PUR 7776 Single Source with AB Sciex, LLC.pdf

PUR 7776 (Revised 07/2022) Page 1 of 8 Incorporated by Reference: Rule 60A-1.045, F.A.C.

DESCRIPTION OF INTENDED

SINGLE SOURCE PURCHASE

(PUR 7776)

AGENCY: Department of Environmental Protection

TITLE: Single Source with AB Sciex, LLC Short description of the commodity or service desired: Purchase of a SCIEX Triple Quad 7500 System.

CONTACT

Name: Belinda Croft Address: 3800 Commonwealth Blvd., MS93, Tallahassee, Florida 32399 Telephone: 850.245.2467 Email: Belinda.Croft@FloridaDEP.gov

Internal tracking number, if any: 2023SS0006

Date posted: January 13, 2023 Last day for receipt of information: February 6, 2023

This description of commodities or contractual services intended for purchase from a single source is posted in accordance with Sections 120.57(3) and 287.057(3), Florida Statutes (F.S.), and will remain posted for a period of at least 15 business days.

Commodity or Contractual Service Required (commodity or United National Standard Products and Services Code (UNSPSC), manufacturer, model, and description, as appropriate):

73171605 Laboratory Equipment Manufacture Services 81101706 Laboratory Equipment Maintenance

Quantity or Term (as appropriate):

Approximately 12 months

Requestor (division, bureau, office, individual, as appropriate):

Division of Environmental Assessment and Restoration (DEAR), Bureau of Labs, Chemistry Program

Performance and/or Design Requirements (e.g. intended use, function or application, compatibility, requirements; reference to policy, rule, statute or other act of the Legislature, etc., as appropriate):

Purchase of a SCIEX Triple Quad 7500 System.

SCIEX Triple Quad 7500 System. An Enhanced high-performance triple quadrupole LC-MS/MS mass spectrometer. The instrument upgrade is expected to aid in achieving lower detection limits, provide improved turn-around times, and facilitate method performance improvements.

Quotation Sub Total: $430,795.32

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Less Trade-In Credit: - $100,000.00 (For return of Triple Quad 6500+ S/N CF20981606) Estimated Freight: $5,999.00 Quotation Total: $336,794.32*

Part Number: 5077299 - SCIEX Triple Quad 7500 System - QTRAP Ready ($393,052.72) Enhanced high performance triple quadrupole LC-MS/MS mass spectrometer is QTRAP Ready.

System can be upgraded to QTRAP with the QTRAP Upgrade License. This system offers a mass range of m/z 5 to 2000 in triple quadrupole mode. Includes Optiflow Pro Ion Source, Optiflow Pro ESI tower with analytical probe and E-Lens optimized for flow rates 200-1000 μL/min. Addition options available for alternate flow rates and ionization modes.

System includes integrated syringe pump and 6 port, 2 position switching valve. Two wet pumps (roughing pumps) included. System ships with Data Acquisition Computer running Windows 10 64 bit and standard monitor. SCIEX OS Software is required for operation and must be purchased separately. Instrument will not operate without the purchase of the SCIEX OS software.

Limited Warranty: Standard parts and labor warranty for one year starting from the completion of instrument commissioning {as provided in SCIEX's written limited warranty statement and accompanying terms in the user manual or other product documentation}. Includes our StatusScope Remote Monitoring Service and one no-charge Preventative Maintenance (PM) during the one-year warranty period.

Part Number: 5090233 - SCIEX OS Acq TQ Starter Kit - OS-MQ 2.2 ($27,527.91) This bundle contains; 1) SCIEX OS for Acqusition & Quant Proc License on single workstation

2) Sciex OS-MQ license for quantitation of small molecule compounds, large molecule biomarkers and biopharmaceuticals on SCIEX QQQ LC-MS systems.

Part Number: TRNLP003 - Success Master ($10,214.69)

• Overview: Your success means everything. This SCIEX Now Learning Hub course is our most powerful and effective learning program designed to meet your exact needs. It is personalized to your instrument, workflow and experience level. The training process provides a unique blend of self-paced eLearning, instructor-led, and hands-on training provided at the customer site. This unique approach will help you stay more engaged and retain more learning, so you can see better results in your work right away. Upon completion of the course, you will be comfortable with setting up the instrument, optimizing compound and source parameters to create basic and advanced LC- MS/MS methods, acquiring data for a set of samples, performing quantitation, and carrying out instrument maintenance. Please refer to the workflow syllabus for focused topics and consult with your sales representative and Applications Manager to assess your specific training needs.

