PUR_7776.pdf

PUR 7776 (Revised 07/2022) Page 1 of 2

Incorporated by Reference: Rule 60A-1.045, F.A.C.

DESCRIPTION OF INTENDED

SINGLE SOURCE PURCHASE

(PUR 7776)

AGENCY: Florida Fish and Wildlife Conservation Commission (FWC)

TITLE: KASP Genotyping Assay Development for Corals

Short description of the commodity or service desired: Species-specific genotyping assay used to genotype coral samples from the Coral Rescue Project.

CONTACT

Name: Matthew Carroll

Address: 1875 Orange Ave E, Tallahassee, FL 32311

Telephone: 850-617-9692

Email: Matthew.Carroll@myfwc.com

Internal tracking number, if any: FWC SS 23/24-07

Date posted: 4/5/2024 Last day for receipt of information: 4/26/2024

This description of commodities or contractual services intended for purchase from a single source is posted in accordance with Sections 120.57(3) and 287.057(3), Florida Statutes (F.S.), and will remain posted for a period of at least 15 business days.

Commodity or Contractual Service Required (commodity or United National Standard Products and Services Code (UNSPSC), manufacturer, model, and description, as appropriate):

85131709: Genetics research services

Quantity or Term (as appropriate):

Three (3) Calendar Years from issuance of 1st purchase order.

Requestor (division, bureau, office, individual, as appropriate):

Alicia Vollmer

Performance and/or Design Requirements (e.g. intended use, function or application, compatibility, requirements; reference to policy, rule, statute or other act of the Legislature, etc., as appropriate):

The intended use of the Kompetitive Allele Specific PCR (KASP™) genotyping assays is to genotype the corals collected as part of the Coral Rescue Project. Over 3,000 corals were collected from the wild as part of the Coral Rescue Project, a joint effort between FWC and

NOAA, and placed in land-based facilities to preserve genetic diversity and propagate them for restoration of Florida's Coral Reef in response to stony coral tissue loss disease. All of the corals in holding are the property of FWC and are on loan to authorized aquariums around the US. All of the corals in holding need to be genotyped to identify unqiue individuals for breeding and propagation. These KASP genotyping assays use 192 species-specific single nucleotide polymorphism (SNP) markers discovered during the genotyping-by-sequencing process to

PUR 7776 (Revised 07/2022) Page 2 of 2

Incorporated by Reference: Rule 60A-1.045, F.A.C.

develop a genotyping assay in which coral samples are analyzed. Once the assay is created for each coral species, it can be repeatedly used to genotype additional corals that are collected, identify the genetic diversity of baby corals that are propogated in hodling facilities or examine the relatedness of outplanted populations at restoration sites. The genetic infromation from the

KASP assay (and the genotyping data) is then entered into the FWC Genetic Registry, which houses all of the raw genetic data and allows staff to create genetically informed breeding and restortaion plans for the corals.

Intended Single Source:

LGC Biosearch Technologies

Estimated Dollar Amount:

$275,000.00 (~$16,000 per species) x 16 species w/ additional $3,000.00 buffer.

Justification for single source acquisition (what is necessary and unique about the product, service or source; steps taken to confirm unavailability of competition, as appropriate):

Kompetitive Allele Specific PCR (KASP™) assays enable accurate, bi-allelic discrimination of single nucleotide polymorphism (SNPs) markers. This is a proven method for genotyping corals within the Coral Rescue Project as we have created KASP assays for 5 coral species already. We have attempted other techniques for creating genotyping assays that can be used for corals, but they have failed and provide unusable results. The KASP assay method has an accuracy rate of

>99.8% based on an independent assessment and is an industry leading SNP conversion rate. The

KASP-TF Master Mixes, KASP Assays (KASP By Design, and KASP On Demand) and KASP

All-Inclusive genotyping services are sole source products and services manufactured and operated by the LGC Biosearch Technologies, and for which the company own the patent rights.

Approved By (names & titles, as appropriate, e.g., requestor, requestor management, information systems, budget, purchasing):

Alicia Vollmer, Requestor

James Kozlowski, Budget

Caroline Gorga, Section Leader

Leanne Flewelling, Deputy Director

Rachel Korade - General Services Purchasing Manager

Renee Gregory - Purchasing Specialist Supervisor

Prospective vendors are requested to provide information regarding their ability to supply the commodities or contractual services described. If it is determined in writing by the agency, after reviewing any information received from prospective vendors, that the commodities or contractual services are available only from a single source, the agency shall provide notice of its intended decision to enter a single-source purchase contract in the manner specified in Rule 60A-

1.045, Florida Administrative Code, and section 120.57(3), F.S.