 MP

• Option for additional, automatically selectable detectors (DLaTGS, IQDuo and InGaAs)

• IR Advanced search NAV, Advanced Spectra Search (ADSS) and Particle software analysis are included

3. Joystick for microscope movement.

4. IGA-5X InGaAs detector.

5. The FTIR microscope shall have a Purge case for the sample compartment.

6. The FTIR microscope shall have a sample holder frame that enables accurate sample re-positioning for sample re-analysis as well as ensures accurate sample position between the FTIR microscope, the UV/Vis NIR microscope and either our existing Raman microscope or a new Raman microscope. This holder shall include the holder, plate, and calibration sample/software

7. The purchase shall include a sample holder frame update for our existing Raman microscope, enabling accurate sample positions for repositioning and transfer of samples between the UV/Vis NIR, FTIR, and Raman microscopes, or a new Raman microscope capable of accurate sample repositioning and transfer of positions between the FTIR and UV/Vis NIR microscopes.

8. The UV/Vis NIR Micro-Spectrophotometer shall have a range or 200 nm to 1.6 um using a 10x Cassegrains objective, as well as

• Integrated dual-beam, dual-grating scanning monochromator system with halogen and deuterium sources (aircooled Xe source option)

• PMT and InGaAs detectors to provide a spectral range of 200nm to 1600 nm

• 16x transmittance and reflectance Cassegrains objective.

• Computer controlled 4-position revolver that can be fitted with any combination of a 10x, 16x and 32x cassegrain or 10x and 20x refractive objectives

• An automated XYZ auto-stage, working range of 76 x 52 mm for automated multi-point or mapping experiments

• Spectra Manager software for data measurement and analysis.

• Computer controlled circular and rectangular sampling apertures

• Minimum spatial resolution of 16 microns (circular aperture) or a rectangular aperture of 16 x 16 microns

• High resolution CCD color video camera for visual examination and sample positioning; the microscope aperture size and orientation is displayed on the video image

• video capture optical images are automatically saved with the spectral data.

9. Joystick for moving and focusing the sample.

10. A holder and frame enabling accurate repositioning of the sample and accurate position transfer between the UV/Vis-NIR microscope, the FTIR microscope, and our existing Raman microscope or a new Raman microscope.

11. Dell Pro Slim Desktop to run the two instruments and for data analysis.

• Intel Core Ultra 5 235 Processor

• 16 GB RAM

• 256 GB Solid State Drive

• Wireless Keyboard & Mouse

• Intel DDR5 SDRAM, DVD-Writer

• Windows 11 Professional, 64-bit, English, Factory Install

• Monitor not included - select from 22" or 24" monitor as required

• ProSupport 3-Year Service Plan -Next Business Day Parts and Labor On-Site Response (provided by Dell) 24/7 Tech support

12. Two (2) Dell 24" Monitors, one for each instrument (FTIR microscope & bench and the UV/Vis NIR microscope.

13. Installation & Training includes instrument and software familiarization

• Performed by a factory trained Service Engineers from the vendor.

14. A Silicon reference sample for Raman instruments with a pre-mounted silicon sample with polypropylene polymer standard in a black-coated metal slide shall be included with the purchase.

15. The UV/Vis-NIR Microspectrophotometer shall come with a MCAN-581 Color Diagnosis Analysis Program.

Period of Performance:

The period of performance shall be from award to 11/01/2026.

Government Cost Estimate:

The estimated cost for such a system should be $286,581.75.

NASA POC:

The NASA POC for this purchase is Dr. Andrew Mattioda, phone 650-604-1075; e-mail andrew.mattioda@nasa.gov Vendor POC:

A potential vendor POC is Mr. Kevin Taylor, JASCO, phone 909-957-0491; e-mail ktaylor@jascoinc.com; website: jascoinc.com Market Research:

An internet search was conducted to find a UV/Vis NIR microscope and FTIR microscope with the capabilities listed above, including the requirement to ensure accurate repositioning of the mailto:andrew.mattioda@nasa.gov mailto:ktaylor@jascoinc.com sample as well as the ability to accurately position the sample between the UV/Vis NIR and FTIR microscopes as well as our existing JASCO Raman microscope or a new Raman microscope with identical capabilities to our existing model. While multiple vendors exist for either UV/Vis NIR microscopes or FTIR microscopes or Raman microscopes, very few exist that do all three instruments, which is required to ensure accurate sample positioning between measurements/instruments. JASCO has an FTIR microscope as well as a UV/Vis microspectrophotometer, which complements our existing JASCO Raman microscope. JASCO has an iQ frame system permitting accurate repositioning of a sample on the instrument as well as accurate sample positioning between the Raman, FTIR and UV/Vis NIR microscopes. JASCO also has the ability to do micro-ATR measurements. CRAIC technologies has both a Raman and UV/Vis NIR microscope but its website does not list an FTIR or IR microscope or the capabilities to accurately reproduce sample positions between measurements or instruments.

HORIBA has a Standard Microscope Spectroscopy (SMS) system that is capable of Raman, UV/Vis NIR, and partial IR measurements. Based on our research two vendors were contacted, JASCO and HORIBIA. The cost of a HORBIA system would be around $335K (their estimate), to include the Raman, UV/Vis NIR and partial IR with the ability accurately measure the same positions with all three techniques, beyond our budgeted amount. However, the HORIBIA system does not cover the entire mid-infrared range, does not have a sample purge compartment for the IR, nor does it have the ATR infrared measurement capabilities. We did not go into the spectral resolution of the IR or UV/Vis NIR systems. JASCO meets all the requirements and is within our budget and time limitations. Thus, this purchase request will be as a sole-source purchase. Our facility currently has a JASCO NRS5500 Raman microscope. This purchase is being conducted to permit FTIR, Raman and UV/Vis-NIR microscopy on the same sites within a sample, necessitating the ability to accurately position the sample between Raman, UV/Vis NIR, and FTIR or IR techniques. The JASCO iQ frame permits such a correlation between instruments. This purchase also includes an upgrade to the existing JASCO NRS5500 Raman microscope, permitting the use of the JASCO iQ frame. A different vendor would not be able to provide the upgrade of the existing Raman microscope to use the iQ frame or the ability to cross-correlate sample positioning between the instruments with the iQ frame. Selection of a different vendor would drive up costs and impact the scheduling of this purchase. The inability to accurately reposition samples between instruments or collect the various measurements on the same sample location would negatively impact the science objective of cross correlating the different instrument data on very heterogenous samples.

Furthermore, there are a very limited number of UV/Vis-NIR microspectrophotometer suppliers available. Given the prevalence of infrared, UV/Vis-NIR, and now Raman measurements in planetary science, it would be beneficial to NASA to enhance competition in instruments combining multiple analytical techniques, such as Raman, IR, and UV/Vis NIR. This could be accomplished through either proposal calls or NASA’s SBIR program.