• Who should attend: Learners who need most effective training personalized to their instruments, workflows and experience levels.

• Pre-requisites: None. This course is intended for those with no experience in mass spectrometry.

• What's included:

o 0.5 Day (5 Hours) of instructor led training at the customer site by an experienced SCIEX Service Professional.

PUR 7776 (Revised 07/2022) Page 3 of 8 Incorporated by Reference: Rule 60A-1.045, F.A.C.

o 3 Days of instructor led training at the customer site by an experienced SCIEX Applications Support Scientist.

o 7-11 hours of guided eLearning covering topics such as theory, system operation, software, workflow and maintenance.

o Consumables required to complete the training.

o Hands-on training focused on 1 Primary Learner. 2 additional learners can join for observation and content.

o Online workflow certificate upon successful completion of final exam (Two levels available depending on the workflow: Basic Operator and Method Developer).

o P.A.C.E.® Continuing Education Credits: 18 credits upon completion of entire training session and a brief evaluation survey.

o Enrollment in related self-paced eLearning courses, lectures, reference material and lab exercises on the Learning Hub.

o Permanent access to all course materials for reference.

o Access to > 100 SCIEX Now Learning Hub Self-paced eLearning, lectures, demonstrations as well as SCIEX Now online Support tools available for up to 3 Learners.

o Complimentary follow-up virtual session with an Applications Support Scientist to ensure your success.

o Valid for 12 months from date of purchase. See latest course information in the SCIEX Now Learning Hub online catalog: https://sciex.com/support/training/course-catalog

• Related instruments: This course is currently offered for the following instruments – o MS Instruments: All SCIEX Triple Quad and QTRAP LC-MS systems (RUO versions) as well as ZenoTOF 7600, TripleTOF, X500R and X500B QTOF systems and the Echo MS System.

• Related workflows:

o Quantitation and screening in Clinical Research, Forensics, Environmental, Food and Beverage, Pharma/CRO on the SCIEX Triple Quad and QTRAP system.

o Quantitation and Screening on the X500R QTOF system.

o Biologics Characterization on the X500B QTOF system.

o Metabolite/impurity ID, Proteomics, Metabolomics, Pharma and Biopharma on the TripleTOF system.

o Biologics characterization, Quantitation and screening on the ZenoTOF 7600 system.

o Your workflow on the Echo MS system.

• Training location: Customer site

• Follow on courses:

o TRNLP006 Add-On 1 Day to any Success Program at Customer Site Part Number: 5077206 - MS Single Tuning Solution Kit ($0.00) MS Single Tuning Solution kit is a 3rd generation LC-MS system calibration kit which includes bottle A (calibration solution, 25mL) and bottle B (wash solvent, 25 mL). For use with SCIEX Triple Quad 7500 System- QTRAP ready.

Part Number: 4376878 - FG, Synergi 4U Fusion RP80A 50 X 2 ($0.00)

Part Number: 4376887 - Triazine Standard Mixture ($0.00)

PUR 7776 (Revised 07/2022) Page 4 of 8 Incorporated by Reference: Rule 60A-1.045, F.A.C.

System Suitability Test Mixture for Cliquid Food Testing package, Triazine Pesticides Standard Mix 100ug/mL each component in methanol, ampoule of 1mL.

Part Number: S202-DHR – Credit - $100,000 (TRIPLE6500+ S/N CF20981606) ($0.00) This trade in credit is contingent upon the purchase of the instruments and items on this quote and buyer satisfying all of the following requirements:

a. provide clear title to the instrument.

b. include the serial number of the trade in instrument on your P.O.

c. itemize the trade in dollar amount as a line item on your P.O.

d. return of a completed AB SCIEX Certificate of Decontamination (fax in advance of return, and original on outside of box/crate when shipped).

e. return the trade in instrument w/in 30 days of install of new instrument following the AB SCIEX returns procedure, including use of the Return Authorization number we will provide to you.

