Tech-Snapshot-NanoFET-26-25007.pdf

Richard P. Feynman Center for Innovation

Technology Snapshot

Overview Infectious diseases and chronic conditions such as cancer continue to impose enormous public health and economic burdens worldwide. Conventional vaccines and biologics are typically designed against a single pathogen or target, leaving populations vulnerable when new or unknown threats emerge. For military personnel and first responders, the absence of broad-spectrum medical countermeasures means that exposure to an unidentified pathogen in the field can be met with little more than supportive care. In cancer immunotherapy, connecting tumor cells to the patient’s own immune effector cells remains a formidable engineering challenge; current bispecific antibody formats are complex to manufacture, expensive and often limited in the number of targets they can engage simultaneously. Meanwhile, traditional antibody-based therapeutics are large molecules that can be difficult to produce at scale, may trigger unwanted immune reactions and lack the modularity needed for rapid adaptation to new disease targets. A platform capable of addressing multiple threats through a single reconfigurable architecture would represent a meaningful shift in how therapeutics are developed and deployed.

Technology Description The NanoFET platform addresses these challenges through three complementary capabilities. First, nanobody-nanofiber chimeras (NNMs) can pair a pathogen-targeting nanobody with an immune-cell-engaging nanobody on the same nanofiber scaffold, physically bridging a disease agent to the immune cells best equipped to destroy it. One example is linking a pan-influenza nanobody to an anti-T-cell nanobody, or an anti-cancer nanobody to a T-cell activator.

Second, a dendritic cell-recruiting peptide conjugated to the Q11 nanofiber (QDP) activates innate immune responses in a pathogen-agnostic manner, offering a broad-spectrum countermeasure that does not require advance knowledge of the specific threat.

Third, the modular design allows high-density display of multiple functional components on a single nanofiber, enabling multiantigen vaccine configurations that can stimulate both innate and adaptive immunity simultaneously. Data confirms that QDP nanofibers are specifically internalized by dendritic cells and activate them, and that nanobody-nanofiber chimeras retain the binding functionality of the nanobody component as demonstrated by ELISA recognition of target proteins.

Because the methodology is compatible with other small protein biologics — including AI-designed binders developed by external organizations — NanoFET can serve as a universal chassis onto which diverse targeting molecules are loaded, dramatically reducing the time and cost of developing new therapeutics against emerging threats.

Advantages

• Modular architecture allows rapid swapping of nanobodies and peptides to address new disease targets without redesigning the core platform

NanoFET NanoFiber Engineering Therapeutics Platform

Value Proposition NanoFiber Engineered Therapeutics Platform (NanoFET) offers pharmaceutical and biodefense organizations a modular, adaptable scaffold for building next-generation immunotherapies. By merging self-assembling peptide nanofibers with interchangeable nanobody and peptide components, the platform enables rapid development of targeted treatments that bridge disease agents directly to the patient’s own immune cells.

Technology Readiness Level 3

IP Information for S-194657 U.S. Patent pending

Contact Information licensing@lanl.gov

An Equal Opportunity Employer / Managed by Triad National Security, LLC, for the U.S. Department of Energy’s NNSA

LA-UR-26-25007

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Advantages continued

• Dual-function capability bridges disease agents directly to immune cells on a single construct, enabling both targeted and broad-spectrum responses

• Adjuvant-free immune activation through self-assembling nanofibers that inherently stimulate robust, innate immune responses

• Small, stable targeting molecules (nanobodies) that are easier to produce and engineer than conventional full-size antibodies

• High-density multivalent display presents multiple antigens or functional components simultaneously, enhancing immune recognition

• Compatibility with external biologics enables partners to integrate their own AI-designed binders or proprietary targeting molecules onto the nanofiber scaffold

Market Applications

• Oncology (bispecific T-cell engagers, tumor-targeted immunotherapies, combination immunotherapy platforms)

• Infectious Disease Therapeutics (pan-influenza treatments, broad-spectrum antiviral and antibacterial countermeasures, emerging pathogen response)

• Biodefense and Military Medicine (medical countermeasures for warfighters, rapid-response therapeutics for unknown biological threats, field-deployable immune enhancers)

• Vaccine Development (multiantigen vaccine platforms, adjuvant-free subunit vaccines, mucosal and systemic immunization)

• Pharmaceutical Biologics and Drug Delivery (nanobody-drug conjugate scaffolds, targeted immune cell delivery, modular biologic platforms)

LA-UR-26-25007