Requirements 2033056.pdf

Requirements for microscope

To be considered for award, vendors of "equal" products, including "equal" products of the brand-name manufacturer, must meet the following criteria for the salient characteristics of the Microscope.

• Microscope for bioluminescence and fluorescence imaging of human erythrocytes o 100x oil objective for visualization of small cells with both bioluminescence and fluorescence o Fluorescence excitation filters at 470 nm and 505 nm with emission filters at 525 nm and

605 nm

• Small enclosure for efficient shielding of sample and optics from external light with maximum external dimensions of 24 inches x 24 inches, housed on a vibration isolation table with dimensions of 30” x 30“

• Incubation chamber for long-duration imaging with temperature, humidity, and gas control to permit observation of healthy cultured cells through the 48 hour lifecycle of the malaria parasite P. falciparum

• Flexible and automated software (included in the quotation) to permit long-term, time-lapse acquisition of luminescence and fluorescence data from cultured erythrocytes with robust capability for image analysis

• Manual stage control for X-Y and Z focus.