PacBio Requirements.pdf

Pacific Biosciences Sequel and Sequel II Systems Equipment, Maintenance and Services List

Application-specific Accessory Kits

Product Family Part Number Product Name / Description

Sequel, Sequel II 101-737-500 Iso-Seq Express Oligo Kit

The Iso Seq Express Oligo Kit contains two oligonucleotides to support the Iso Seq

Express workflow. The oligonucleotides are the PacBio Template Switching Oligo (TSO) and PacBio cDNA PCR primer. The oligos are designed to replace the equivalent oligos in the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module.

The reagents are sufficient for approximately 24 reactions.

Sequel 101-788-900 No-Amp Accessory Kit

No-Amp Accessory Kit contains Annealing Buffer, Sequencing Primer v4, and 10X Primer

Buffer v2. Annealing buffer is to be used during SMRTbell Adapter hairpin annealing. Sequencing Primer v4 and 10X Primer Buffer v2 is used to anneal

Sequencing Primer v4to final SMRTbell templates

Template Preparation Kits

Sequel, Sequel II 101-843-100 SMRTbell ExpTPK 2 + Enz Clean Up + SPv2

SMRTbell Express Template Prep Kit 2.0, Enzyme Clean Up Kit and Sequencing Primer v2 is a bundle kit of SMRTbell Express Template Preparation Kit 2.0, SMRTbell Enzyme

Clean Up Kit and Sequencing Primer v2. Each kit contains sufficient reagents to perform18 standard reactions.

Sequel, Sequel II 100-938-900 SMRTbell Express Template Prep Kit 2.0

The SMRTbell Express Template Prep Kit 2.0 contains sufficient reagents to prepare

18, 48, and 96 reactions to respectively support large-insert and full-length transcriptome libraries, microbial multiplexing, and amplicon sequencing. The kit is designed for diffusion loading only.

Sequel, Sequel II 100-991-900 SMRTbell Template Prep Kit 1.0-SPv3

SMRTbell Template Prep kit 1.0 with Sequencing Primer v3. The SMRTbell Template Prep

Kit 1.0-SPv3 contains reagent supplies to perform SMRTbell library preparations of primer-annealed SMRTbell libraries for insert sizes ranging from 500 bp to over 20

kb. Reagent quantities support 10 library preparations. This kit includes both

Sequencing Primer v2 for use with PacBio RS II and Sequencing Primer v3 for use with

Sequel System. Each primer supports 10 primer annealing reactions.

Sequel, Sequel II 100-992-200 SMRTbell Damage Repair Kit-SPv3

The SMRTbell Damage Repair Kit-SPv3 contains reagent supplies to perform DNA Damage

Repair, Exonuclease III and VII treatments, and primer- annealing of SMRTbell libraries for insert sizes ranging from 500 bp to over 20 kb. Reagent quantities support 10 library preparations. To be used with SMRTbell Barcoded Adapter Complete

Prep Kit to generate primer-annealed SMRTbell libraries ready for polymerase binding or to be used independently for extra DNA Damage Repair of template. This kit includes both Sequencing Primer v2 for use with PacBio RS II and Sequencing Primer v3 for use with Sequel System. Each primer supports 10 primer annealing reactions.

Sequel, Sequel II 101-633-500 Elution Buffer

The Elution Buffer (50 mL) is used with the SMRTbell library template preparation and barcoding associated kits for workflows requiring additional elution buffer and

AMPure clean-up steps.

Sequel, Sequel II 100-970-100 Sequencing Primer v3

Sequencing Primer v3 is used to anneal to SMRTbell templates as an alternate to

Sequencing Primer v2. Sequencing Primer v3 is compatible with SMRTbell templates and should only be used for SMRTbell libraries for diffusion and MagBead loading on the

Sequel System.

