A02- MEC_Final.pdf

Integrated Automated Rodent Ac vity & Behavioral Monitoring System

The U.S. Army Medical Research Ins tute of Chemical Defense (USAMRICD) requires a fully integrated so ware system allowing automated behavioral ac vity monitoring, gait tracking, and locomo on examina on compa ble with various chambers/apparatus for evalua ng rat movement with both live and recorded video capability.

All equipment needs to run the same so ware for data cohesion, simplified analysis, and to prevent delays or complica ons in learning new collec on or data transfer systems to meet research melines.

x1 - EthoVision XT Behavioral analysis so ware for small animals (specifically rat) and associated license keys o Use with mul ple types of chambers, field, or maze environments to include Light Dark

Box systems, Shu le box, and Operant chambers o Precise monitoring to determine animal ac vity, loca on, posture, and body elonga on;

3-point tracking to obtain precision collec on while minimizing compu ng burden o Data collec on from mul ple areas, tracking of up to 100 individuals, social interac on evalua on, and rat behavior recogni on in low contrast environments o Collects mul ple parameters to include velocity, distance, me spent in loca on, immobiliza on, and ac vity detec on; similar data language as The Observer XT o Data categoriza on of video monitoring that outputs behavioral phenotypes or behavioral categories, along with the rela ve dura ons of each phenotype, their frequency, or some other relevant measure for comparison within- and between-subjects o Simultaneous live feed and recording for up to 24 hours, as well as expor ng of video into a non-proprietary format and poten al for picture in picture features for viewing;

must be compa ble with Windows 10 or 11 opera ng system and Trellix (former

McAfee) an -virus pla orm o So ware use should include minimal to no training and exper se to use, no level of coding experience required, near plug and play feature including interface templates to run data collec on with minor manipula on of parameters o Data analysis tools such as trial by trial, group interac on, me-based analysis, and area related comparisons included as well as graphing features of these data o Compa ble with DSI physiological recording system Ponemah for use with instrumented animals x2 EthoVision XT Behavioral analysis so ware for small animals (specifically rat) and associated license keys with above features as well as compa ble func on with PhenoTyper 4500 equipment of lick-o-meter, running wheel, buzzers, etc. within chamber.

x1 - Compa ble GigE camera set (=2) system to match interface/use with exis ng GigE camera set inhouse o Able to operate in darkened environments (e.g., IR sensi ve with filter and lens); gigabit ethernet interface or easy/low power connec on to systems running so ware and/or behavioral equipment o Mountable with moun ng equipment included no drilling required (pole extension ~6-

12.5 feet); sani zable surface o Needs to be compa ble to Light Dark Box and PhenoTyper 4500 chamber systems

X3 - Desktop worksta on included with EthoVision XT, and any associated camera so ware already updated, installed, tested and validated by manufacturer prior to delivery - to run Light

Dark Box chamber and 8 Unit Ac vity PhenoTyper o Fast SSD, sufficient HDD/RAM, and powerful video card to func on with data collec on, analysis, and storage o Needs to be compa ble with GigE camera recording, streaming, and file storage to include 24hr recording o Mul ple systems needed so all chambers can be run simultaneously o Compa ble with DHA image requirements and runs with Windows 10 or 11 opera ng systems o Include Manufacturer’s warranty o Must be TAA-compliant x1 - Light Dark Box Rat chamber system with IR translucent top (30cm X 40 cm light chamber;

20cm X 40cm dark chamber) o Compa ble with GigE camera recording o Compa ble with EthoVision XT video recording and behavioral tracking so ware to monitor ac vity within; 24hour monitoring op on x1 - 8 Unit Ac vity set up PhenoTyper 4500 system o Onsite set up includes digital camera, water bo le/feeder wall, running wheel, rat shelter, and associated mounts o Compa ble with EthoVision XT video recording and behavioral tracking so ware to monitor ac vity within; 24hour monitoring op on o Data collec on and analysis tools compa ble with Light Dark Box for reduced variability between analysis of different behavioral phenotypes

4 years of maintenance package for behavioral so ware, GigE IR cameras, PhenoTyper 4500, and

Light Dark Box system o comprehensive & integrated package to reduce use of GOV me, and cost of numerous calibra on site visit with mul ple companies

Onsite training

Shipment, delivery, and installa on at Aberdeen Pricing Ground, MD by 31 December 2024.