NFFP7100_15_02973_NICHOLS_SOW.doc

STATEMENT OF NEED FOR SUPPLY OR SERVICE < $150K

NFFP7100-15-02973, CB32176

PURPOSE:

The NWFSC provides scientific and technical support to NMFS for the management, conservation, and wise use of the Northwest region's marine and anadromous resources. The NWFSC Conservation Biology and Environmental and Fisheries Science Divisions are interested the conservation of marine species and fisheries restoration and aquaculture, respectively. Obtaining next generation sequencing service is needed to fulfill our research objective to evaluate the genomic differences between groups of marine and freshwater species that are of conservation concern. The purpose of this requisition is to obtain high quality next generation sequencing data, as described in the technical specifications.

TECHNICAL SPECIFICATIONS:

The services required include 47 lanes of Illumina HiSeq2000 or HiSeq2500 DNA sequencing, and RNA sequencing library preparation services for 24 samples. The 47 lanes include sequencing of 39 individual libraries of genomic DNA in a single direction (single read or SR), at 100 nucleotides length, and 8 individual libraries of genomic DNA from both the forward and reverse directions (paired end or PE) with an error rate of <2% and producing at least 200 million reads per lane. Samples for sequencing will be prepared by Northwest Fisheries Science Center (NWFSC) staff and submitted one, two or three lanes at a time, with a maximum turnaround time of 2 months from sample submission and receipt at the sequencing facility, to delivery of data to the NWFSC. Sample submission and tracking must occur through an online queue such that NWFSC can monitor the submission, running, and completion of samples through this online queue in real time. The NWFSC will submit samples for sequencing at 5-10 nanomolar concentration with a minimum volume of 20 microliters. Once submitted, quality control of each sample is to be evaluated by the sequencing facility, and should include library quantification by quantitative polymerase chain reaction (qPCR) using the Illumina adapter primers (such as the Kapa qPCR protocol), as well as fragment size determination by the Advanced Analytical Fragment Analyzer, Agilent BioAnalyzer, or Agilent Tapestation. If samples are low quantity or poor quality as deemed by this QC at the sequencing facility, the sequencing facility shall notify the NWFSC to allow staff at NWFSC to decide whether or not the sequence the library in question. After sequencing is completed, all raw DNA sequence data will be provided to the NWFSC Molecular Genetics laboratory in a format that is downloadable from our user account. Raw sequencing data shall consist of flat text files containing genetic sequence and quality scores (fastq format), provided by the vendor for each lane of data immediately after sequencing. Library preparation for RNA sequencing libraries will include library construction using kits that enable individual sample barcodes and preparation for sequencing on the HiSeq2000 or HiSeq2500, and must include appropriate quality control, as above, for fragment analysis and sample quantification.

DELIVERABLE SCHEDULE:

Sequence data is to be delivered as FASTQ files of raw data immediately after sequencing each lane submitted by the NWFSC, and within two months of submission of the sample to the online queue. All samples must be completed no later than 31 July 2017.

TECHNICAL POINT OF CONTACT (TOC):

Krista Nichols

CLAUSES RELEVANT TO SERVICE CONTRACTS:

SECURITY

· Personnel performing work will work primarily off-site.

· Personnel performing work will not require access to NOAA facilities.

· Personnel performing work will not require a PIV or CAC.

· Personnel performing work will not require a NOAA email address.

· Personnel performing work will not require use of DOC owned IT Equipment (Laptop, Desktop Computer).

· Personnel performing work will not require Non-Government IT equipment to interconnect to a DOC network.

· Personnel performing work will not require access to non-public DOC/NOAA data.

DATA PRIVACY

Should work on this agreement require that personnel have access to Privacy information, personnel shall adhere to the Privacy Act, Title 5 of the U.S. Code, Section 552a and applicable agency rules and regulations.

Data created, obtained and collected during the performance of this agreement is the property of NMFS. Data may not be released by any party without written consent from the NMFS POC. Data must be protected from unauthorized disclosure. Personnel must comply with all Federal, DOC, NOAA, and NMFS policies relating to IT and data security.

NON-PERSONAL SERVICE

Personnel performing services under this order will be controlled, directed and supervised at all times by management personnel of the contractor. Personnel will perform independent of and without the supervision of any Government official. Actions of contractor employees may not be interpreted or implemented in any manner that results in any employee creating or modifying Federal policy, obligating the appropriated funds of the U.S. Government, overseeing the work of Federal employees, providing direct personal services to any Federal employee or otherwise violating the prohibitions set forth in Parts 7.5 and 37.1 of the Federal Acquisition Regulations. The NMFS will perform the inspection and acceptance of the completed work.

********************************Other Considerations********************************

GREEN PROCUREMENT PROGRAM (GPP):

FORMCHECKBOX

does not apply

FORMCHECKBOX

is compliant

GPP includes Recovered Materials Products, Biobased Products, Energy Efficient Products, Electronic Product Environmental Assessment Tool Products, Water Efficient Products, Alternative Fuels Vehicles/Alternative Fuels/Hybrids, Non-Ozone Depleting Products, Environmentally-Preferable Products and Services, and Waste Prevention and Recycling. (If this procurement should fall under the GPP, but is not compliant, a justification is required with concurrence from the Environmental Designee and OCIO designee, if applicable, and approved by the Head of the Contracting Office (HCO).

SERVICES: If this procurement is for a service (any part is Object Class 25xx), the following statement may need to be included (secretary will check this box if applicable, or remove this paragraph if not applicable):

FORMCHECKBOX

The C&A requirements of clause 73 do not apply, and a Security Accreditation Package is not required.

If this procurement involves a service (Object Class 25xx), your secretary will complete the Information Security in Acquisitions Checklist for you, but will need the following information:

Will this acquisition require services of contractor personnel?

FORMCHECKBOX

Yes FORMCHECKBOX No If yes,

· Will contractor require site access?

FORMCHECKBOX

Yes x FORMCHECKBOX No

· Will contractor require a permanent user account?

FORMCHECKBOX

Yes FORMCHECKBOX No

· Will contractor use their own IT system to interconnect to a DoC network?

FORMCHECKBOX

Yes FORMCHECKBOX No

· Will contractor have ability to access any non-public (privileged) data?

FORMCHECKBOX

Yes FORMCHECKBOX No