PF250238TC - DFW-245847 - Axioscope 7 RFQ.pdf

WASHINGTON DEPARTMENT OF FISH AND WILDLIFE REQUEST FOR QUOTATION

Return Quotation To:

Washington Department of

Fish and Wildlife Contracts and Purchasing

Quotation Number:

PF250238TC

Mailing Address:

WDFW Purchasing Attn: Tina Carollo PO Box 43135 Olympia WA 98504-

Physical Address (for couriers):

Natural Resources Bldg. (5th Floor), 1111 Washington St. SE Olympia WA 98501

Ship To Address: (FOB Destination, Freight Prepaid, Allowed) Freight/shipping costs included in the bid price

Washington Department of Fish and Wildlife 1111 Washington Street SE 2nd floor IT Olympia WA 98501 United States

Contact Name: Tina Carollo E-mail: tina.carollo@dfw.wa.gov Fax No. due to COVID-19, faxes not allowed Quotation Required By (Date and Time):

April 25, 2025 12:00 Noon

Required Delivery Date:

Product must be received by COB 6/30/25 or will be refused at no cost to WDFW.

VENDOR QUOTATION

Vendor Name: Name of Person providing Quotation:

ITEM NO. SPECIFICATIONS QTY UNIT UNIT PRICE TOTAL PRICE

Minimum Specifications provided – All _13___pages to be completed and returned with quote In addition to the initial purchase quantity identified in this bid document there is no guarantee to the contractor of any additional orders under the award. However, the order will allow the placement of future purchase orders on an as needed basis (also referred to indefinite delivery – indefinite quantity orders) under the same terms and conditions as those established for the initial purchase quantity within one year of award of the bid

Destination WA State Sales Tax Applies, added to billing invoice (not w/bid) Subtotal:

Any and all questions must be directed to Tina Carollo @ tina.carollo@dfw.wa.gov. Any vendors that make contact with anyone outside of the WDFW contracts and purchasing office could result in their bid being disqualified.

VENDOR INFORMATION

Vendor Name: UBI No. Statewide Vendor Number:

Vendor Street Address: Vendor Mailing Address (if different):

Federal TIN/SSN:

E-mail: Telephone No. Fax No.

Vendor’s Proposed Delivery Date: Prompt Payment Discount:

_____% discount for payment within _____ days OMWBE Minority or Women-Owned Business Certification Number (if applicable):

Department of Veteran Affairs Veteran-Owned Business Certification Number (if applicable)

Type/Print Name of Person Signing Quotation:

Authorized Signature and Date Signed:

INSTRUCTIONS FOR VENDORS

1. This Request for Quotation (RFQ) is not an order. If your quotation is accepted WDFW will issue a Field Order or other order document that will indicate what items have been accepted. WDFW may accept or reject quotations by each item separately or as a whole.

2. Please provide a price quote via fax, email or regular mail for the item(s) specified in this RFQ.

3. Please complete the VENDOR QUOTATION, VENDOR INFORMATION and VENDOR CERTIFICATION EXECUTIVE ORDER 18-03 pages of this form to respond to this request. Failure to complete and use this form may result in rejection of a vendor’s quote.

4. Responses for the items indicated must be returned by no later than the date and time indicated.

5. Unless otherwise indicated, all prices offered must be FOB Destination, with all transportation and handling charges paid by the Vendor.

6. All quotations provided by the Vendor are good for a minimum of 60 calendar days from the Quotation Required By date/time on Page 1 of this document.

7. Vendors must be registered in the Statewide Vendor Payment System prior to submitting any requests for payment.

The Washington State Department of Enterprise Services (DES) maintains the Statewide Vendor Payment System; to obtain registration materials contact the Statewide Payee Desk at (360) 407-8180; or go to Statewide mailto:tina.carollo@dfw.wa.gov https://ofm.wa.gov/it-systems/accounting-systems/statewide-vendorpayee-services

Vendor/Payee Services | Office of Financial Management (wa.gov)

8. Washington Department of Fish and Wildlife Additional Terms and Conditions for Requests for Quotations and Field Orders govern this RFQ. These terms and conditions can be found at https://wdfw.wa.gov/about/administration/contracts Deviations from, or additions to, these terms are attached.

By signing this RFQ the Vendor certifies that they have read, understand, and agree to these terms and conditions.

