Purchase Description 8.2.22.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

1. Statement of Need and Purpose: The purpose of this equipment is to lyse bacteria for making recombinant proteins.The reasons for this order:

This equipment will be critical for the central mission of the Glial Cell Biology unit to study the cell biology of oligodendrocytes. NIH is requesting this item to study how protein structure and modifications affect aggregation in neurological disease.

2. Background Information and Objective: In the central nervous system, oligodendrocytes are cells responsible for producing myelin, the insulation around electrically active axons of neurons. Myelin facilitates fast electrical conductance in an analogous way as rubber insulation around electrical wiring. Proper development and maintenance of oligodendrocytes has important implications on demyelinating disease, including multiple sclerosis, a disease in which immune cells attack and destroy myelin, and leukodystrophies, childhood diseases in which myelin fails to form properly leading to progressive disability. In addition, recent studies also support a role for oligodendrocytes in psychiatric disease and in memory, including in Alzheimer’s disease.

Our lab focuses on understanding the cell biology and building blocks of the oligodendrocyte, and how they contribute to normal brain function and disease. The oligodendrocyte is a cell that is shaped like a tree, with many branches that terminate to form myelin sheaths, which wrap concentrically around the axon in many layers, much like a long paper towel roll or a bolt of fabric. Our questions primarily underly the structure of these branches – microtubules that form the roads of these cells – and the cargos that travel along them, including organelles and mRNA genetic messages.

NIH has recently discovered that protein aggregation in oligodendrocytes may underlie the cellular etiology of the neurological disease MSA. NIH plans to use this machine to purify the aggregating protein in this disease so that it can study its modifications and aggregating properties.

3. Generic Name of Product: High-pressuer homogenizer

4. Purchase Description:

(1) Trade name: High-pressure homogenizer

(2) Product’s brand name: Avestin Emulsiflex C3 EF-C3 (or equivalent per FAR 52.211-6

BRAND NAME OR EQUAL (AUG 1999)

Stainless Steel Cooling Coil C5HECOIL1 (or equivalent per FAR 52.211-6 BRAND

NAME OR EQUAL (AUG 1999)

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

5. Salient characteristics

The equipment/system shall contain the following:

• Two air inputs (internal air source or building pressurized air)

• Use of the Emulsiflex C3 lysis process to use pressure to break open bacterial cells so that recombinant proteins can be purified. An alternate process must contain data demonstrating equal or superior toxicity and user discomfort when compared to other methods of lysis.

• Minimum Capacity: 3 L/h

• Pressure: up to 30,000 psi (2,000 bar)

• Minimum Sample Volume: 10 mL

• Minimum Power: 115/230 VAC, 50/60 Hz, 1 hp (0.75 kW)

• Stainless Steel Cooling Coil

6. Quantity: 1 of each

7. Delivery Date: 4-6 weeks after receipt of the order.

8. Period of Performance: Indefinite.

9. Option(s):. N/A