RFQ 75N91024Q00085 - SON.pdf

STATEMENT OF NEED (SON)

75N91024Q00085 Page 1 of 5 National Institutes of Health / National Cancer Institute / Office of Acquisitions

1.0 TITLE

Procurement of one (1) Brand-Name or Equal Revvity Envision Nexus Multimodal Plate Reader and Associated Instrumentation

2.0 BACKGROUND

The Urologic Oncology Branch (UOB), within the Center for Cancer Research (CCR), at the National Cancer Institute (NCI) is focused on the study of hereditary urologic cancer at the clinical, genetic and molecular levels. UOB performs clinical research contributing to the development of surgical and diagnostic procedures, and therapeutics against human Genitourinary Cancer. UOB has a clinical surgery patient tumor sample bank, tumor cell lines, blood or other samples in a branch repository. UOB is also engaged in translational research via clinical trials. In support of this translational research, UOB is collaborating with the National Center for Advancing Translational Sciences (NCATS) to screen small molecules inhibitors and perform combination matrix screens against several types of kidney cancers as well as bladder cancer. Research Toward a Cure is part of an ongoing effort by UOB researchers to develop tools that enhance scientists' technical capabilities and advance cancer research.

In support of this preclinical cancer research, the UOB requires one (1) Brand-Name or Equal Revvity Envision Nexus Multimode Plate Reader and associated instrumentation. This equipment will used for experiments to include protein estimation analysis, enzyme kinetic assays, competition binding experiments, viability cell death dual florescent assays, cytotoxicity assays, drug screening/ drug binding assays/FRET, NADPH/NADP and NADH/NAD detection assays, metabolic assays (glucose uptake, lactate secretion, ATP assays, colorimetric ROS measurement assays, ELISAs/EIAs, multiple caspase activation assays, Immunoassays, functional Kinase assay (Ca release, cAMP and cGMP measurement, B-arresting assays), drug dissolution profiles, and enzyme kinetics (e.g., Ki, Km, etc.).

3.0 TYPE OF ORDER

This shall be a Firm Fixed-Price Purchase Order.

4.0 SPECIAL ORDER REQUIREMENTS

4.1 PRODUCT FEATURES/SALIENT CHARACTERISTICS

The following product features/characteristics are required for this requirement:

• Occupy a space no larger than 50cm W x 60cm D x 50cm H

• Must be able to conduct automatic data analysis.

• Shall provide the end user with reference materials, user guides, on how to support quality control and verify system functions and standardization settings (hardware configuration, laser power, gain settings, fluid stream).

http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&sqi=2&ved=0CCYQFjAA&url=http%3A%2F%2Fwww.ncats.nih.gov%2F&ei=NO9fU4qDC6qGyQGjzoDwDA&usg=AFQjCNFs_tihE0B-mczYYky6B-d0BQdTjA&bvm=bv.65397613,d.cWc http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&sqi=2&ved=0CCYQFjAA&url=http%3A%2F%2Fwww.ncats.nih.gov%2F&ei=NO9fU4qDC6qGyQGjzoDwDA&usg=AFQjCNFs_tihE0B-mczYYky6B-d0BQdTjA&bvm=bv.65397613,d.cWc

75N91024Q00085 Page 2 of 5

• Be capable of both filter and monochromator-based detection for absorbance and fluorescence intensity.

• Be modular and upgradable.

• Include two (2) Excitation Monochromators and two (2) Emission Monochromators for dial down absorbance and fluorescence assay top reads

• Must be a multi-mode instrument equipped with, at a minimum, absorbance, florescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence (TRF) plate reading capability.

• Must be capable of top and bottom plate reading for luminescence, florescence intensity, and time-resolved fluorescence (TRF).

• Must be capable of Ultraviolet (UV) and Visible absorption detection.

• Must include, at a minimum, amplified luminescent proximity homogeneous assay (Alpha) technology, for high throughput screening (HTS).

• Must be able to process a variety of cell types, to include blood/immune cells, stem cells / stem cell colonies, and tumor spheroids.

• Must be capable of ultrasensitive luminescence with dedicated photomultiplier tube (PMT) detector to facilitate a wide variety of highly sensitive immunoassays, such as AlphaScreen/AlphaLISA assay, Time-Resolved FRET assays, Time-Resolved Fluorescence (TRF) Loop-mediated isothermal amplification (LAMP) assays for the detection of low-expressing samples.

• Include a user-friendly workstation interface.

• Include computer and monitor for workstation interface. The computer operating system shall be Windows 10 (or higher).

• The computer shall be equipped with brand name or equal Kaleido 4.0 Software, which combines data acquisition and image analysis with a workflow-based user interface that offers complete imaging control for the multi-mode plate reader. The software shall be intuitive, user-friendly and capable of quantitative and qualitative analysis, comparative analysis, and produce high-quality data plot images. The software shall have the capability to set up individual user accounts with customized roles and setting permissions. With CAN-card connection to SQL Server Database the software shall contain up to 10GB of storage.

