RFQ Attachment 1 - Statement of Needs.docx

Statement of Needs – CytoFLEX S Flow Cytometer & CytoFLEX SRT Cell Sorter

This statement of work is for the procurement of two instruments capable of: a) detecting and measuring traits of cell and virus populations, and b) separating cells and virus subpopulations from a sample based on their properties. These are our specific requirements:

Flow Cytometer

· Must have 4 lasers, including a violet (405 nm) laser for small (<100 nm) particle detection and 3 additional lasers (488, 561, and 638 nm) for fluorescence applications.

· Must have Violet Side Scatter Resolution (VSSC) and the capability to detect 80 nm polystyrene particles.

· Fluorescence resolution: the instrument can achieve <3% rCV.

· Sensitivity parameters:

-FITC: <30 molecules of equivalent soluble fluorochrome (MESF‐FITC) from the 488 nm laser -PE: <10 molecules of equivalent soluble fluorochrome (MESF‐PE) from the 488 nm laser (this property is essential for detecting small particles, such as viruses)

· Fixed integrated optics and quartz flow cell design with >1.3 numerical aperture.

· Proprietary fluorescence and side scatter light delivery by fiber optics design to ensure high performance, high efficiency, and low-noise signal detection.

· Must have adjustable flow rate options that range from 10 to 240 µl/min to provide high sensitivity (in both small particle and low concentration regimes)

· Must have plate loader options that can accommodate standard and deep well 96 well plates.

· Must have compact size, up to 17x17x13.5 in. Must include 10 L Sheath and Waste tank options.

Cell Sorter

· Must have 4 lasers, including a violet (405 nm) laser for small (≤200 nm) particle detection and 3 additional lasers (488, 561, and 638 nm) for fluorescence applications.

· Must have Violet Side Scatter Resolution (VSSC) and the capability to detect particles ≤200 nm.

· Fluorescence resolution: the instrument can achieve <3% rCV.

· Must be capable of processing samples at a range of flow rates between approximately 10 μL and 100 μL per minute providing high sensitivity (in both small particle and low concentration regimes).

· Must have increased accuracy of population identification and high sensitivity to measure events with low staining index for rare event detection and application with dim fluorescent staining. Sensitivity parameters:

-FITC: <30 molecules of equivalent soluble fluorochrome (MESF‐FITC) from the 488 nm laser -PE: <10 molecules of equivalent soluble fluorochrome (MESF‐PE) from the 561 nm laser -APC: <25 molecules of equivalent soluble fluorochrome (MESF-APC) from the 638 nm laser

· Fixed integrated optics and quartz flow cell design.

· Accepts the following collection devices: 6-, 24-, 48-, 96- and 384-well plates, 96-deep well plate.

· Must be able to perform system startup, QC and sort calibration to be ready to sort within 30 minutes. All sort settings can be defined automatically.

· Must be able to generate 30,000 Hz to 35,000 Hz droplets and optimize the settings automatically for sorting.

· Must have technology used for automatic droplet optimization, monitor and maintain, and drop delay determination.

· Must be capable of simultaneously bulk sorting up to 4 defined populations of particles >99% purity with 5% target population, sample threshold ≤10,000 events per second, while the yield rate is above 80% theoretic rate.

· Must have up to four-way sorting with mixed modes to 5 mL and 15 mL tubes; one-way sorting can be conducted with up to 96 deep well plates and standard 384 micro-well plates or plates.

· Must have compact size, up to 29x19x18 in.