SOW.pdf

STATEMENT OF WORK

BACKGROUND

The evaluation of the protein expression levels in autoimmune diseases like type I diabetes, will shed light into possible alterations in signaling pathways. The objective is to learn whether a disequilibrium inflammatory signaling pathways, under different biological stimuli, exists in Type 1 diabetes, and whether this knowledge will open the door for future treatments for this disease. We will evaluate a concise number of proteins, 13, involved in inflammatory pathways. The total protein levels will be evaluated from the supernatant of TruCulture tubes derived from type 1 diabetes patients and healthy controls. These TruCulture tubes were previously acquired, and by evaluating the protein levels using TruCulture Optimap provides with continuity, reagent compatibility and reproducibility in this project. The technology to accurately evaluate proteins from the supernatant of TruCulture tubes.

OBJECTIVE

Progression of autoimmune diseases like type 1 diabetes may involve the increased expression of proteins involved in inflammatory pathways. The evaluation of the protein levels of a selected group of proteins will help us get a better picture of their involvement or role in the disease. The signaling molecules, which are involved in the regulation of inflammation, are: Epithelial-derived neutrophil-activating protein 78, granulocyte-macrophage colony-stimulating factor, IFN-gamma, interleukin-10, interleukin-12 subunit p40, interleukin-13, interleukin-17, interleukin-1-beta, interleukin-2, interleukin-23, interleukin-23, interleukin-6, interleukin-8, and tumor necrosis factor alpha. This cohort of proteins (TruCulture OptiMAP) has been used with excellent results by our collaborators at the Pasteur Institute.

PERIOD OF PERFORMANCE

The expected completion of this experiments involved in this PO is approximately 30 days processing of the samples once received.

INSPECTION AND ACCEPTANCE

The Project Officer will perform inspection and acceptance of materials and services to be provided. Inspection and acceptance will be done AFTER All materials are unpacked, and confirm the data for operation. Receiving will be completed no later than 3 days following date of Delivery, and DOES NOT constitute acceptance and Delivery.

Acceptance and Delivery will be complete once the signed packing slip is provided to the Vendor.

Inspection and acceptance will be performed at the place of installation - National Institutes of Health, NIDDK, 9000 Rockville Pike, Maryland 20892.

PROJECT OFFICER

The following Project Officer will represent the Government for the purpose of this Purchase Order:

Project Officer: To be provided at time of award Alternate Project Officer: To be provided at time of award

The Project Officer is responsible for: (1) monitoring the Contractor's technical progress, including the surveillance and assessment of performance and recommending to the Contracting Officer changes in requirements; (2) interpreting the statement of work and any other technical performance requirements; (3) performing technical evaluation as required; (4) performing technical inspections and acceptances required by this Purchase Order; and (5) assisting in the resolution of technical problems encountered during performance.

The Contracting Officer is the only person with authority to act as agent of the Government under this Purchase Order. Only the Contracting Officer has authority to:

(1) direct or negotiate any changes in the statement of work; (2) modify or extend the period of performance; (3) change the delivery schedule; (4) authorize reimbursement to the Contractor any costs incurred during the performance of this Purchase Order; or

(5) otherwise change any terms and conditions of this Purchase Order.