Attachment 4 - Statement of Needs.pdf
PDF 149 KB Posted
- Attached to
- Single cell RNA Sequencing System Federal contract opportunity
- Solicitation number
- NIAID-SS-25-2265393
About this file
This Statement of Need outlines requirements for a benchtop single-cell RNA sequencing system with specific technical and operational specifications. The system must have a compact size (less than 12in x 12in x 20in, weighing under 45lbs), high-throughput capabilities to process up to 2.5 million cells in a single 6-minute run, and support multiple sample types including fresh, frozen, and FFPE samples. Key requirements include on-chip multiplexing, cell recovery of up to 80%, flexible cell input (500-20k cells), custom probes for mouse and human samples, and a streamlined workflow completable in a single day.
The system must offer at least three integrated multi-omic assay kits covering whole transcriptome analysis, including gene expression, immune clonotype, chromatin accessibility, and protein detection. Additional mandatory features include wireless connectivity, cloud backup, comprehensive software with integrated analysis workflows, a 12-month warranty and service plan, training resources, and compatibility with existing 10x Genomics instrumentation and kits. The vendor must provide continuous software development, free upgrades, technical support, and resources such as user guides, protocols, and training to ensure seamless integration and user proficiency.
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| File | Type | Posted |
|---|---|---|
| CSS_NIAID-SS-25-2265393.pdf |
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Text version
Statement of Need – Single cell RNA Sequencing System
This Statement of Need is for the procurement of a benchtop single-cell RNA sequencing system and associated software and kits.
Size: The system shall have a small footprint of less than 12in height x 12in width x 20in depth, allowing for benchtop or BSC use. The machine shall weigh no more than 45lbs.
Capability: The high-throughput system shall eƯiciently partition and barcode up to 2.5 million fresh, frozen, or fixed cells in a single run. Runs shall be completed in 6 minutes or under, enhancing workflow eƯiciency. In addition to fresh and frozen samples, it shall also support a broad range of sample types including FFPE samples. The system shall support on-chip multiplexing without additional upstream tagging, and must provide eƯicient and reproducible cell recovery of up to 80% (reducing multiplet rates as compared to methods like combinatorial barcoding). An in-built microfluidics system shall enable large-scale analysis at less than 1 cent per cell, and input shall be flexible from 500 to 20k cells per sample. Custom probes shall accommodate analysis of sample types from both mice and humans.
Streamlined Workflow: The workflow should be able to be completed in a single day, half the time of manual methods. The platform shall include intuitive processing and visualization software with integrated analysis workflows, allowing rapid export of data and deep technical expertise.
Kit Integration and Cost: The system shall oƯer at least three integrated kits, which shall include a range of multi-omic assays for multiplex or customizable analysis. Assays shall provide whole transcriptome coverage and include gene expression, immune clonotype, chromatin accessibility, and cell surface and other protein detection platforms. Kits will include internal controls and normalization, and be designed to minimize handling and user errors via automated cell partitioning and barcoding.
Flexibility and Connectivity: The system shall provide wireless connectivity and integration with a cloud backup for real-time support and software updates. The system should provide various connectivity options including ethernet, Wi-fi, USB, and cloud access with no limit to the number of runs or scale of studies.
Maintenance and Training: The system shall include a 12-month warranty and a 12-month service insurance plan, with technicians and specialist available for any questions or resources. The system shall have the capability of providing training kits to allow for inexpensive instrument validation and practice, and tailored training sessions must be available to the user if needed. The vendor shall provide comprehensive resources for assistance, including user guides, protocols, how-to videos, and analysis guides. The system should provide continuous software development and free upgrades to allow for access to diƯerent assays and up-to-date performance.
Continuity: The laboratory section uses existing instrumentation from 10x Genomics. The system must be seamlessly compatible with 10x Genomic Kits, 10x Genomics GEM-X Universal Expression and Next GEM Single Cell Kits. To ensure the continuity of laboratory studies, the system must feature the same connections and capabilities.
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