Specifications.pdf

Specifications: Multiphoton Microscopy System

Delivery Date: Within 16 weeks of receipt of order.

Delivery Location:

National Eye Institute (NEI) Building 35, Room 3F226 Bethesda, MD 20892

Brand Requirement: Scientifica

Item Description Qty

1 SliceScope with PatchPad - Multiphoton Compatible 1.00 SliceScope upright microscope with motorised frame. Highly mechanically stable device with a thinner profile. Comes complete with motorised positioning of objective and condenser in Z axis, controlled by Scientifica PatchPad as well as LinLab software. Does not include any other Olympus optical or mechanical assemblies which have to be specified and ordered separately. Does not include cameras, condensers, light sources or objectives. Accepts Scientifica multiphoton scan head MP-1000.

2 MMBP Stand Alone 1.00 MMBP Stand Alone. Low profile stage with one motorised stage and one slave. No

Rack, Drive Card or Control interface included.

3 Post and Platform for MMBP 2.00 Post and Platform, typically used for mounting the MicroStar onto the

Scientifica MMBP

4 Double MicroStar with Control Cube

Two motorised 3 axis super smooth micromanipulators providing 20 mm of high resolution (20 nm) travel in XZ and 14mm in Y and 25mm travel in fourth virtual approach axis. Drift of less than 1 micron over two hours. Extremely low electrical noise. Mounting hardware, vertical rotation stage, high precision rotary sensor for angle detection and sliding carriage for headstage. Also includes switchable Control Cube and LinLab software for set up, PC control & custom settings.

1.00

5 Scientifica Control cube and cable 1.00 Scientifica Control cube and cable 6 780nm LED System for SliceScope 1.00 Includes: 780nm LED module to attach to transmitted illumination port on

Scientifica SliceScope, handset with illuminated on/off switch, intensity control knob, power supply 90-250V. Suitable for IR DIC, Dodt contrast, oblique illumination.

7 Condenser 1.00 Achromatic/Aplanatic condenser, with numerical aperture of 1.40

8 Contrast Tube 1.00 Contrast tube to allow the visualisation of cells in thick tissue. For best results use in combination with an oil- immersion condenser.

9 Immersion oil 8cc 1.00 Immersion oil 8cc (inc when quoting for the U-AAC condenser (2P)

10 20x Objective 1.00 XL U M Plan Fluorite 20x, WD 2.0mm, NA 1, CC=_ (water), FN22 at eyepiece, mounting thread: M25x0.75, parfocal distance: 75mm

11 MultiPhoton Objective Slider and Bracket 1.00 A set of objective adapters (RMS, extended RMS, M27 and M25 threaded objectives), two sliders and sliding rails, positioning pins and bracket. Does not include DIC prism pocket

12 SliceScope Fluorescence Adapter Kit 1.00 To attach the Olympus RFA or URA to the Scientifica SliceScope

13 Fluorescence Turret 1.00 BX reflected illuminator, turret for 6 fluorescence cubes, exchangeable and centerable aperture stop and field stop, 3 slots for ND and excitation balancer, sliders

UV protective shield.

14 Dual Adapt 1.00 Adapter to fit two fluorescent light sources to a microscope. Please specify which microscope will be used:

15 Optogenetic Scan Head Mirror 1.00 Shortpass Dichroic Mirror with Extended Reflection 16 Tube Lens 1.00 Single port tube for infrared applications, accepts UIS photo/ video accessories

17 Adapter Tube 1.00 Direct Image Video Adapter Tube 1x 18 Camera Mount 1.00 C-Mount Adapter Ring 19 Multiphoton Alignment Pack 1.00 The pack of alignment tools are required to ensure proper aligment of the laser through the Scientifica multiphoton scan head which is requred for optimal system performance. Includes CCD camera, crosshair, grounded glass disk, viewing card and tube lens)

20 Multiphoton Filter Set 2.00 Multiphoton filter set to separate green and red emitting dyes. 525/50nm and

620/60nm emission filters with 565LP dichroic included.