Sequel, Sequel II 101-847-900 Sequencing Primer v2

Sequencing Primer v2 is alternate sequencing primer used to anneal to SMRTbell templates generated with the SMRTbell Express Template Prep Kit 2.0

Barcoding and Associated Template Prep Kits

Sequel, Sequel II 101-628-400 Barcoded Overhang Adapter Kit- 8A

The Barcoded Overhang Adapter Kit – 8A contains 8 barcoded overhang adapters compatible with SMRTbell Express Template Prep Kit 2.0. The kit contains sufficient reagents to perform 6 ligation reaction for each barcoded overhang adapter to support multiplexed microbial genome sequencing, or 12 reactions for amplicon sequencing. The specific barcodes contained in this kit are: bc1001, bc1002, bc1003, bc1008, bc1009, bc1010, bc1011, bc1012.

Sequel, Sequel II 101-628-500 Barcoded Overhang Adapter Kit- 8B

The Barcoded Overhang Adapter Kit – 8B contains 8 barcoded overhang adapters compatible with SMRTbell Express Template Prep Kit 2.0. The kit contains sufficient reagents to perform 6 ligation reaction for each barcoded overhang adapter to support multiplexed microbial genome sequencing, or 12 reactions for amplicon sequencing. The specific barcodes contained in this kit are: bc1015, bc1016, bc1017, bc1018, bc1019, bc1020, bc1021, bc1022.

Sequel 101-629-100 Bar. Univ. F/R Primers Plate-96v2

Barcoded Universal F/R Primers Plate – 96 v2 is a 96-well plate containing barcoded primers. Each well on the plate contains a primer pair consisting of forward and reverse primers having the same barcode. Each barcode is present in only one well for a total of 96 different barcodes on the plate. Each primer has a universal sequence on the 3' end to allow for incorporation of barcodes into templates through a two-step PCR strategy. Reagent quantities support a single use of each of the 96 primer pairs in library preparations. The barcode sequences have been selected for optimized demultiplexing on both the Sequel System and PacBio RS II System.

Sequel, Sequel II 100-466-100 Barcoded Universal F/R Primers Plate -96

Barcoded Universal F/R Primers Plate - 96, is a 96-well plate containing barcoded primers. Each well on the plate contains a primer pair consisting of forward and reverse primers having the same barcode. Each barcode is present in only one well for a total of 96 different barcodes on the plate. Each primer has a universal sequence on the 3' end. Reagent quantities support a single use of each of the 96 primers pairs in library preparations.

Sequel, Sequel II 100-514-900 SMRTbell Barcoded Adapt Complete Prep-96

The SMRTbell Barcoded Adapter Complete Prep Kit -96 contains both SMRTbell Barcoded

Adapter Prep Kit and Barcoded Adapter Plate - 96. Reagent quantities support 96 complete Barcoded Adapter End Repair and Ligation reactions. User with SMRTbell

Damage Repair Kit to complete template preparation and yield primer-annealed

SMRTbell libraries.

Sequel, Sequel II 100-466-000 Barcoded Adapter Plate - 96

Barcoded Adapter Plate - 96, contains 96 unique barcoded adapters. Reagent quantities support a single use of each 96 barcoded adapter in library preparations.

Sequel, Sequel II 100-465-800 SMRTbell Barcoded Adapter Prep Kit

The SMRTbell Barcoded Adapter Prep Kit contains reagent supplies for the end repair of templates and ligation of SMRTbell barcoded adapters. Reagent quantities support

96 reactions.

Template and DNA/Polymerase Complex Clean Up Kits

Sequel, Sequel II 100-265-900 Ampure PB, 5 mLs

AMPure PB magnetic beads are specifically formulated for the Pacific Biosciences

SMRT sequencing workflow. Beads are used for DNA cleanup and purification during sample extraction and at various stages in the workflow. Reagent quantity supplied at 5 mLvolume.

Sequel 100-715-300 Loading Cleanup Bead Kit (10 rxn)

The Loading Cleanup Bead Kit (10 rxn) contains reagents to remove excess polymerase from a polymerase binding reaction. The Loading Cleanup Beads are only for diffusion loaded samples onto the Sequel SMRT Cell 1M. Reagent quantities support cleaning up

10 polymerase binding reactions.

