Equipment_Request.pdf

The following items shall be supplied:

Qty Item No Name Description

1 SPRCH-

01006 Purchase BAC from

CHORI

BAC: RP23-8P16

1 SPRCH-

01006

Purchase BAC from

CHORI

BAC: RP23-12E18

1 STKMU-

02001

TurboKnockout® Conditional Knockout Vector Construction

Turnaround time: 8-14 weeks.

Deliverable: TurboKnockout® Gene Targeting Report.

Design and construction of TurboKnockout® conditional knockout vector after client approval of Cyagen-proposed TurboKnockout® gene targeting strategy.

1 STKMU-

02002

TurboKnockout® Conditional Knockout Targeting in ES Cells

Turnaround time: 8-14 weeks.

Deliverable: Correctly Targeted and Verified ES Cell Genotyping Report.

TurboKnockout® Vector will be purified, linearized, and electroporated into ES cells. ES cells are subjected to drug selection, screened by long range PCR, and verified for correct gene targeting by Southern blot.

2 STKMU-

02005

TurboKnockout® Conditional Knockout Karyotype ES Cells.

Turnaround time: 1-2 weeks.

Chromosome counting is performed to ensure ES cells have the correct number of chromosomes.

1 STKMU-

02003

TurboKnockout® Conditional Knockout Mouse Generation

Turnaround time: 8-12 weeks.

Deliverable: A minimum of 3 TurboKnockout® heterozygous mutant mice

1 BCMU-01005 ESC conditional KOKI breeding, tissue-specific

Turnaround time: 5-9 months Breed with Jax Strain #J005657

1 Handling Project Handling Fee