Attachment _ HHS-NIH-NIDA-NIA-CSS-20-006607.pdf

Combined Synopsis/Solicitation: HHS‐NIH‐NIDA‐NIA‐CSS‐20‐006607

Title: Custom Scientific Computer System

Minimum Specifications

1. Custom Configured High‐End Workstation for Big Image Data Processing and Analysis

System. Quantity: 1 Each.

2. Phanteks Enthoo 719 (PH‐ES719LTG_DAG01) High Performance Full Tower Case –

Tempered Glass, Aluminum Panels, Dual System/PSU Support, Massive Storage, Fan Hub, Digital‐RGB Lighting, Anthracite Grey. Quantity: 1 Each.

3. Phanteks ‐ Stackable 3.5" HDD Bracket Duo Pack Cases PH‐HDDKT_03. Quantity: 7 Each.

4. Noctua Fan NF‐A14 PWM 140x140x25mm 4Pin SSO2 Bearing A‐Series Blade Geometry

Retail 3 x Front Intake, 3 x Top Exhaust, 1 x Rear Exhaust. Quantity: 7 Each.

5. EVGA SuperNOVA 1600 T2 Power Supply, ATX12V/EPS12V ‐ 120 V AC, 230 V AC Input

Voltage ‐ 3.3 V DC, 5 V DC, 12 V DC, 5 V DC, ‐12 V DC Output Voltage ‐ Modular ‐ 96% Efficiency ‐ 1600 W. 10 Year Warranty. Quantity: 1 Each.

6. Supermicro H11DSI‐NT Server Motherboard ‐ AMD Chipset ‐ Socket SP3 ‐ 2 TB DDR4

SDRAM, Maximum RAM ‐ DIMM, RDIMM ‐ 16 x Memory Slots ‐ 2 x USB 3.0 Port ‐ 10 x SATA Interfaces, Dual 10GBase‐T LAN Ports , 2 PCI‐E 3.0 x16, 3 PCI‐E 3.0 x8, 2 USB 3.0 ports, 4 USB 2.0 ports. Quantity: 1 Each.

7. Supermicro TPM 2.0 module with Infineon 9665, RoHS/REACH,PBF. Quantity: 1 Each.

8. AMD EPYC (2nd Gen) 7742 Tetrahexaconta‐core (64 Core) 2.25 GHz Processor ‐ Retail

Pack, 256 MB Cache ‐ 3.40 GHz Overclocking Speed ‐ 7 nm ‐ Socket SP3 ‐ 225 W ‐ 128 Threads, 3400MHZ. Quantity: 2 Each.

9. Noctua NH‐U12S TR4‐SP3, Premium‐Grade CPU Cooler for AMD sTRX4/TR4/SP3.

Quantity: 2 Each.

10. Noctua NF‐F12 PWM, Premium Quiet Fan, 4‐Pin (120mm, Brown), Push Pull. Quantity: 2

Each.

11. DDR4 LRDIMM‐3DS STD 128GB 8RX4 2666, Total memory Installed 2048GB (2TB).

Quantity: 16 Each.

12. Intel DC P4511 2 TB Solid State Drive ‐ M.2 22110 Internal ‐ PCI Express (PCI Express 3.1 x4), 1 Pack 110MM PCIE 3.1 X4 3D2 TLC, Primary/Boot. Quantity: 1 Each.

13. NVIDIA VCQRTX8000‐PB NVIDIA Quadro RTX 8000 Graphic Card ‐ 48 GB, Primary Card.

Quantity: 1 Each.

14. NVIDIA Quadro GV100 32GB Volta Workstation Graphics Card. Detailed Specifications:

CUDA Cores 5120, Tensor Cores 640, Peak Double Precision FP64 Performance 7.9 TFLOPS, Peak Single Precision FP32 Performance 14.8 TFLOPS, Peak Half Precision FP16 Performance 29.6 TFLOPS, Peak Integer Operation (INT8) Performance 59.3 TFLOPS, Deep Learning TFLOPS, 118.5 TFLOPS, GPU Memory 32 GB HBM2, Memory Interface 4096‐bit, Memory Bandwidth 870 GB/s, System Interface PCI Express 3.0 x16, Display Connectors DP 1.4 (4) + DVI‐D DL (1) + Stereo. Compute Card. Quantity: 1 Each.