Sequel, Sequel II 101-746-400 SMRTbell Enzyme Clean up Kit

The SMRTbell Enzyme Clean Up Kit contains 4 enzymes and buffer. The kit is designed to reduce the templates molecules that are not intact, circular SMRTbell templates.

The kit contains sufficient reagents to perform 18 reactions.

Binding Kits

Sequel 101-626-600 Sequel Binding and Internal Ctrl Kit 3.0

The Sequel Binding and Internal Cntrl Kit 3.0 is a bundle kit of Sequel Binding Kit

3.0 and Sequel DNA Internal Control Complex 3.0. The Sequel Binding Kit 3.0 contains reagent supplies to bind prepared DNA template libraries to the Sequel Polymerase

3.0in preparation for sequencing on the Sequel System. The result is a DNA polymerase/template complex. Sequel Polymerase 3.0 should be used only with Sequel

Sequencing Kit 3.0. Reagent quantities support 24 binding reactions and up to 70

SMRT cell runs depend on sample size and concentration. Sequel DNA Internal Control

Complex 3.0 contains a fixed template of 2 kb length, bound to Sequel DNA Polymerase

3.0 for use as an internal sequencing control on the Sequel System. Reagent quantities provide spike-in ccontrols for a minimum of 24 samples.

Sequel II 101-731-100 Sequel II Binding and Int Ctrl Kit 1.0

The Sequel II Binding and Internal Control Kit 1.0 is a bundle kit of Sequel II

Binding Kit 1.0 and Sequel II DNA Internal Control Complex 1.0. The Sequel II

Binding Kit 1.0 contains reagent supplies to bind prepared DNA template libraries to the Sequel II Polymerase 1.0 in preparation for sequencing on the Sequel II System.

The result is a DNA polymerase/template complex. Sequel Polymerase 1.0 should be used only with Sequel Sequencing Kit 1.0. Reagent quantities support 24 binding reactions and up to ~70 SMRT Cell 8M depending on use case, sample size and concentration. Sequel II DNA Internal Control Complex 1.0 contains a fixed template of 2 kb length, bound to Sequel II DNA Polymerase 1.0 for use as an internal sequencing control on the Sequel II Sysstem. Reagent quantities provide spike-in controls for a minimum of 24 samples.

Sequel II 101-842-900 Sequel II Bind Kit 2.0 and Int Ctrl 1.0

The Sequel II Binding Kit 2.0 and Internal Control Kit 1.0 is a bundle kit of Sequel

II Binding Kit 2.0 and Sequel II DNA Internal Control Complex 1.0. The Sequel II

Binding Kit 2.0 contains reagent supplies to bind prepared DNA template libraries to theSequel II Polymerase 2.0 in preparation for sequencing on the Sequel II System.

Sequel II Binding Kit 2.0 is to be used for all samples except for samples with short inserts <3 kb such as amplicons. The result is a DNA polymerase/template complex. SequelPolymerase 2. 0 should be used only with Sequel II Sequencing Kit

2.0. Reagent quantities support 24 binding reactions and up to ~70 SMRT Cell 8M depending on use case, sample size and concentration. Sequel II DNA Internal Control

Complex 1.0 contains a ffixed template of 2 kb length, bound to Sequel II DNA

Polymerase 1.0 for use as an internal sequencing control on the Sequel II System.

Reagent quantities provide spike-in controls for a minimum of 24 sample

Sequel II 101-843-000 Sequel II Bind Kit 2.1 and Int Ctrl 1.0

The Sequel II Binding Kit 2.1 and Internal Control Kit 1.0 is a bundle kit of Sequel

II Binding Kit 2.1 and Sequel II DNA Internal Control Complex 1.0. The Sequel II

Binding Kit 2.1 contains reagent supplies to bind prepared DNA template libraries to theSequel II Polymerase 2.1 in preparation for sequencing on the Sequel II System.

