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STATEMENT OF WORK

TITLE: Collection of Mussels (Mytilus edulis) in Dorchestor Bay, Quincy, MA for candidate Standard Reference Material, SRM 1974d, Organics in Mussel Tissue (Mytilus edulis)

I. BACKGROUND INFORMATION

The Chemical Sciences Division (CSD), part of the National Institute of Standards and Technology (NIST) Material Measurement Laboratory, serves as the Nation's reference laboratory for chemical measurements and standards to enhance U.S. industry's productivity and competitiveness, assure equity in trade, and provide quality assurance for chemical measurements used for assessing and improving public health, safety, and the environment.

NIST maintains the Marine Environmental Specimen Bank (Marine ESB) at the Hollings Marine Laboratory (HML) in Charleston, South Carolina. The Marine ESB is an environmental specimen bank that cryogenically archives specimens collected as part of several monitoring and research programs supported primarily by other US government agencies, including, the National Oceanic and Atmospheric Administration (NOAA), US Geological Survey (USGS), and US Fish and Wildlife Service (USFWS).

In addition to maintaining the Marine ESB in Charleston, NIST also develops candidate Standard Reference Materials (SRMs) and Control Materials (CMs) to support accurate and compatible measurements for industry.

SRMs and CMs are valuable in industry settings to establish instrument calibrations to ensure quality assurance parameters, to validate the accuracy of specific measurements, and to support the discovery and development of new measurement procedures. The Reference Material Production Facility, housed within the Marine ESB, provides the equipment and capabilities to produce SRMs/CMs for certification. The supply of SRM 1974c, Organics in Mussel Tissue (Mytilus edulis) is expected to be depleted in a few years. Due to this being the 5th collection in a series of SRMs (SRM 1974, 1974a, 1974b, 1974c), it is important that the collection of mussels be in the same or in a pre-approved similar location as previous SRMs and using a standardized protocol.

II. SCOPE OF WORK

The contractor shall provide the following:

Apply and receive proper scientific collection permit for the collection of mussels in the Dorchester Bay, MA area.

Collect approximately 5,000-6,000 mussels (>8 cm in length) following the tide as it ebbs and flows over approximately 2-3 days. The quantity of mussels provided shall not be less than 5,000.

Rinse samples with seawater to remove mud and debris. After collection, the contractor shall place the mussels within the provided Teflon bags, seal them with a zip tie, and freeze until shipment.

Samples shall be shipped on dry ice or ice packs to the NIST-Charleston, Hollings Marine Laboratory, 331 Ft. Johnson Rd., Charleston, SC 29412 within 1 month after collection.

A summary report shall be provided that shall contain details on the field collection site (GPS location), date/time collected, time frozen, sample collectors, and any additional comments or deviations from the sampling protocol (ROA 839.02-04-009)

The place of performance is the coastal area of Dorchester Bay, Quincy, MA and the contractor’s laboratory facility.

All samples shall be delivered to NIST, Charleston, South Carolina. Normal duty hours are Monday through Friday, 8:30 a.m. to 5:00 p.m. with the exception of Federal holidays.

III. GOVERNMENT FURNISHED PROPERTY

One-hundred (100) 2mL thick Teflon bags, 12” x 12”, will be provided to the contractor for the storage of mussels after collection and rinsing. NIST will provide the bags once the award has been accepted.

IV. DELIVERABLES

Description Quantity Due Date

Common Mussels (Mytilus edulis) 5,000-6,000 60 days after award

Final Summary Report 1 75 days after award

V. ATTACHMENTS

Report of Analysis 839.02-04-009: Collection and Storage of Boston Harbor Mussels for SRM 1974c (see below)

VI. GOVERNMENT ACCEPTANCE

The Government technical point of contact will inspect and confirm that all minimum requirements in this document have been met for acceptance.

Requirements Acceptance Due Date

Delivery of 5,000-6,000 mussels as defined in the document.

Mussels are minimum 8 cm long Within 60 days after award.

