Purchase Description_SAM.gov.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: Dr. Acosta-Rodriguez’s Circadian Biology of Aging Unit (CBAU) focuses on analyzing mRNA expression levels associated with cellular senescence, aging, and lifestyle interventions, emphasizing the influence of time and time of day. Our research frequently relies on large-scale real-time quantitative PCR to measure and quantify RNA expression, with sampling occurring every 4 hours over 2 days, in triplicates.

These analyses necessitate precisely calibrated and well-maintained instruments to ensure accuracy and reliability, especially when handling precious samples. Acquiring an updated real-time PCR machine, fully supported by the vendor with preventive maintenance agreements and calibrations, is essential for our research needs.

2. Background Information and Objective:

Real-time quantitative PCR (qPCR) machines are essential tools in circadian and aging research due to their precision in quantifying gene expression levels, including those linked to senescent cells. Understanding the decline of circadian rhythms with aging is vital for elucidating their role in chronic diseases and comorbidities in mammals. qPCR allows researchers to measure the expression of specific genes related to lifestyle interventions (such as jet lag, diets, and exercise), aging, and cellular senescence, providing insights into the underlying molecular mechanisms.

Real-time qPCR offers precise and rapid quantification of gene expression, facilitating the detection of subtle changes in circadian markers within metabolic tissues, which possess their own circadian clocks. This quantitative approach is crucial for identifying and characterizing 24-hour rhythms in gene expression in both healthy and aged cells. It also enables the rapid assessment of how lifestyle interventions (such as reducing sedentarism, managing jet lag, and preventing overeating) can promote circadian function and provides mechanistic insights into the onset and development of age-related pathologies.

This real-time PCR instrument will be the first of its kind in the Circadian Biology of Aging Unit (CBAU) lab, enabling innovative research to identify genes associated with cellular senescence under circadian clock disruption. The new instrument must seamlessly integrate with existing systems, utilizing the same chemistries, consumable reagents, calibration plates, software analysis, and preventive maintenance service agreements to minimize experimental inconsistencies between wells and equipment.

3. Generic Name of Product: QuantStudio™ 7 Pro Real-Time PCR System (96-well) with computer.

4. Purchase Description:

HARDWARE:

● QS7-PRO 96W 0.2ML QPCR,LAPTOP (1 unit)

● STD 96-WELL 10-DYE CAL KIT96-WELL ROI & BACKGROUND PLATES (1 unit)

● TAQMAN RNASE P 96-WELL INSTR (1 unit)

● 96-WELL HARD SHELL PLATE CLEAR (1 unit)

● KIT,802.11AC/B/G/N WIFI DONGLE (1 unit)

● OPTICAL ADHESIVE COVERS (1 unit)

OTHERS:

● SMARTSTART QS 6/7 PRO 1DAY, CS TRAINING (1 unit)

● Shipping & Handling

5. Salient characteristics Crucial features for these real time PCR systems/ The Applied Biosystems QuantStudio 7 Pro Real-Time PCR System offers innovative smart features in a compact design, enhancing the qPCR workflow with high quality, high throughput, and excellent reliability. Key features include:

● Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). The PCR systems must be able to quantitate real time signals from two types of chemistries used to detect PCR products using Sequence Detection Systems (SDS) instruments: • TaqMan® chemistry (also known as “fluorogenic 5´ nuclease chemistry”) • SYBR ® Green I dye chemistry.

● Hands-Free Operation: Facial authentication and voice commands for easy access to personal profiles and settings.

● Smart qPCR Workflow: Embedded RFID readers for automated data tracking and reduced manual entries, using TaqMan Array Plates with RFID tags.

● Compact and Standalone: Small footprint with direct touchscreen operation for setup, monitoring, and analysis.

● Smart Help: Direct touchscreen access to Technical Support, minimizing downtime.

● Smart Remote Support: Immersive tool for quick issue resolution with Technical Support, reducing instrument downtime.

● Automation Compatibility: Interchangeable blocks and 24/7 operation capability for increased productivity.

● Cloud Connectivity: Connect platform for secure data storage, analysis, and sharing, with remote monitoring via the Instrument Connect app.

● Versatile Applications: Compatible with over 8 million TaqMan Assays for diverse experiments, including gene expression and genetic variation.

● Flexible Formats: Supports multiple block types and integration with the Orbitor Microplate Mover for high-throughput workflows.

● High Sensitivity and Accuracy: OptiFlex system with six decoupled filter channels for improved data accuracy and multiplexing.

The QuantStudio 7 Pro system is designed for ease-of-use, making it ideal for researchers seeking a smart, reliable, and versatile qPCR solution.

6. Quantity: A43169 QS7-PRO 96W 0.2ML QPCR,LAPTOP (1 unit); A26343 STD 96- WELL 10-DYE CAL KIT; 4432364 96-WELL ROI & BACKGROUND PLATES (1 unit); 4432382 TAQMAN RNASE P 96-WELL INSTR VERIF PLATE (1 unit); 4483354 96-WELL HARD SHELL PLATE CLEAR,20 PCS PK (1 unit); A26774 KIT,802.11AC/B/G/N WIFI DONGLE (1 unit);

4360954 OPTICAL ADHESIVE COVERS 25 PCS/PACK (1 unit); TRN00303 SMARTSTART QS 6/7 PRO 1DAY, CS TRAINING (1 unit); Shipping & Handling (1 unit).

7. Delivery Date: within 2-8 weeks ARO.

8. Period of Performance: This system should be operational and continue to provide quality data for a period of no less than 7 (seven) years from date of delivery.

9. Option(s): n/a