Sequel II Binding Kit 2.1 is to be used for samples with inserts < 3kb such as amplicons. The result is a DNA polymerase/template complex. Sequel Polymerase 2.1 should be used only with Sequel II Sequencing Kit 2.0. Reagent quantities support 24 binding reactions and up to ~70 SMRT Cell 8M depending on use case, sample size and concentration. Sequel II DNA Internal Control Complex 1.0 contains a fixed template of 2 kb length,, bound to Sequel II DNA Polymerase 1.0 for use as an internal sequencing control on the Sequel II System. Reagent quantities provide spike-in controls for a minimum of 24 samples.

SMRT Cell Loading Kits

Sequel, Sequel II 101-046-400 MagBead Binding Buffer Kit v2

The MagBead Binding Buffer Kit v2 has two primary uses: 1) It is used with the

MagBead Wash Buffer Kit and MagBeads using the Pacific Biosciences Binding

Calculator or SMRT Link Sample Set Up for the PacBio RS II to bind the

DNA/Polymerase complex and; 2) It is used to purify DNA/polymerase complexes after binding of polymerases in the Sequel Systems workflows.

Sequencing Kits

Sequel 101-597-900 Sequel Sequencing Kit 3.0 (4 rxn)

The Sequel Sequencing Kit 3.0 (4 rxn) contains a sequencing reagent plate with

Sequel Sequencing chemistry 3.0 and on-plate Sequel Oxygen Scavenging (OS) Enzyme, for SMRT Sequencing on the Sequel System. Reagent quantities support 4 sequencing reactions.The Sequel Sequencing Kit 3.0 is to be used with a Sequel SMRT Cell 1M v3.

Sequel Sequencing Kit 3.0 is compatible with templates bound to Sequel DNA

Polymerase 3.0.

Sequel 101-597-800 Sequel Sequencing Kit 3.0 (8 rxn)

The Sequel Sequencing Kit 3.0 (8 rxn) contains a sequencing reagent plate with

Sequel Sequencing chemistry 3.0 and on-plate Sequel Oxygen Scavenging (OS) Enzyme for SMRT Sequencing on the Sequel System. Reagent quantities support 8 sequencing reactions. The Sequel Sequencing Kit 3.0 is to be used with a Sequel SMRT Cell 1M v3. Sequel Sequencing Kit 3.0 is compatible with templates bound to Sequel DNA

Polymerase 3.0.

Sequel 101-642-400 Sequel Sequencing Kit 3.0 Bundle (4 rxn)

The Sequel Sequencing 3.0 Bundle (4 rxn - 5 pack) contains reagent and disposable supplies for 20 reactions of SMRT Sequencing. This bundle includes: 5 Sequel

Sequencing Kit 3.0 (4 rxn), 1 Sequel SMRT Cell Oil pack, and 1 Tube Septa pack.

Sequel 101-642-300 Sequel Sequencing Kit 3.0 Bundle (8 rxn)

The Sequel Sequencing 3.0 Bundle (8 rxn - 5 pack) contains reagent and disposable supplies for 40 reactions of SMRT Sequencing. This bundle contains 5 Sequel

Sequencing Kit 3.0 (8rxn), 1 Sequel SMRT Cell Oil pack, and 1 Tube Septa pack.

Sequel II 101-717-200 Sequel II Sequencing Kit 1.0

The Sequel II Sequencing Kit 1.0 (4 rxn) contains a sequencing reagent plate with

Sequel II Sequencing chemistry 1.0 and on-plate Sequel Oxygen Scavenging (OS)

Enzyme, for SMRT Sequencing on the Sequel II System. Reagent quantities support 4 sequencing reactions. The Sequel II Sequencing Kit 1.0 is to be used with a Sequel

II SMRT Cell 8M. Sequel II Sequencing Kit 1.0 is compatible with templates bound to

Sequel II DNA Polymerase 1.0.

