AMBIS_1973527_-_FBO_Proposal_Quote.xlsx

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Metabolic Analyses - CHO Cell Characterization
Solicitation number
NIAID-RFQ19-1973527
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NIAID-RFQ19-1973527 - Quote Proposal

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Sheet1

NIAID-RFQ19-1973527
Quote Proposal
LineProject #Description of Service (quality-controlled data set); see NOTE for requirementQuantity (# samples)Quoted Pricing
1Project 14 samples (cell and supernatant) per clone taken at different time points during 14-day platform fed-batch process à 32 samples for 1 project, 8 sample groups32
2Project 24 samples (cell and supernatant) per clone taken at different time points during 14-day platform fed-batch process à 32 samples for 1 project, 8 sample groups32
3Project 2 MatrixC-Matrix to for merging potentialProject 1 and 2
4Project 3 (option)4 samples (cell and supernatant) per clone taken at different time points during 14-day platform fed-batch process à 32 samples for 1 project, 8 sample groups32
5Project 3 Matrix (option)C-Matrix to for merging potentialProject 1, 2 and 3
6Project 4 (option)4 samples (cell and supernatant) per clone taken at different time points during 14-day platform fed-batch process à 32 samples for 1 project, 8 sample groups32
7Project 4 Matrix (option)C-Matrix to for merging potentialProject 1,2, 3 and 4
8Project 5 (option)4 samples (cell and supernatant) per clone taken at different time points during 14-day platform fed-batch process à 32 samples for 1 project, 8 sample groups32
9Project 5 Matrix (option)C-Matrix to for merging potentialProject 1,2,3,4 and 5
TOTAL
NOTE: The VPPL requires the service of metabolic analysis of 4 samples (cell and supernatant) per clone taken at different time points during a 14-day platform fed-batch process. There will be 32 samples for project. The VPPL requires 2 projects with the option of 3 additional projects. Samples will be provided by the government. Results are to be delivered in a data table (excel format) listing every structurally named metabolite identified and the relative abundance of each metabolite. The status of metabolic pathways such as Glycolysis, Pentose Phosphate Pathway, Redox Pathway and Glycosylation Pathways for each cell line must also be provided. Furthermore, a graphical representation of the trends for each metabolite with time within the pathway is required. Data shall be statistically analyzed by a seasoned statistician and biochemical interpretation of significant findings shall be performed by a PhD-level biologist trained in metabolomics.

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