If Labs fail to meet any of these conditions, or do not receive a confirmation that AB SCIEX received the trade in instrument, you will be invoiced for and will be obligated to pay the full amount of the advance credit. If the trade in instrument is under an active AB SCIEX service plan, the remaining service plan balance/credit will be used to extend the coverage of your new instrument. Please do not turn off or decommission the trade in instrument until contacted by an AB SCIEX service engineer.

Intended Single Source:

AB Sciex, LLC

Estimated Dollar Amount:

$ 436,794.32 (Less Trade-In Credit: -$100,000.00 - For return of Triple Quad 6500+ S/N

CF20981606) $336,794.32

Justification for single source acquisition (what is necessary and unique about the product, service or source; steps taken to confirm unavailability of competition, as appropriate):

AB Sciex Pte. Ltd. is the original equipment manufacturer (and AB Sciex LLC the U.S. selling entity) of SCIEX Triple Quad 7500 System - QTRAP Ready (“Products”). AB Sciex LLC has the exclusive distribution and marketing rights in the U.S. for Products. In addition, AB Sciex LLC has granted (i) a healthcare distribution company a non-exclusive right in the U.S. to market and sell its Products to first time customer healthcare providers (NOT existing customers) whereby AB Sciex LLC accepts a lead from that distributer (disqualifying them from being a sourcing option in this instance), and (ii) in certain other circumstances through small and other than small businesses where our customers desire to purchase our products through such channels. With exception of those situations, AB Sciex LLC does not have any other authorized distributers of its Products or service for such Products. Ray Giska, Territory Manager for Mass Spec Sales - North Carolina | Ray.Giska@sciex.com | 919.623.6351 | Holly Springs NC, USA 27540 | 1201 Radio Road, Redwood City, CA 94065

Sole Source Justification and Specifications for the Acquisition of a Liquid Chromatograph/Tandem Mass Spectrometer (LC/MSMS) Instrument

PUR 7776 (Revised 07/2022) Page 5 of 8 Incorporated by Reference: Rule 60A-1.045, F.A.C.

The Florida Department of Environmental Protection, Chemistry Program, seeks to augment its analytical Ultra High-Performance Liquid Chromatography (UPLC) capabilities with the acquisition of an efficient liquid chromatograph/triple quadrupole mass spectrometer (LC/MSMS) for organic analysis. The LC/MSMS instrument is needed to handle an ongoing shortfall of UPLC trace-level analytical capacity. To adequately meet the needs of the chemistry laboratory, it is imperative to obtain instrumentation to provide enhanced analytical capabilities.

The LC/MSMS specifications, computer software, and data file format for the new instrument must be compatible with our existing equipment to maintain consistency, exchangeability, and integration. To adequately meet the needs of the chemistry laboratory, the LC/MSMS system must meet or exceed the following requirements and specifications. The instrument system must consist of a triple quadrupole mass spectrometer, instrument control software, and data reporting software.

A market review of LC/MSMS manufactures is also provided.

1. Mass Spectrometer Specifications:

1.1. The mass spectrometer must be a bench top triple quadrupole instrument capable of performing ultra-high sensitivity full-scan MS and MS/MS analysis. The sensitivity of the instrument in positive Multiple Reaction Monitoring (MRM) mode for 1 pg of Reserpine on column must give a S/N > 1,600,000:1. In negative MRM mode the instrument must give a S/N > 1600,000:1 for 1 pg of Chloramphenicol on column. The instrument design specifications are required to achieve the lab’s specified qualitative, quantitative, and detection limit objectives for a broad range of analytes in complex environmental samples.

1.2. The mass spectrometer must have a mass range (m/z) of at least 5 to 2000 Daltons (Da) with a mass stability of 0.10 Da in a 24-hour period. The mass range and stability requirements are needed to insure suitability for a wide range of potential environmental compounds of interest.

1.3. The mass spectrometer must be equipped with a curved acceleration collision cell enabling effective collection and transmission of ions, while minimizing space requirements and rejection of neutral species. This requirement ensures optimum performance in complex sample matrices and minimizes the overall size of the instrument.

1.4. The instrument system must be field-upgradeable to linear ion trap and quadrupole ion trap capabilities. This feature adds additional dimensions of selectivity necessary in some difficult analytical separations and helps prepare the instrument for emerging analytical applications during the operational life of the instrument.

