Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The purpose of this acquisition is to purchase the Chromium X.

2. Background Information and Objective: The Chromium X powered by next GEM technology, provides a precisely engineered reagent delivery method that enables thousands of micro-reactions in parallel. Encapsulate a sample into hundreds to tens of thousands of uniquely addressable partitions in minutes, each containing an identifying barcode for downsteam analysis. Each Gel Bead, infused with millions of barcoded oligonucleaotides, is mixed with a sample, which can be individual cells or nuclei. Gel beads and samples are then added to an oil-surfactant solution to create Gel Beads-in- Emulsion (GEMs), which act as individual reaction vesicles in which the Gel Beads are dissolved, and the sample is barcoded. Barcoded products are pooled for downstream reactions to create short-read sequencer compatible libraries. After sequencing the resulting barcoded short read sequences are fed into turnke analysis pipelines that use the barcode information to map reads back to their original single cell or single nucleus of origin. The Chromium X series enables internet connectivity to the Cloud.

3. Generic Name of Product: Chromium X

4. Purchase Description: Chromium X cat. # 1000386.

5. Salient characteristics:

a. Flexible, low to high throughput.

b. Easy to use, more interactive user interface.

c. Make large-scale studies more affordable and practical with high-throughput single cell gene expression.

d. Improved hardware robustness including additional sensors

6. Quantity: 1 instrument

7. Delivery Date: 30 days from the purchase date.