VENDOR CERTIFICATION EXECUTIVE ORDER 18-03 – WORKERS’ RIGHTS WASHINGTON STATE GOODS & SERVICES

CONTRACTS

Pursuant to RCW 39.26.160(3) (best value criteria) and consistent with Executive Order 1803 – Supporting Workers’ Rights to Effectively Address Workplace Violations (dated June 12, 2018), the Washington Department of Fish and Wildlife will evaluate bids for best value and provide a bid preference in the amount of 3% to any bidder who certifies, pursuant to the certification below that their firm does NOT require its employees, as a condition of employment, to sign or agree to mandatory individual arbitration clauses or class or collective action waiver.

I hereby certify, on behalf of the firm identified below, as follows (check one):

� NO MANDATORY INDIVIDUAL ARBITRATION CLAUSES AND CLASS OR COLLECTIVE ACTION WAIVERS FOR EMPLOYEES. This firm does NOT require its employees, as a condition of employment, to sign or agree to mandatory individual arbitration clauses or class or collective action waivers.

OR

� MANDATORY INDIVIDUAL ARBITRATION CLAUSES AND CLASS OR COLLECTIVE ACTION WAIVERS FOR EMPLOYEES.

This firm requires its employees, as a condition of employment, to sign or agree to mandatory individual arbitration clauses or class or collective action waivers.

I HEREBY CERTIFY, UNDER PENALTY OF PERJURY UNDER THE LAWS OF THE STATE OF WASHINGTON, THAT THE CERTIFICATIONS HEREIN ARE TRUE AND CORRECT AND THAT I AM AUTHORIZED TO MAKE THESE CERTIFICATIONS ON

BEHALF OF THE FIRM LISTED HEREIN.

FIRM NAME: _____________________________________________________

Name of Vendor – Print full legal entity name of firm

By: ______________________________ Signature of authorized person https://ofm.wa.gov/it-systems/accounting-systems/statewide-vendorpayee-services https://wdfw.wa.gov/about/administration/contracts

Title: ______________________________ Title of person signing certificate

Date: ________________________________

Print Name of person making certifications for firm

Place: ________________________________ City and state

SPECIFICATIONS FOR:

Zeiss Axioscope 7 for histology and bacteria with motorized stage, 3-channel fluorescence, brightfield, Zen software, and motorized acquisition toolkit.

PURPOSE:

ADVANCED IMAGING

FUNCTION:

We are currently unable to do our own histological microscopy, requiring us to outsource the work. This advanced imagining microscope will satisfy the shared histological needs for both TBios and Fish Health. In addition, the microscope will also enable fluorescence imaging to detect Renibacterium salmoninarum, the causative agent of bacterial kidney disease. Although the primary purposes of the microscope are for histology and fluorescence imaging, the scope will also be shared resource with the Otolith and Aging labs.

Note: Polychlorinated biphenyls, commonly known as PCBs, have adverse effects on human health and the environment.

Accordingly, the State of Washington through its procurement of goods is trying to minimize the purchase of products with PCBS and to incentive its vendors to sell products and products in packaging without them. WDFW respectfully requests any packaging be without color or dyes.

For Contracts and Awards with an Estimate Value under $150,000 The Purchaser is issuing this Solicitation pursuant to RCW 39.26. Pursuant to this Solicitation, the Purchaser intends to conduct a competitive procurement to award an Agency Contract estimated to be valued at less than $150,000. Because it is estimated to be less than $150,000, the Purchaser intends to award the contract to the lowest responsive and responsible bid from a Small Business or Veteran-Owned Business, unless none of these businesses are responsible and responsive to this solicitation

Item Description Check If As Specified

Describe Fully If Not As Specified

Microscope stand Axioscope 7, TL/FL MOT, 6x H DIC Encoded

Microscope stand Axioscope 7, TL/FL MOT, 6x H DIC encoded - lower stand part with dovetail illumination interface

- transmitted-light illumination with white LED 10W, optional for HAL 100W - dovetail mount for attachable stage carriers -motorized parfocality function - 24 mm motorized focus lift (min. step width 78 nm), adjustable focus stop - auto mechanical safety shutter - 6-position filter wheel for dia. 36 mm filters -mount for 2-position filter slider 14x40, d=36 mm - CAN port for Colibri/HXP - integrated 24V DC 100W power unit, stabilized 100...240V AC/ 50...60Hz - country-specific power cable - ECO mode and light management control button - snap button supports on ZEISS Axiocam

Binocular Ergophototube 20°/23 (100:0/0:100), reversed image variable, continuous vertical adjustment 44 mm, camera port with interface 60N

Eyepiece PL 10x/23 Br. foc.

