RFQ Attachment 1_Purchase Description.pdf

FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The purpose of this acquisition is to provide supplies (cDNA-PCR long read sequencing kits and PromethION Flow Cells) to the Center for Alzheimer’s Disease and Related Dementias (CARD) for neurodegenerative disease research for the purpose of long read sequencing on Oxford Nanopore manufactured sequencing systems. These reagents will be used to identify causative genes for neurodegenerative diseases.

2. Background Information and Objective:

The National Institutes of Health (NIH) Intramural Center for Alzheimer’s and Related Dementias (CARD) is a collaborative initiative of the National Institute on Aging (NIA) and National Institute of Neurological Disorders and Stroke (NINDS) that supports basic, translational, and clinical research on Alzheimer’s disease and related dementias.

CARD’s central mission is to initiate, stimulate, accelerate, and support research that will lead to the development of improved treatments and preventions for these diseases. Understanding the molecular signature of human brain is essential to study pathogenesis of neurodegenerative diseases. High-throughput cDNA-PCR sequencing will assist in epigenetic analysis in post-mortem frontal cortex tissue. The goal is to identify potential therapeutical targets and biomarkers in patients with early-onsite and late-onsite Alzheimer’s disease.

3. Generic Name of Product: cDNA-PCR long read sequencing kits and PromethION Flow Cells

4. Purchase Description: cDNA-PCR Sequencing Kits for long-read sequencing.

5. Salient characteristics/Project Requirements:

The Oxford Nanopore PromethION device is an electronic analysis system for use in scientific research. The core technology is built around a nanopore that can detect single molecule events including nucleic acids (DNA/RNA), proteins and small molecules. PromethION device is currently used by the CARD researchers.

The required consumables are used with PromethION devices that enable high-output, high-throughput sequencing of DNA or RNA samples. The flow cell kits are designed to allow pooling and running multiple libraries on Oxford Nanopore devices. It allows sequential runs of multiple sequencing libraries in the same flow cell. All reagents must be compatible with, and optimally perform in the Oxford Nanopore manufactured systems.

Salient characteristics include: The flow cell must be able to generate up to 290 Gb for sequencing DNA, cDNA or native RNA in real-time; the flow cell must be compatible with Nanopore PromethION 48 sequencers in form, fit, and function; The flow cell must contain Application- Specific Integrated Circuit (ASIC) to control and measure sequencing process; and the flow cell must contain recyclable electrical components.

FAR Part 11—Describing Agency Needs

6. Quantity:

PromethION Flow Cells v 10.4.1 (Pack of 4) – Cat. No. FLO-PRO114 – QTY = 25 Sequencing Kit, cDNA-PCR Barcoding v Kit 114 – Cat. No. SQK-PCS114 – QTY = 34

Optional QTY 1 PromethION Flow Cells v 10.4.1 (Pack of 4) – Cat. No. FLO-PRO114 – QTY = 25 Sequencing Kit, cDNA-PCR Barcoding v Kit 114 – Cat. No. SQK-PCS114 – QTY = 34

Optional QTY 2 PromethION Flow Cells v 10.4.1 (Pack of 4) – Cat. No. FLO-PRO114 – QTY = 25 Sequencing Kit, cDNA-PCR Barcoding v Kit 114 – Cat. No. SQK-PCS114 – QTY = 34

7. Delivery: Delivery will be f.o.b. destination. Delivery shall be made to the National Institute on Aging located at 251 Bayview BLVD, Baltimore, MD 21224 The anticipated delivery dates are shown below.

Description Catalog No.

Quantity Optional QTY1 Optional QTY2

08/20/2025 08/20/2026 08/20/2027

PromethION Flow Cells, v10.4.1 (4 pack) FLO-PRO114M 25 25 25 cDNA-PCR Sequencing kit v14 SQK-PCS114 34 34 34