SOW DNA samples Feb 12 2020.pdf

Statement of Work Acroporid Coral Tissue DNA Extraction/Analysis

Scope of Work

The Awardee shall have the primary responsibility for implementing the following activities:

Task 1: Extract DNA from a maximum of 480 samples of Acropora palmata or A.

cervicornis tissue samples.

Task 2: Conduct genotypic analysis of extracted samples using Coral Axiom Chip mini array.

These samples will be collected by the National Marine Fisheries Service (NMFS)/Southeast Fisheries Science Center (SEFSC), preserved in 96% molecular grade ethanol and sent to the Contractor at least two months prior to the end date of the award for analysis. Should genotyping fail for a given sample, PCR and genotyping will be repeated once. If still no results can be obtained, this sample will be regarded as failed due to poor preservation and/or low DNA concentration and no further attempts will be made to analyze that sample.

Deliverables

Samples will be analyzed and results provided to SEFSC within 90 days of submission.

The Contractor shall return the results of the axiom mini array as an Excel or comma separated (csv) file.

Data Rights

Data created, obtained and collected during the performance of this agreement is the property of NMFS. Data may not be released by any party without written consent from the NMFS POC. Data must be protected from unauthorized disclosure. Personnel must comply with all Federal, DOC, NOAA, and NMFS policies relating to IT and data security.

Non-Personal Service

Personnel performing services under this order will be controlled, directed and supervised at all times by management personnel of the contractor. Personnel will perform independent of and without the supervision of any Government official. Actions of contractor employees may not be interpreted or implemented in any manner that results in any employee creating or modifying Federal policy, obligating the appropriated funds of the U.S. Government, overseeing the work of Federal employees, providing direct personal services to any Federal employee or otherwise violating the prohibitions set forth in Parts 7.5 and 37.1 of the Federal Acquisition Regulations. The NMFS will perform the inspection and acceptance of the completed work.

Technical Point of Contact

Primary POC:

Dana Williams Phone: 305-582-5770 Email: Dana.Williams@noaa.gov

Secondary POC:

Matthew W. Johnson Phone: 305-361-4213 Email: Matthew.Johnson@noaa.gov

Period of Performance

Date of award through September 30, 2021 mailto:Dana.Williams@noaa.gov