Purchase description Single Cell Dispenser.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: This acquisition is necessary to provide a single cell dispenser with multichannel fluorescence and high-resolution imager to the iPSC Neurodegenerative Diseases Initiative (INDI) Project within the new Center for Alzheimer’s Disease and Related Dementias.

2. Background Information and Objective: imaging technology improves to capture The single cell dispensing method is based on a unique microfluidics-based print technology that is as gentle as pipetting. The cell-containing microdroplets are dispensed slowly and delicately in the absence of any pressure, electric fields, or lasers observed in other technologies. The unique ability to screen cell populations by gating for size and roundness offers the potential to exclude imperfect non-viable cells that would or can cause inconsistent data and variances that would affect the research work related to the pathogenesis of neurodegenerative diseases. The multichannel imager software analysis determines accurately the confluence of each well, eliminating user subjectivity.

3. Generic Name of Product: Single cell dispenser with multichannel fluorescence and high-resolution imager

4. Purchase Description:

Single-Cell Printer™ F.SIGHT system includes single cell printing head with P9 piezoelectric dispensing unit, agitation adapter, 8-bit CCD camera, 32-bit RGB camera, fluorescence detection and fully configured embedded computer with dedicated imaging software pre-installed.

Microfluidics-based, disposable single-cell dispensing cartridge. Comes individually packaged and sterilized, 10 cartridges per box

Imager with Multichannel Fluorescence and High-Resolution Imaging.

CSI instrument installation, onsite-

Training, onsite, one day

Salient characteristics All deliverables required under this contract shall be packaged, marked and shipped in accordance with Government specifications. At a minimum, all deliverables shall be marked with the contract number and Contractor name. The Contractor shall guarantee that all required materials shall be delivered in immediate usable and acceptable condition.

Vendor must be able to provide these specifications:

Disposable Micro-Fluidics ChipTechnology Cartridge dimensions (mm) - ca. 8.8 (W) x ca. 16 (H) x ca. 5 (D) Printing Nozel Dimensions - ca. 40 mm by 40 mm Chip Material - Glass and silicon Disposable Cartridge Volumes - 5–80 μL Cartridge Throughput – 10 (96 well SBS format plates) Cartridge Sterility - UV sterilized, TSE/BSE-free Axis system and precision. Open three-axis Gantry system with ca. 100 mm precision or less (not including backlash); print-head mounted on z-axis, which includes optics, dispenser, and agitation.

Optical System Magnification and Numerical Aperture F.Sight - 10x mag (0.14 NA)with 90° beam deflector, brightfield, c-mount

Cell Camera F.Sight - 8-Bit grayscale 1280x1024 (bright field camera) and 1936x1216 (fluorescence camera) Droplet Camera 32-bit RGB, 1280–1024 pixels

Light Sources LED source 1: white, 1 watt LED source 2: red, 3 watts

Fluorescence Detection

Excitation: 473 nm Emission: 525/20 nm Laser power: 1- 100 mw (freely tunable) Exposure times: 0.1 – _20 ms (freely tunable)

Vacuum Manifold

Ensures that no doublets or zero cells are deposited during depositing, thus prevent # of wrong cells accidentally being deposited.

Microplate compatibility

SBS or SLAS standard cell culture well plates (96- and 384-well format May be customizable with additional formats (6-well to 24-well, tubes

Instrument sterility Resistant against 70% Ethanol, Resistant against UV (254 nm CWL)

Component sterility Metal piston guide, tubing (agitation and shutter), and luer-lock connectors are Resistant to ethanol and isopropanol. Piston guide and tubing can be autoclave (121ºC) Sterile filters must support luer-Lock in and out connections. Pore sizes can be 0.2 μm, 0.45 μm,

0.8 μm, or 1.2 μm

Data Analysis.

Each cell printed has recorded specifications for gating characteristics such as size, shape, roundness.

4. Quantity 1:

Single-Cell Printer™ F.SIGHT system includes single cell printing head with P9 piezoelectric dispensing unit, agitation adapter, 8-bit CCD camera, 32-bit RGB camera, fluorescence detection and fully configured embedded computer with dedicated imaging software pre-installed. 1 year warranty covering parts and labor. Installation kit included. Microfluidics-based, disposable single-cell dispensing cartridge individually packaged and sterilized, 10 cartridges per box QTY 1, Imager with Multichannel Fluorescence and High-Resolution Imaging. Imager Remote Results Viewer. (includes 5 licenses) QTY 1, CSI INSTRUMENT INSTALLATION, ONSITE. QTY 1

INSTRUMENT TRAINING, ONSITE, QTY ONE DAY

5. Delivery Date: Delivery date of 2 months after receipt of completed purchase order.

6. Period of Performance: Manufacturer warranty for one calendar year beginning on date of receipt.