1-Brand Name Justification.docx

· VI. Identification of the statutory authority permitting other than full and open competition 41 SC 253(c)(1) and FAR Part 13.106-1(b): Brand Name

· The Chromium kits and reagents are specific to the 10x Chromium Controller available for our use; no other kits/reagents are compatible with this instrument, and attempting to run third party reagents would not be supported by the instrument manufacturer. The 10x Chromium controller system requires proprietary reagents and consumables that can only be provide by the manufacturer, 10x Genomics, and two distributors. The Chromium Next GEM Reagent Kits is the only kits that enables deep profiling of complex cell populations with high-throughput 3’ digital gene expression and feature analysis of individual cells. Single-cell Sequencing with the 10X platform. There is only one source of products for library preparation which are available for CITE-seq analysis supplied by 10x Genomics. The technical protocol of 10X Genomics library preparation kit use in CITE-seq analysis was well established and shown in several reports. These patented, high-quality kits can generate single-cell transcript counts from up to 100,000 cell per run with high efficiency. Only 10X Genomics products will meet our needs. 10X Genomics patented, high quality kits are the ONLY kits that can generate single-cell transcript counts from up to 100,000 cells per run with high efficiency from a very small input of RNA. To ensure continuity of on-going project within NIAID, it is imperative to use these kits. Switching to an alternative product could invalidate the data results.