Sequel II 101-768-000 Sequel II Sequencing Kit 1.0 Bdle (5-pk)

The Sequel II Sequencing 1.0 Bundle (4 rxn - 5 pack) contains reagent and disposable supplies for 20 reactions of SMRT Sequencing. This bundle includes: 5 Sequel II

Sequencing Kit 1.0 (4 rxn), 1 Sequel SMRT Cell Oil pack, and 1 Tube Septa pack.

Sequel II 101-820-200 Sequel II Sequencing Kit 2.0 (4 rxn)

Sequel II Sequencing Kit 2.0 (4 rxn) contains a sequencing reagent plate with Sequel

II Sequencing Chemistry 2.0 and on-plate Sequel Oxygen Scavenging (OS) Enzyme, for

SMRT Sequencing on the Sequel II System.

Sequel II 101-849-000 Sequel II Sequencing Kit 2.0 Bdle (5-pk)

The Sequel II Sequencing 2.0 Bundle (4 rxn - 5 pack) contains reagent and disposable supplies for 20 reactions of SMRT Sequencing. This bundle includes: 5 Sequel II

Sequencing Kit 2.0 (4 rxn), 1 Sequel SMRT Cell Oil pack, and 1 Tube Septa pack.

SMRT Cells

Sequel 101-531-000 SMRT Cell 1M v3 Tray

The Sequel SMRT Cell 1M v3 (4/tray) is a set of 4 nanofabricated consumables each patterned with 1,000,000 wells called zero-mode waveguides (ZMWs). They are packaged together in a streamlined 4 SMRT Cell format (4/tray). SMRT Cell 1M v3 is compatible with Sequel Binding Kit 3.0 and Sequel Sequencing Kit 3.0. One SMRT Cell is utilized in each sequencing reaction, and experiments can be run on a single or in batch mode, giving your projects a greater level of flexibility. The SMRT Cell 1M v3 can acquire sequencing data up to 10 hours of collection time.

Sequel 101-531-001 SMRT Cell 1M v3 LR Tray

The Sequel SMRT Cell 1M v3 LR Tray contains 4 SMRT Cells. Each is a nanofabricated consumable patterned with 1,000,000 wells called zero-mode waveguides (ZMWs). The

SMRT Cell 1M v3 LR is utilized for sequencing collection times up to 20 hours. One

SMRT Cell is utilized for each sequencing reaction.

Sequel II 101-389-001 SMRT Cell 8M Tray

The SMRT Cell 8M Tray contains 4 SMRT Cells to be used with the Sequel II System.

Each is a nanofabricated consumable patterned with 8,000,000 wells called zero-mode waveguides (ZMWs). One SMRT Cell is utilized for each sequencing reaction. One SMRT

Cellis utilized in each sequencing reaction, and experiments can be run on a single or in batch mode, giving your projects a greater level of flexibility

Sequencing Controls and Accessories

Sequel 101-500-300 Sequel DNA Internal Control Complex 3.0

Sequel DNA Internal Control Complex 3.0 contains a fixed template of 2 kb length, bound to Sequel DNA Polymerase 3.0 for use as an internal sequencing control on the

Sequel System. Reagent quantities provide spike-in controls for a minimum of 24 samples.

Sequel II 101-717-600 Sequel II DNA Internal Cntrl Complex 1.0

Sequel II DNA Internal Control Complex 1.0 contains a fixed template of 2 kb length, bound to Sequel II DNA Polymerase 1.0 for use as an internal sequencing control on the Sequel II System. Reagent quantities provide spike-in controls for a minimum of

24samples.

Sequel, Sequel II 100-621-300 Sequel SMRT Cell Oil

The Sequel SMRT Cell Oil contains 5 reagent tubes for SMRT Sequencing on the Sequel

System (used with the Sequel Sequencing Kit). Reagent quantities support 5 sequencing plates.

Plastic Consumables and Labware

Sequel, Sequel II 100-667-700 Tube Septa (10 pack)

One bag of 10 tube septa approved for evaporation control of Oxygen Scavenging (OS)

Enzyme and SMRT Cell Oil tubes on the PacBio Systems.