Solid-State Light source Colibri 3, Type

GB-UV Solid-State Light source Colibri 3, Type GB-UV 3-channel fluorescence light source with integrated control unit in Axioscope stand for continuous brightness adjustment, quickly switchable and adjustable by stand alone, OnScreenDisplay (OSD) in Axiocam 202, software Labscope App. Equipped with 3 solid state LED lamps. - Green (565nm) for excitation of Cy3, TRITC, DsRed and similar dyes - Blue (470nm) for excitation of eGFP, Fluo4, FITC and similar dyes - UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258

Mechanical stage 75x50 mot standard;

CAN (D)

Scope, Axio Examiner, Axio Imager and SteREO Solid-State Light source Colibri 3, Type GB-UV 3-channel fluorescence light source with integrated control unit in Axioscope stand for continuous brightness adjustment, quickly switchable and adjustable by stand alone, OnScreenDisplay (OSD) in Axiocam 202, software Labscope App. Equipped with 3 solid state LED lamps. - Green (565nm) for excitation of Cy3, TRITC, DsRed and similar dyes - Blue (470nm) for excitation of eGFP, Fluo4, FITC and similar dyes - UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258

Joystick XY; CAN (D) Converter CAN

- USB Rev.2 (D) Interface for components with CAN to USB PC Sockets: 1x CAN, 1x USB, 24 V Included accessories:

power supply 100

- 240 V with country specific plug, USB cable

Specimen holder, special, for 2 slides 76x26 mm 2 speed ranges selectable

- direct cable connection to CAN stage or to stage controller

Stage carrier D/A; attachable and vertically adjustable for Axioscope 5 to accommodate screw-on stages

Reflector turret 4x encoded, changeable, for P&C module

Filter Set 90 HE LED with Reflector Module FL EC P&C (E) for Colibri

Filter Set 90 HE LED

Filter Set 90 HE LED with Reflector Module FL EC P&C (E)

Filter Set 90 HE LED shift free for use with Colibri 5/7 with excitation wavelengths 385, 475, 555 and 630 nm. Consisting of filters QBS 405 + 493 + 575 + 653 and QBP 425/30 + 514/30 + 592/30 + 709/100 inserted in reflector module, reflection avoided through tilted mount for emission filters

Analyzer module DIC ACR P&C for transmitted light optimized for DIC in transmitted light, not suitable for polarization

Condenser carrier with vertical adjustment on both sides for use with attachable Axio Scope stage carriers, adjustable height stop

Condenser 0.9/1.25 H AxioLab / AxioScope Condenser 0.9/1.25 H For objectives 5x-100x, with low-power system from 2.5x, WD=0.8mm

Objective EC Plan-Neofluar 10x/0.3 M27 (FWD=5.2mm

Objective EC Plan-Neofluar 20x/0.50 M27 (FWD=2.0mm

Objective EC Plan-Neofluar 40x/0.75 M27 (FWD=0.71mm), incl. Cover glasses, high performance, CG=0.17mm, box with 100 pc.

Objective EC Plan-Neofluar 100x/1.30 Oil M27 (FWD=0.20mm), incl. Immersol 518 F, oiler 20ml and Cover glasses, high performance, CG=0.17mm, box with 100 pc.

DIC slider EC PN 10x/0.30 I, 20x/0.50 II, PA 20x/0.80 II

DIC slider EC PN 40x/0.75 II, EC PN 100x/1.30 III

Condenser module DIC I/0.9 with polarizer - AxioScope

Condenser module DIC II/0.9 with polarizer - Axio Scope

Condenser module DIC III/0.9 with polarizer - Axio Scope

Microscopy Camera Axiocam 212 color

Most versatile color camera for education, documentation and routine applications with standalone capability, USB 3.0 and Ethernet connectivity with 64bit driver software for: ZEN (blue and core), Labscope and TWAIN plugin. Camera package contains the following accessories: - USB 3.0 flash drive, Type- C and Type-A - USB

3.0 cable Type-C to Type-A - Power & signal cable camera to microscope stand compatible with Axiolab 5 and Axioscope 5/7/Vario - Power adapter with country-specific plug Sensor:

Sony CMOS image sensor Color, Rolling Shutter Number of Pixel (max): 4032

(H) x 3044 (V) = 12.3 MP, Full Sensor Selectable Resolution: 4032 (H) x 3044

(V) = 12.3 MP, Full Sensor 3840 (H) x 2160 (V) = 8.1 MP, Ultra HD (4K) 1920 (H) x 1080 (V) = 2.1 MP, Full HD (1080p) Pixel size: 1.85 μm x