1.5. The mass spectrometer must be capable of performing multiple reaction monitoring (MRM), neutral loss, and precursor ion scanning using scheduled MRM. The instrument must be capable of a minimum MRM dwell time of 1 msec, inter-MRM pause time < 3 msec, 12,000 Da/s scan speeds, and polarity switching of ≤ 5 msec. Operating the instrument under these fast collection conditions must have negligible impact to data quality and reproducibility, with under 10% variability as compared to slower data collection conditions. These scanning speed specifications are requirements for the laboratory’s LC/MSMS methods, saving instrument set-up and operational time.

1.6. The LC/MSMS must come equipped with a heated ion spray source capable of flow rates from 1.0 to 3000 µL/min without flow splitting or need to break vacuum to switch between flow configurations. The source must be capable of positive/negative mode switching; achieve temperatures up to 750°C in 1 °C increments; have an orthogonal spray configuration; probes and ion source which are automatically recognized/configured when installed; and compatible with

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100% organic-to-aqueous solvents. These specifications are designed to match the desired flow, minimize down-time, and achieve desired heating requirements of the LC and MS/MS instrument sub-components and methodology.

1.7. The LC/MSMS source must be equipped with a single electrode point source that creates an increased field strength for ESI operation. This specification improves the delivery of ions focused into the orifice as a result of the increased field strength from the lens, ultimately resulting in increased instrument sensitivity.

1.8. The LC/MSMS source must be capable of being equipped with a micro-column heater with a user settable temperature range from 5°C above ambient to 90°C, in 1°C increments. The micro-column heater is necessary when operating at micro-flow rates to minimize post-column band spread.

1.9. The mass spectrometer must have a detection system using a high voltage conversion dynode and pulse counting detector capable of 5 ms polarity switching and up to 6 orders of linear dynamic range. This specification allows for reduced method cycle times, allows for the collection of more MRM data points, and reduces interference from neutral molecular species when performing trace level analysis

1.10. The mass spectrometer must be compatible and ready to integrate (including all necessary hardware cables/connections and software) with the laboratory’s existing Shimadzu Nexera and AB Sciex EXION UPLC instruments, to which this mass spectrometer may be integrated. The main mass spectrometer software must be able to control the existing UPLC equipment, including the ability for online solid phase extraction and dual column analysis. The instrument must come with everything necessary to connect to our existing UPLC instruments. This specification is needed to provide trouble-free instrument connectivity, communication, and optimal chromatographic separation for the laboratory’s various analytical tests.

1.11. The mass spectrometer’s ion source interface must have an orifice rather than a heated capillary design. The direct orifice interface maximizes instrument performance and minimizes maintenance requirements.

2. Data System Specifications:

2.1. A complete, authorized software package for control of the liquid chromatograph and mass spectrometer is required. The software must include the capability to control auto-tuning, data acquisition, data analysis, method automation, macro-programming, data processing and reporting, automatic peak deconvolution capabilities, system management utilities, and be Windows® 10 compatible. Online solid phase extraction and dual column capabilities are also required functional features in the software package. Software is required to operate and report the LC/MSMS data.

2.2. The LC/MSMS software must be capable of qualitatively and quantitatively determining up to 300 compounds per injection in MRM mode with a minimum of 2 transitions per compound for identification and a minimum of 10 scans per compound for quantitation. To achieve this capability, the instrument software must perform measurements with dwell times as short as 1 millisecond. These features are needed to properly analyze and report many analytes in complex environmental samples.

2.3. The data system must have the ability to process previously acquired data from our existing AB SCIEX LC/MSMS instruments which utilize AnalystTM and MultiquantTM, and SCIEX OS software. This requirement regarding data processing compatibility will minimize staff training, PUR 7776 (Revised 07/2022) Page 7 of 8 Incorporated by Reference: Rule 60A-1.045, F.A.C.

ensure ability to evaluate historical data, streamline on-going data processing, and simplify storage/recovery of archived data.

2.4. The data system must be capable of scheduled MRM whereby the instrument scans for ions of interest only when these ions are expected to enter the instrument. The data system must also perform automated peak integration, automatic outlier flagging/removal, and standard addition natively in the software. These instrument optimization features may provide increased instrument sensitivity, optimal system performance, reduced human error, and time savings for staff.

2.5. The software must have an optional e-signature and audit trail tools used to achieve 21 CFR Part 11 compliance.

3. Service, Installation, and Training:

3.1. The Service Provider must include delivery and installation of all equipment, including checkout and verification of performance. The Service Provider must offer on-site services including all labor, parts, and materials required to maintain the hardware in good operating condition. This provides immediate verification that the instrument is working properly before the laboratory officially accepts the purchased equipment.