21 Pockel cell for laser attenuation of multiphoton lasers 1.00 Pockel cell for laser attenuation of multiphoton lasers. Includes Pockel cell, driver electronics, adjustable mount.

22 Multiphoton Resonant Scan Head (8kHz) 1.00 Scientifica Multiphoton resonant scanning system consists of a separated X resonant galvonometer (8kHz) and Y galvonometers with 4mm mirrors. The system is designed to obtain diffraction limited resolution with large back aperture objectives (=20mm). The scan head is compatible with the Scientifica SliceScope. To be controlled with open source freeware. Not Included: SliceScope, Launch Optics, Collection Optics, data acquisition etc.

23 Workstation and DAQ package for resonant scanning – SciScan and ScanImage5 compatible

1.00

Workstation including Quad Core PC and peripherals. PXI- based data acquisition boards for resonant scanning control and image acquisition Depending on the acquisition software to be used, the end-user is required to have the appropriate Labview or Matlab licences. These are not included with the system.

24 Laser Scanning Contrast for Resonant System 1.00 Laser scanning contrast for resonant multiphoton allows the generation of an IR contrast image. Requires Contrast Tube and oil-condenser.

25 Custom Port for Visual Stimulus -775nm 1.00 A customised combiner port containing a custom sized 775nm Longpass dichroic mirror

26 ChromoFlex - Two channel PMT detection unit 1.00 ChromoFlex base package which includes Collection Module with large collection optics, 2m AR coated liquid light guide, two channel remote unit Detection Module populated with Hamamatsu H10770PA-40 GaAsp detectors.

Also includes two objective sliders, set of threaded adapter rings (M27, M25, RMS), PMT controller and remote light senor unit.

27 ChromoFlex - SliceScope mounting bracket 1.00 Mounting bracket to attach ChromoFlex Collection Module (included in MPLG-

1002) to the SliceScope

28 20MHz current preamplifier 2.00 Single channel 20MHz PMT current preamplifier.

29 Low noise preamplifier power supply 1.00 Low noise linear preamplifier power supply. Supports up to 4 x 4000-473 1MHz current preamplifier or 4 x 4000-391 20MHz current preamplifier.

30 BNC-to-BNC cable 2.00 BNC-to-BNC cable 31 Scientifica Laser Splitting Module 1.00 The module enables the laser beam to be split into two paths. Required if two multiphoton systems are to be used with one multiphoton laser.

32 Scientifica Installation and Training 1.00 Scientifica Installation and Training is charged at quoted rate plus expenses per person per day. If your equipment is being installed by Scientifica personnel please ensure that you have fully read the Scientifica Installation and Training Terms and Conditions which form part of this quotation.

Installation and Training

Unpacking, assembly and full build of the Scientifica products and Scientifica produced software.

Installation of the optical components, cameras and light sources onto the microscope and alignment where suitable.

Full product training on all Scientifica products as part of the installation.

An overview of the operation of the entire system and the supplied software.

Guidance and advice on the installation of third party products supplied by Scientifica.

Alignment of any table optics purchased from Scientifica Microscope laser alignment Training on how to carry out laser alignment Installation and calibration of Laser Scanning Software Training on Laser Scanning Software.

Site Acceptance Test:

Once the installation has been completed in accordance with the terms and conditions above the Engineer will discuss the installation with the customer and agree that the installation is complete. The Engineer will provide a Site Acceptance Test certificate which the customer will sign to confirm acceptance. Any anomalies or necessary corrective actions encountered during the installation will be noted on the Site Acceptance Test Certificate.

Post Installation Support:

Post installation support to be provided by telephone during normal business hours, as well as on-line videos and webinars. When necessary remote desk-topping to be arranged.

Acquisition Contact:

Eric D. Chapman Contract Specialist 301-435-3910 eric.chapman@nih.gov mailto:eric.chapman@nih.gov