1.85 μm (resolution 4032 x 3044 and 3840 x 2160) 3.70 μm x

3.70 μm (binned, resolution 1920 x 1080) Effective Sensor Area: 4032 x 3044: 7.4 mm x 5.7 mm, equivalent to 1/1.7" (9.3 mm diagonal) 3840 x 2160: 7.2 mm x

4.0 mm, equivalent to 1/2.1" (8.2 mm diagonal) 1920 x 1080:

7.2 mm x 4.0 mm, equivalent to 1/2.1" (8.2 mm diagonal)

Microscopy Camera Axiocam 305 mono R2 (D) Microscopy camera incl. driver software 64bit, USB

3.0 PCIe x1 interface, USB 3.0 cable 3 m and BK7 protection glass (coated) Number of Pixels: 2464 (H) x 2056 (V) = 5.07 Mega pixel color Sensor type: SONY IMX264 Exmor Pregius Global Shutter CMOS Pixel size: 3.45 μm x 3.45 μm Chip size:

8.5 mm x 7.1 mm, equivalent to diagonal 11.1 mm (2/3" form factor) Spectral range: app. 350 nm to 1000 nm Full Well Capacity: Approx.

10,500 e Selectable Resolution: H x V Binning Factor 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 816 x 684 3x3 608 x 514 4x4 480 x 410 5x5 H xV Subsampling Factor 2464 x 2056 1x1 Basic Resolution 1232 x 1028 2x2 Live frame rates (depending on hardware and software configuration): H x V Frame Rate@1ms 2464 x 2056 36 fps 1232 x 1028 88 fps (2x2 Subsampling) Frame rates for time series recording (depending on computer hardware and software configuration) H x V (ROI) Frame Rate 2464 x 2056 36 fps 2048 x 2048 36 fps 1920 x 1080 67 fps 1024 x 1024 70 fps 512 x 512 136 fps 256 x 256 255 fps 128 x 128 456 fps

Camera Adapter 60N-C 2/3'' 0.63x

Camera Adapter 60N-C 1'' 1.0x

Double adapter 60N - 2x 60N with slider mount - slider mount for slider with 100% mirror or slider with beam splitter mount

- 2 clickstop positions

- mount for filters d=25 mm on both exits alternatively required:

- slider with 100% mirror or

- slider with beam splitter mount and 50:50 beam splitter or other beam splitters with dimensions 26x36 mm

Slider with 100% mirror for double adapter - 2 clickstop positions for exit straight / lateral

ZEN 3.11 11 x64 User interface configurable, control of the ZEISS microscope systems and components, extensive acquisition and analysis, CZI image format The following functions are included: - Basic 3D viewing - Colocalization - Connect basic - Direct processing - Manual EDF - Measurement - Panorama - Spectral unmixing - ZEN Data Storage Client - Acquisition Base (time series and multichannel)

ZEN Toolkit Motorized Acquisition Acquire images and control motorized components in multi-dimensional experiments. This package allows to acquire tiles, multi-position and z-stack images using motorized components.

These can be combined with the included autofocus and direct processing. z-stack and enhanced depth of focus functionality:

- definition of the Zstack in "Start/Stop" and "Center" mode -size of the configurable Z-stack and number of possible Z-planes only limited by the travel range of the system and the minimum increment - "Optimal" button to automatically set the correct increment to satisfy the Nyquist criterion - integrated Z-drive backlash compensation for maximum precision and reproducibility -

SPECIFICATIONS- DELIVERY & PAYMENT

Item Description Check If As Specified

Describe Fully If Not As Specified

Product must be received by COB 6/30/25 or will be refused at no cost to WDFW.

Deliver to:

Washington Department of Fish and Wildlife 1111 Washington Street SE 2nd Floor IT Olympia, WA 98501 United States

Delivery Hours: Mon – Fri. 9:00 am – 4:00 pm Payment terms: WDFW will process payment

(net 30 days) to the awarded vendor at the time of conclusion of all goods and services having been received, location contact has satisfactorily accepted the delivery and vendor provides proper invoice.

Submitted bid needs to have literature (brochure) with specifications and picture of the item’s make and model available and ready

PRICE SHEETS

Req.

Item

Comm.

Code

Description

PF250 TC

Qty Unit Unit Price Total Price

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________

EA $ $

Brand/Model: __________ For Product Bid State:

Mfg.:_______________

For Product Bid State:

Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

For Product Bid State: EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________ Brand/Model: __________

EA $ $

For Product Bid State:

Mfg.:_______________