3.2. The Service Provider must be the original equipment manufacturer (OEM) or a certified manufacturer distributor. The equipment must not be modified against the original manufacturer’s recommendation. This ensures that the instrument does not void the OEM warranty and that service and parts will be available during the life of the instrument.

3.3. The LC/MSMS and Chromatography Data Acquisition System must include maintenance warranty and technical support of at least one (1) year including all parts, software upgrades, labor and travel, and have available optional enhanced/extended warranty packages. Warranty service is needed to mitigate potential instrument failure and reduce instrument down-time.

3.4. The vendor must provide ongoing service agreements using OEM-trained service engineers for the entire LC/MSMS system, including the UPLC, mass spectrometer, computer, and data collection system.

3.5. The LC/MSMS vendor must guarantee in writing a maximum response time of three hours for service calls via telephone whenever the system is inoperable, and a maximum response time of two workdays for on-site service whenever telephone consultation cannot resolve the problem.

Both response times are defined as the length of time from first contact by DEP regarding an inoperable system to either the receipt of a telephone response from a service engineer or the arrival of a service engineer. This requirement will help minimize down-time and disruption to laboratory services.

3.6. The vendor must guarantee in writing that technical support will be provided for the lifetime of the System and that service contracts will be available to the Department for the instrument at least seven (7) years after delivery. This helps ensure the laboratory can receive quality service over the life of the instrument.

3.7. Service must include an assigned Account Customer Engineer and an assigned backup Customer Engineer to insure uninterrupted service to our laboratory.

3.8. The Service Provider must include instrument familiarization/training at the time of instrument installation or at a mutually agreed time following instrument installation.

Companies and Instrument Systems Considered in Our Market Review:

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The following companies and instrument systems were considered in this review and were evaluated by the previously listed specifications. There may be additional specifications, besides those listed, that are not fully met by the excluded vendors.

Company: AB SCIEX LLC Instrument: 7500 Triple Quad Mass Spectrometer Comments: Selected Instrument.

Company: Agilent Technologies Instrument: 6495C Triple Quad Mass Spectrometer Comments: Does not meet specification 1.1, 1.4, 1.5, 1.7, 1.10, and 1.11.

Company: Bruker Instrument: EVOQ Elite Triple Quad Mass Spectrometer Comments: Does not meet specification 1.1, 1.2, 1.4, 1.5, 1.7, 1.8, and 1.11.

Company: Perkin Elmer Instrument: QSight 400 Triple Quad Mass Spectrometer Comments: Does not meet specification 1.3, 1.4, 1.7, 1.10, and 1.11.

Company: Thermo Scientific Instrument: TSQ Altis Triple Quad Mass Spectrometer Comments: Does not meet specification 1.4, 1.5, 1.6, 1.7, 1.10, and 1.11.

Company: Shimadzu Instrument: LCMS-8060NX Triple Quad Mass Spectrometer Comments: Does not meet specification 1.1, 1.3, 1.4, 1.7, and 1.11.

Company: Waters Instrument: Xevo Absolute and TQ-series Triple Quad Mass Spectrometers Comments: Does not meet specifications 1.3, 1.4, 1.5, 1.6, 1.7, 1.9, and 1.10.

After a careful market review, we have concluded that AB SCIEX is the only vendor that can provide the LC/MSMS instrumentation compatible with our existing laboratory operations and analytical needs. Therefore, we request permission to purchase the 7500 instrument from AB SCIEX on a single source basis. A price quotation is attached.

Approved By (names & titles, as appropriate, e.g., requestor, requestor management, information systems, budget, purchasing):

Colin Wright, Program Administrator Adam Blalock, Deputy Secretary of Ecosystem Restoration Belinda Croft, Purchasing Specialist Supervisor

Prospective vendors are requested to provide information regarding their ability to supply the commodities or contractual services described. If it is determined in writing by the agency, after reviewing any information received from prospective vendors, that the commodities or contractual services are available only from a single source, the agency shall provide notice of its intended decision to enter a single-source purchase contract in the manner specified in Rule 60A-1.045, Florida Administrative Code, and section 120.57(3), F.S.