Sequel, Sequel II 100-667-400 Sequel Sample Plate Foil (100 per pack)

One case of 100 foil seals approved for evaporation and environmental control contained in the Sequel Sample Plate. The foil seals are heat-treated to cover the

Sequel Sample Plate.

Sequel, Sequel II 000-448-888 Sample Plate (50 plates)

1 case of 96-well PCR plates approved for sample placement on the PacBio Systems.

Each case contains 50 plates.

Sequel, Sequel II 100-667-500 Sequel Mixing Plate (120 per pack)

One case of 96-well deep-well plates contains 120 plates approved for reagent and sample mixing on the Sequel System.

Sequel, Sequel II 100-667-601 Sequel Pipet Tips v2 (40 racks/case)

Sequel Pipet Tips v2 have tip rack which helps secure the tip rack to the tip box.

It contains filtered tips approved for robotic liquid handling on the Sequel System.

Each case contains 40 racks of 96 tips each.

Training

Sequel, Sequel II 100-125-100 On-site System Training (3 Days)

On-site instruction of up to three (3) participants at the customer's location by a

Pacific Biosciences Field Applications Specialist (FAS). Includes training kit and

FAS travel-related expenses. Training includes Assay, Software and Bioinformatics.

Sequel, Sequel II 101-769-700 On-Site Bioinformatics Training (1 Day)

On site instruction of up to five (5) participants at the customer’s location by a

Pacific Biosciences Bioinformatics Field Application Specialist. Includes hands-on training and FAS-related expenses. Training will cover an introduction and overview of PacBio provided informatics solutions. Additionally, the customer can choose in depth discussion and instruction for up to three (3) applications, including but not limited to in-depth algorithm instruction, implementation recommendations, and command lineinstruction on running analyses of previously generated data (data not provided by PacBio). Please note that the customer is responsible for arranging terminal and SMRTLink access to FAS for training. No IT support will be provided in this training.

Standard Service Contracts

Sequel 101-101-100 Prem. Sequel Service Contract, w Insight

Premier Sequel Service Contract with Insight The Premier Sequel Service Contract plan covers all parts, labor, and consumables required for the repair and recommended preventative maintenance with an OQ of an installed system with priority service. Includes OQ with qualified repairs.Subject to Pacific Biosciences Standard

Terms and Conditions and applicable Service Contract Data Sheet.

Sequel 101-101-200 Prem. Sequel Serv. Contract, w/o Insight

Premier Sequel Service Contract without Insight The Premier Sequel Service Contract plan covers all parts, labor, and consumables required for the repair and recommended preventative maintenance with an OQ of an installed system with priority service. Includes OQ with qualified repairs. Subject to Pacific Biosciences

Standard Terms and Conditions and applicable Service Contract Data Sheet.

Sequel 100-715-500 Comp. Sequel Service Contract, w Insight

Comprehensive PacBio Sequel Service Contract with Insight. The Comprehensive PacBio

Service Contract plan covers all parts, labor, and consumables required for the repair and recommended preventative maintenance of an installed system with priority service. Subject to Pacific Biosciences Standard Terms and Conditions and applicable Service Contract Data Sheet.

Sequel 100-715-600 Comp. Sequel Serv. Contract, w/o Insight

Comprehensive PacBio Sequel Service Contract without Insight. The Comprehensive

PacBio Sequel Service Contract plan covers all parts, labor, and consumables required for the repair and recommended preventative maintenance of an installed system with priority service. Subject to Pacific Biosciences Standard Terms and

Conditions and applicable Service Contract Data Sheet.

Sequel II 101-711-000 Prem Sequel II Srv Contract, w Insight

Premier Sequel™ II Service Contract with Insight The Premier Sequel II Service

Contract plan covers all parts, labor, and consumables required for the repair and recommended preventative maintenance with an OQ of an installed system with priority service. Includes OQ with qualified repairs. Subject to Pacific Biosciences

Standard Terms and Conditions and applicable Service Contract Data Sheet.

