PROJECT DETAILS - CYAGEN.pdf

Service /Projects to be Performed /Fulfilled by Cyagen

1. Mouse Themis3 Conditional Knockout Project (C57BL/6N)

[SPRCH-01006] Purchase BAC from CHORI - BACs purchased from CHORI - Turnaround time: 4-5 weeks - Shipped and handled by CHORI - QTY (1) EA

[STKMU-02001] TurboKnockout® Conditional Knockout - Vector Construction (C57BL/6N) - Turnaround time: 8-14 weeks. - Deliverable: TurboKnockout® Gene Targeting Report. - Design and construction of TurboKnockout® conditional knockout vector after client approval of Cyagen-proposed TurboKnockout® gene targeting strategy. - QTY (1) EA

[STKMU-02002] TurboKnockout® Conditional Knockout - Targeting in ES Cells (C57BL/6N) - Turnaround time: 8-14 weeks. - Deliverable: Correctly Targeted and Verified ES Cell Genotyping Report. - TurboKnockout® Vector will be purified, linearized, and electroporated into ES cells. ES cells are subjected to drug selection, screened by long range PCR, and verified for correct gene targeting by Southern blot. - QTY (1) EA

[STKMU-02005] TurboKnockout® Conditional Knockout - Karyotype ES Cells, clone (C57BL/6N) - Turnaround time: 1-2 weeks. - Chromosome counting is performed to ensure ES cells have the correct number of chromosomes. - QTY (2) EA

[STKMU-02003] TurboKnockout® Conditional Knockout - Mouse Generation (C57BL/6N) - Turnaround time: 8-12 weeks. - Deliverable: A minimum of 3 TurboKnockout® heterozygous mutant mice - QTY (1)

EA

2. Mouse Trat1 Conditional Knockout Project (C57BL/6N)

[SPRCH-01006] Purchase BAC from CHORI - BACs purchased from CHORI - Turnaround time: 4-5 weeks - Shipped and handled by CHORI – QTY (1) EA

[STKMU-02001] TurboKnockout® Conditional Knockout - Vector Construction (C57BL/6N) - Turnaround time: 8-14 weeks. - Deliverable: TurboKnockout® Gene Targeting Report. - Design and construction of TurboKnockout® conditional knockout vector after client approval of Cyagen-proposed TurboKnockout® gene targeting strategy. - QTY (1) EA

[STKMU-02002] TurboKnockout® Conditional Knockout - Targeting in ES Cells (C57BL/6N) - Turnaround time: 8-14 weeks. - Deliverable: Correctly Targeted and Verified ES Cell Genotyping Report. - TurboKnockout® Vector will be purified, linearized, and electroporated into ES cells. ES cells are subjected to drug selection, screened by long range PCR, and verified for correct gene targeting by Southern blot. - QTY (1) EA

[STKMU-02005] TurboKnockout® Conditional Knockout - Karyotype ES Cells, clone (C57BL/6N) - Turnaround time: 1-2 weeks. - Chromosome counting is performed to ensure ES cells have the correct number of chromosomes. - QTY (2) EA

[STKMU-02003] TurboKnockout® Conditional Knockout - Mouse Generation (C57BL/6N) - Turnaround time: 8-12 weeks. - Deliverable: A minimum of 3 TurboKnockout® heterozygous mutant mice - QTY (1)

EA

3. Mouse Fcer1g Conditional Knockin Project (C57BL/6N)

[SPRCH-01006] Purchase BAC from CHORI - BACs purchased from CHORI - Turnaround time: 4-5 weeks - Shipped and handled by CHORI - QTY (1) EA

[SPRCH-01005] cDNA Synthesis - CDS of Exon 4~5-Flag-3’UTR (401 bp) - Mutant CDS of Exon 4~5-Myc- 3'UTR (407 bp) - Turnaround time: 3-4 weeks - QTY (1) EA

[STKMU-03001] TurboKnockout® Global Knockin - Vector Construction - Turnaround time: 8-14 weeks. - Deliverable: TurboKnockout® Gene Targeting Vector Report. - Design and construction of TurboKnockout® global knockin vector after client approval of Cyagen-proposed TurboKnockout® gene targeting strategy. - QTY (1) EA

[STKMU-03002] TurboKnockout® Global Knockin - Targeting in ES Cells - Turnaround time: 8-14 weeks. - Deliverable: Correctly Targeted and Verified ES Cell Genotyping Report. - TurboKnockout® Vector will be purified, linearized, and electroporated into ES cells. ES cells are subjected to drug selection, screened by long range PCR, and verified for correct gene targeting by Southern blot. - QTY (1) EA

[STKMU-03005] TurboKnockout® Global Knockin - Karyotype ES Cells, clone - Turnaround time: 1-2 weeks. - Chromosome counting is performed to ensure ES cells have the correct number of chromosomes. - QTY (2) EA

[STKMU-03003] TurboKnockout® Global Knockin - Mouse Generation - Turnaround time: 8-12 weeks. - Deliverable: A minimum of 3 TurboKnockout® heterozygous mutant mice - QTY (1) EA

4. Mouse Cd3e Conditional Knockin Project (C57BL/6N)

[SPRCH-01006] Purchase BAC from CHORI - BACs purchased from CHORI - Turnaround time: 4-5 weeks - Shipped and handled by CHORI - QTY (1) EA

[SPRCH-01005] cDNA Synthesis - CDS of exon 7-8-Flag-3'UTR - Mutant CDS of Exon 7~8-Myc-3'UTR - Turnaround time: 3-4 weeks - QTY (1) EA

[STKMU-03001] TurboKnockout® Global Knockin - Vector Construction - Turnaround time: 8-14 weeks. - Deliverable: TurboKnockout® Gene Targeting Vector Report. - Design and construction of TurboKnockout® global knockin vector after client approval of Cyagen-proposed TurboKnockout® gene targeting strategy. - QTY (1) EA

[STKMU-03002] TurboKnockout® Global Knockin - Targeting in ES Cells - Turnaround time: 8-14 weeks. - Deliverable: Correctly Targeted and Verified ES Cell Genotyping Report. - TurboKnockout® Vector will be purified, linearized, and electroporated into ES cells. ES cells are subjected to drug selection, screened by long range PCR, and verified for correct gene targeting by Southern blot. - QTY (1) EA

[STKMU-03005] TurboKnockout® Global Knockin - Karyotype ES Cells, clone - Turnaround time: 1-2 weeks. - Chromosome counting is performed to ensure ES cells have the correct number of chromosomes. - QTY (2) EA

[STKMU-03003] TurboKnockout® Global Knockin - Mouse Generation - Turnaround time: 8-12 weeks. - Deliverable: A minimum of 3 TurboKnockout® heterozygous mutant mice - QTY (1) EA

Customer has full ownership rights of the above-mentioned mouse model including but not limited to, performing research, obtaining patents and other intellectual property rights, commercializing, breeding and selling/sharing to other third parties.