Final Summary Report Within 75 days after award

Department of Commerce

NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY

CHEMICAL SCIENCE AND TECHNOLOGY LABORATORY

ANALYTICAL CHEMISTRY DIVISION

GAITHERSBURG, MARYLAND 20899

REPORT OF ANALYSIS

April 29, 2004

ROA Number: 839.02-04-009

Submitted To: Willie E. May, Chief

Authors: Barbara Porter and Rebecca Pugh

Title: Collection and Storage of Boston Harbor Mussels for SRM 1974c

Constituent: Mussels (Mytilus edulis) from Boston Harbor, Dorchester Bay, MA

Method: N/A

Copies To: B.J. Porter, R.S. Pugh, M.M. Schantz, S.A. Wise, and ROA Book

839.02-04-009

U.S. DEPARTMENT OF COMMERCE

NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY

CHEMICAL SCIENCE AND TECHNOLOGY LABORATORY

ANALYTICAL CHEMISTRY DIVISION

GAITHERSBURG, MARYLAND 20899

REPORT OF ANALYSIS

April 29, 2004

Collection and Storage of Boston Harbor Mussels for SRM 1974c

Submitted to:

Willie E. May

Chief

Analytical Chemistry Division

Chemical Science and Technology Laboratory

INTRODUCTION

The supply of Standard Reference Material (SRM 1974b), Organics in Mussel Tissue

(Mytilus edulis), is expected to be depleted in 2008. Due to the time required to prepare and certify a natural matrix SRM, the collection of mussels for the renewal material, SRM 1974c, was initiated in FY 2004. Approximately 10,000 mussels were collected by a private contractor following the National Oceanic and Atmospheric Administration

(NOAA) and National Institute of Standards and Technology (NIST) National

Biomonitoring Specimen Bank (NBSB) protocol, included in Appendix A. The details of the collection and storage information are covered in this report.

SAMPLING TEAM

The sampling crew consisted of five collectors and the project manager, Roger Fay, from

TDI-Brooks International, 1902 Pinon, College Station, TX 77845, phone 409-693-3446.

The sampling began on April 6, 2004 at 1700 h local time and was completed on April 8, 2004.

SAMPLING SITE

The mussels (Mytilus edulis) were collected from Boston Harbor, Dorchester Bay, MA.

The exact site for the collection was in Dorchester Bay at Marina Bay. This location is east of the large multicolored fuel storage tanks. The coordinates were measured at the site by GPS and were: WP14 – beginning collection (highest accessible tide) N 42

18.062’; W 071 01.993’, WP17 – ending collection (extend of low tide) N42 18.086’; W

071 01.953’. The collection sites (wp 14 and 17) are shown on the maps in Appendix A.

This is the same location as the previous collections of mussels for SRM 1974 (ROA

552-88-026), SRM 1974a (ROA 835-93-075) and SRM 1974b (ROA 839.02-00-016) had been collected. Additionally, Roger Fay sent a CD containing pictures of the location and the collecting process.

SAMPLE COLLECTION

Collection began on April 6 with the falling tide. Individual mussels were collected by hand from the expansive mussel beds in the intertidal zone. Collections were consolidated into wash baskets, rinsed with site water to remove mud, and dead specimens

(floaters or shells gaped open) were removed from the collection. Mussels were counted into Teflon bags (12” x 12”) and stored in ice chests. Ambient air temperatures were in the low 40 °F range at the time of collection.

Collection continued until a little after dark as the tide reversed and began to rise.

Samples were then transported back to New Bedford and stored (ice chest lids open) in a freezer warehouse on Fish Island. The samples remained frozen in the warehouse until

April 12.

The mussels were found to be considerably larger in the lower intertidal region than at the upper end where the collection began. This influenced the estimate of the number of individual mussels collected. At the beginning, 150 mussels constituted a full bag, and this estimate was used in determining the goal of 10,000 mussels. At the end of the collection period another count was made of bag contents, and it was found that 120 mussels filled the bag. Subsequently, another collection of approximately 1000 mussels was made on the morning of April 8 to ensure that there was a sufficient overage to provide a net of 10,000 mussels allowing for losses due to counting errors or mortality.

SAMPLE SHIPMENT

On April 12 the samples, contained in 12 ice chests, were loaded and trucked to Charleston, SC and delivered to the Hollings Marine Laboratory. The mussels remained frozen and were delivered on April 13.

SAMPLE STORAGE

The mussels were unloaded at the NBSB in Charleston, SC on April 13, 2004 and put into the “L” liquid nitrogen vapor phase freezer. The mussels will remain there until required for shucking. This collection is contained in 84 individual bags. Each bag contains at least 100 mussels, with some bags containing 120 and some containing 150.

Information for this ROA was obtained from phone conversations with Roger Fay and

Rebecca Pugh and from email messages from Roger Fay.

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Analyzed by:

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Barbara J. Porter

Biologist

Rebecca S. Pugh/

Research Biologist

Michele M. Schantz, Ph.D.

Research Chemist

APPENDIX A

Maps of Sampling Location

I