Sequel II 101-711-100 Prem Sequel II Srv Contract, w/o Insight

Premier Sequel™ II Service Contract without Insight The Premier Sequel II Service

Contract plan covers all parts, labor, and consumables required for the repair and recommended preventative maintenance with an OQ of an installed system with priority service. Includes OQ with qualified repairs. Subject to Pacific Biosciences

Standard Terms and Conditions and applicable Service Contract Data Sheet.

Sequel II 101-711-200 Comp. Sequel II Srv Contract, w Insight

Comprehensive Sequel™ II Service Contract with Insight Covers all parts, labor, and consumables required for the repair and recommended preventative maintenance of an installed system with priority service. Subject to Pacific Biosciences Standard

Terms and Conditions and applicable Service Contract Data Sheet.

Sequel II 101-711-300 Comp. Sequel II Srv Contract w/o Insight

Comprehensive Sequel™ II Service Contract without Insight Covers all parts, labor, and consumables required for the repair and recommended preventative maintenance of an installed system with priority service. Subject to Pacific Biosciences Standard

Terms and Conditions and applicable Service Contract Data Sheet.

Laboratory Services

Sequel 101-101-300 Sequel System Inst and Oper Qual (IQOQ)

Sequel Installation and Operational Qualification (IQOQ) The Sequel System

Instrument Qualification (IQ) demonstrates that the Sequel System has been delivered and installed in accordance with pre-determined specifications and safety regulations. An Operational Qualification verifies the functionality of Sequel

System based on manufacturing guidelines and specifications. Sequel IQOQ includes an automatic upgrade to Premium Service. Note: IQ only performed when installing or relocating a Sequel System.

Sequel II 101-711-500 Sequel II System Inst & Oper Qual (IQOQ)

Sequel II Installation and Operational Qualification (IQOQ) The Sequel II System

Instrument Qualification (IQ) demonstrates that the Sequel II System has been delivered and installed in accordance with pre-determined specifications and safety regulations. An Operational Qualification verifies the functionality of Sequel II

System based on manufacturing guidelines and specifications. Sequel II IQOQ includes an automatic upgrade to Premium Service. Note: IQ only performed when installing or relocating a Sequel II System.

Accessory Third Party Equipment - Optional

Sequel, Sequel II 100-609-000 UPS, 208V, 5000VA, 50-60 Hz, UL (Sequel)

UPS, 208V, 5000VA, 50/60 Hz, UL (Sequel and Sequel II). Instrument Grade UPS for use in the USA and Canada with Sequel or Sequel II System. 208 V, 50/60 Hz. The North

American UPS purchased from Pacbio is pre-configured and will be installed at the timeof the Instrument Installation.

Consumable Starter Pack

Sequel 101-645-000 Sequel Consumable Starter Kit 3.0

Starter bundle that provides a sampling of consumables to start running the Sequel

System. includes: Template Preparation, Sequel Binding Kit 3.0, Sequencing Kit 3.0, Internal Control, SMRT Cell 1M v3, MagBead Binding Buffer Kit v2, tips, and plastics consumables. The starter kit contains core reagents to run ~16 SMRT Cell 1Ms v3.

Other consumables may have fewer units (e.g. Template prep) or more units (e.g. tips and plates).

Sequel II 101-753-300 Sequel II Sys Consumable Starter Kit 2.0

The Sequel II System Consumable Starter 2.0 bundle provides a sampling of consumables to start running the Sequel II System and includes: SMRTbell Express

Template Preparation Kit 2.0, Sequel II Binding Kit 2.0, Sequel II Sequencing Kit

2.0, Sequel II Internal Control 1.0, SMRT Cell 8M, MagBead Binding Buffer Kit v2, AMPure PB, tips, and plastics consumables. The Consumable Starter Kit contains core reagents to run ~eight SMRT Cells 8M. Other consumables may have fewer units (e.g.

Template prep) or moreunits (e.g. tips and plates).

System Upgrades

Sequel, Sequel II 101-691-500 Sequel System 8M Upgrade

Sequel System 8M Upgrade, includes 3 months limited service extension