• Include Robotic Microplate Stackers for 20-plate magazines.

• Include barcode reader for reading slides labeled with barcodes.

75N91024Q00085 Page 3 of 5

• At a minimum, shall accommodate 6, 24, 96, 384, and 1536-well microplates.

• Must include two (2) channel reagent dispensers with dual tip mounts for performing timed injection of reagents.

• Must have temperature control range of 20-60 degrees Celsius.

Additional Requirements

• Must include a minimum of 12-month manufacturer’s warranty.

The following product fulfills this requirement:

(1) Revvity EnVision Nexus Multimode Plate Reader, inclusive of the following:

Item # Item Description Product No Quantity

1 EnVision Nexus 2-detector HH36000002 1 2 EnVision Nexus 2detector base instrument

HH36000052 1

3 Country Kit US/CAN HH36000100 1 4 Ultra-sensitive luminescence module HH36000200 1 5 Enhanced Alpha Module HH36000220 1 6 TRF laser module HH36000230 1 7 Stacker HH36000250 1 8 Barcode Reader Upgrade HH36000410 1 9 laser collimation block HH36000225 1 10 Stacker magazines - 20 plates HH36000251 1

4.2 WARRANTY

In addition to the Implied warranty contained in FAR Clause 52.212-4, the Contractor is encouraged to provide express warranties. At a minimum, the Contractor shall provide a 12-month warranty from date of final installation on all equipment and associated items provided under, or as a part of this purchase order. The warranty shall include repair and or replacement of parts, including travel, labor, and materials, at no additional cost to the Government. The warranty shall not exclude or limit the Governments rights for an Implied warranty as established in the terms and conditions of clause 52.212-4.

4.3 DELIVERY / INSTALLATION

Delivery shall be within four (4) weeks of the purchase order award. All shipping/handling (including FOB Destination) and delivery/installation fees shall be included in the quote. Upon delivery, the Contractor shall notify the respective NCI Technical Point of Contact to schedule installation dates and times. Installation shall occur within fourteen (14) calendar days of delivery and shall be performed by, or under the direct supervision of, a certified operator.

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The contractor shall deliver and install the equipment at the following address:

National Institutes of Health/NCI 9000 Rockville Pike Bethesda, MD 20892 Technical Point of Contact: [to be provided upon award]

4.3.1 DELIVERY GUIDANCE

The Contractors shall communicate and follow instructions from the Technical Point of Contact (TPOC) to coordinate delivery and installation.

The Contractor shall direct all courier and delivery services – including those not operated by the Contractor (e.g. FedEx, USPS, UPS) – to follow the instructions in this section.

All companies delivering to the NIH main campus must comply with Commercial Vehicle Inspection Facility (CVIF) access procedures. Upon arrival, the CVIF will provide a list of buildings and loading docks to delivery personnel.

Deliveries will be logged in at the loading dock, and the loading dock contractor or Government personnel will attempt to contact the TPOC listed on the package. In the event that the TPOC is unable to accept the package, the delivery shall not be left at the loading dock station. The delivery must be returned to the Contractor, and the Contractor must coordinate with the TPOC to deliver on another date at no additional cost.

Deliveries can only be made between 6:00 a.m. and 4:00 p.m. EST, Monday – Friday, excluding federal holidays.

4.4 INSPECTION AND ACCEPTANCE

Pursuant to FAR Clause 52.212-4, all items to be delivered in relation to this Purchase Order are subject to final inspection and acceptance by an authorized representative of the Government.

The authorized representative of the Government is the TPOC who is responsible for inspection and acceptance of all services, materials, or supplies to be provided by the Contractor.

The TPOC has limited authority as defined within this Statement of Need. They do not have the authority to commit or obligate Government funds or make changes to the terms and conditions of the purchase order. As such, any changes to the purchase order must be performed by a warranted Government Contracting Officer with appropriate delegated authority to do so.

4.5 TRAINING

The Contractor shall provide on-site training for up to 10 users. Training shall be conducted by a certified operator. The user training shall include review and demonstration of all features and functionality of equipment and software. Training shall include an overview of setup approaches, maintaining instrument settings, establishing operational voltages, and software usage for custom instrument configurations, as well as a demonstration of the performance.

Instrument care and maintenance must also be covered. The training shall be provided at a time specified by the Technical Point of Contact, within fourteen (14) days following installation of

75N91024Q00085 Page 5 of 5 the device. All training expenses, including materials and or travel expenses, shall be included in the quoted price.

5.0 PAYMENT

Payment shall be made in full after the successful delivery, installation, training and acceptance of equipment by the NCI Technical Point of Contact (TPOC). Payment authorization requires submission and approval of invoices to the NCI TPOC and NIH OFM.