Attachment 4 - Statement of Needs.pdf
PDF 239 KB Posted
- Attached to
- Next Generation Sequencing System and Support Plan Federal contract opportunity
- Solicitation number
- NIAID-SS-25-2265418
About this file
This Statement of Need outlines requirements for a Next Generation Sequencing System for a laboratory currently using Illumina instrumentation. The system must be a compact benchtop platform (less than 25in x 23in x 26in, weighing under 325lbs) capable of single-cell, whole-exome, and RNA sequencing. Key technical specifications include at least 14 configurations, read lengths from 1x50bp to 2x300bp, output of 10-540Gb per run, and the ability to generate 100M-1.8B single-end reads in 8-44 hours.
The system must offer a streamlined workflow with less than 10 minutes setup time, no wash cycle interruptions, and onboard data analysis in under two hours. It requires compatibility with a comprehensive ecosystem of 14+ library prep kits, supporting both cloud-based and on-premises options. Critical to the procurement is seamless integration with existing Illumina connections, operations, platforms, XLEAP-SBS™ reagent kits, and Respiratory Virus kit sets to ensure continuity of ongoing laboratory studies. The system should utilize patterned flow cell technology with high cluster density to maximize sequencing throughput and cost-efficiency.
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| File | Type | Posted |
|---|---|---|
| CSS_NIAID-SS-25-2265418.pdf |
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Statement of Need – Next Generation Sequencing System
This Statement of Need is for the procurement of a Next Generation Sequencing System and associated product bundle and software. The Next Generation Sequencing System shall provide access to a range of applications including single-cell, whole-exome, and RNA sequencing on a benchtop platform.
Size: Footprint must be less than 25in height x 23in width x 26in depth or smaller, allowing for benchtop or BSC use. The machine must weigh no more than 325lbs.
Capability: The system shall have a minimum of 14 configurations and read lengths from 1x50bp to 2x300bp or greater. It shall provide a minimum output of 10-540Gb at 100M – 1.8B single-end reads per run in 8-44 hours, with a minimum of four flow cell configurations.
The system must utilize patterned flow cell technology with the highest cluster density available on the market to maximize sequencing throughput and cost-eƯiciency. It shall employ advanced chemistry to deliver faster, higher quality, and more economical sequencing compared to standard SBS technologies. The system shall allow for access to applications including single-cell, whole-exome, and RNA sequencing.
Streamlined Workflow and Flexibility: The system shall require less than 10 minutes of for setup and the workflow shall not be interrupted by any wash cycles. Samples shall be tracked and runs must be capable of management via cloud-based and on-premises options. Data must be able to be analyzed onboard in under two hours.
Integrated Kits: The system shall integrate with a comprehensive ecosystem of 14 or more compatible library prep kits. These kits must employ both cloud-based and on-premises options and be capable of building libraries and generating scalable sequence generation.
Outputs must be exportable and able to be read with onboard secondary analysis.
Continuity: The laboratory section currently uses instrumentation from Illumina. The system shall be seamlessly compatible with the current connections, operation, platforms and capabilities as well as with the Illumina XLEAP-SBS™ reagent kits and Illumina Respiratory Virus kits Sets ensure the accuracy and continuity of ongoing studies.
Commented [SJ([1]: Even more so than the others, we have already invested significantly in the Illumina system. While the continuity of instrumentation for protocols/learning curve is not critical, the compatibility with Illumina kits is a critical component of the identification of this system. Please feel free to edit this as you see fit, or remove entirely if not suitable.
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