15. Intel Tri‐Mode PCIe/SAS/SATA Full‐Featured RAID Adapter, 8 Internal Ports, 12Gb/s SAS, Serial, ATA/600 ‐ PCI Express 3.0 x8 ‐ Plug‐in Card ‐ RAID Supported ‐ 0, 1, 10, 5, 50, 6, 60, JBOD RAID Level ‐ 8 Total SAS Port(s) ‐ 8 SAS Port(s) Internal ‐ 0 SAS Port(s) External ‐ Linux, PC – 4 GB. Quantity: 1 Each.

16. Intel® RAID Maintenance Free Backup AXXRMFBU7. Maintenance Free Backup Unit designed for the RMSP3AD160F and RMSP3CD080F Intel(r) Integrated RAID Modules, and RSP3DD080F, RSP3MD088F, and RSP3TD160F Intel(r) RAID Adapters. Quantity: 1 Each.

17. Internal HD Mini SAS (SFF‐8643) to 4 SATA Forward Breakout Cable 3.3 Feet / 1m.

18. Nimbus Data 32TB 3.5" Enterprise class SATA SSD. Durability: 1 DWPD (5 Year), 15W

TDP. Quantity: 4 Each.

19. SETUP RAID 0 (striped) 128TB RAW internal SSD storage. Quantity: 1 Each.

20. Nimbus Data 32TB 3.5" Enterprise class SATA SSD. Durability: 1 DWPD (5 Year), 15W

TDP, , Archival internal SSD storage. Quantity: 1 Each.

21. MiniSAS to 4x SATA 51/51cm Cable (CBL‐0097L‐03), Quantity: 1 Each.

22. Microsoft Windows 10 Pro 64‐bit for Workstations ‐ License ‐ 1 License – OEM.

Quantity: 1 Each.

23. Dell UltraSharp UP3218K 31.5" 8K LED LCD Monitor ‐ 16:9 ‐ Black ‐ 7680 x 4320 ‐ 1.07

Billion Colors ‐ 400 Nit ‐ 6 ms GTG – DisplayPort. Quantity: 2 Each.

24. Logitech Illuminated Ultrathin Keyboard K740 with Laser‐etched Backlit Keyboard and

Soft‐touch. Palm Rest. Quantity: 1 Each.

25. Logitech Corded Mouse M500 ‐ Laser ‐ USB ‐ Black, Gray. Quantity: 1 Each.

26. Tripp Lite USB 3.0 Hub SuperSpeed 10‐Port USB‐A Compact 20kV ESD Industrial.

27. Install all purchased components.

28. System must be assembled with operating system installed, loaded and tested.

29. Thirty‐Six (36) Month Parts, Replacement, & Repair Warranty.

30. Individual items in system may have varying manufacturer warranties which should be for a minimum of three (3) years.

31. Site Delivery, Set‐up, and Testing.

32. Anticipated Delivery Period six (6) to eight (8) weeks after receipt of order.

Skip Navigation

| | Bookmark & Share Home > Research Facilities > NINDS Flow Cytometry Core Facility

Contact Us

Staff Contact List Faculty Search for Staff

For Staff

Research Support Facilities Seminars & Special Interest Groups Other Resources and Information

Center for Molecular Modeling

BioWulf at NIH

NIH Intramural Sequencing Center

NIH Intramural Research Program

NINDS Flow Cytometry Core Facility

Facility Staff:

Dragan Maric, Ph.D., Facility Manager 10 Center Drive, Building 10, MSC 1444 Room 5N-240A (Office) / Room 5S-241 (Lab) Bethesda, MD 20892 Phone: (301) 402-1406 E-mail: maricd@ninds.nih.gov

Andrea Sedlock, M.S., Biologist 10 Center Drive, Building 10, MSC 1444 Room 5S-238 Bethesda, MD 20892 Phone: (301) 402-6934 E-mail: andreasedlock@mail.nih.gov

Scope of Facility:

The primary mission of the Facility is to provide intellectual, technical, collaborative and new application research and development support to NINDS and other intramural investigators in both basic and clinical research programs requiring the use of high-throughput conventional flow cytometry (CFC), imaging flow cytometry (IFC), preparative fluorescence-activated cell sorting (FACS) and multi-spectral in situ cytometric imaging (ISCI). The Facility currently provides routine use of CFC, IFC, FACS and ISCI applications in assaying a multitude of biological properties at the cellular, subcellular and molecular levels, as specified by the investigator. Critical to these activities is the research and development of a wide variety of novel, customized and standardized multiplex biomarker screening protocols using unique combinations of fluorescent reporters of interest, including those identifying different stages of cell proliferation and differentiation, as well as different types of cell death (i.e., apoptotic, necrotic, cytotoxic, autophagy), in addition to those assaying functional gene expression and cell signaling. The aim of these protocols is to maximize the simultaneous detection and quantification of multiple biological properties per cell and its subcellular compartments (i.e., plasma membrane, cytoplasm, nucleus, mitochondria, lysosomes, synaptosomes, microvesicles, exosomes), utilizing an array of appropriate cell surface, cytoplasmic, nuclear, protein and gene expression biomarkers, as well as select physiological indicator dyes (potentiometric, calcium, pH, live/dead), linked to fluorescent endpoints. Depending on the investigator’s requirements, the Facility provides high-throughput screening of cells and assaying of their specific biological properties, in addition to preparative sorting of purified cell populations and/or subpopulations of interest, based on one or more specific cellular/subcellular parameters, as specified by the investigator. Additional responsibilities of the Facility personnel include regular maintenance and technological upgrades of all instruments that are managed by the Facility, as well as daily instrument alignment and calibration, setting up sample quality controls and standardization, and enabling seamless data acquisition and analysis, presentation and interpretation of the resulting findings to the investigator, and, if requested by the investigator, manuscript preparation and critical review.

Click the below links for more information:

◾ Facility Equipment ◾ How to Use the Facility ◾ Selected Publications Using the Facility searchContact Us | Follow Us search

Home Faculty Research Areas Patient & Clinical Programs Research Facilities Jobs & Training Events

Skip Navigation

| | Bookmark & Share Home > Research Facilities > NINDS Flow Cytometry Core Facility > Facility Equipment

Center for Molecular Modeling

BioWulf at NIH

NIH Intramural Sequencing Center

NIH Intramural Research Program

Services - conventional flow cytometry (CFC) and preparative cell sorting (FACS)

Excitation - 7 air-cooled lasers (355, 405, 488, 532, 561, 592, 640 nm)

Emission

Dyes

- up to 30 (peak emissions ranging from near-UV to near-IR spectrum)

- 20x20 digital compensation matrix for spectrally overlapping dyes

- DAPI, FITC, PE, PE-TR, PE-Cy5, PE-Cy7, Pacific Blue, Pacific Orange

- Alexa 350, 405, 488, 546, 594, 647, 700, 750 or their DyLight equivalents

- Hoechst 33342 (blue/red emissions), PI, GFP, YFP, Venus, tdTomato, mCherry

- Qdot 525, 545, 565, 585, 605, 625, 655, 800

Acquisition - up to 1x105 cells/sec, up to 1x109 cells per acquisition

Sorting - simultaneous 6-way sorting (enrich, pure, single cell)

- up to 5x104 sorted cells/sec

- sort collection (1.5-50 ml tubes, 6-96 well plates, 1x3” microscope slides)

Bioassays - cell cycle and mitosis (DNA content, BrdU incorporation)

- cell death (apoptosis, necrosis, cytotoxicity)

- cell phenotyping (any combination of 20 fluorescent biomarkers)

- cell signaling (potentiometric, calcium flux, protein phosphorylation)

- gene expression (GFP+, YFP+, RFP+ transfected, transduced, transgenic cells)

- preparative cell sorting on any combination of up to 20 fluorescent biomarkers

Facility Equipment

The Facility provides seamless utilization of the following equipment: MoFlo Astrios cell sorter (Beckman Coulter), ImageStreamX flow cytometer (Amnis Corporation) and AxioImager.Z2 10-channel wide-field fluorescence microscope with motorized scanning stage (Carl Zeiss). The optical configurations and services currently provided with each instrument are listed below:

MoFlo Astrios

ImageStreamX Flow Cytometer searchContact Us | Follow Us search

Home Faculty Research Areas Patient & Clinical Programs Research Facilities Jobs & Training Events

Services - imaging flow cytometry (IFC)

Excitation - up to 7 air-cooled lasers (3 currently available: 405, 488, 658 nm)

Emission - up to 10 (peak emissions ranging from near-UV to near-IR spectrum)

- 10x10 digital compensation matrix for spectrally overlapping dyes

Dyes - DAPI, FITC, PE, PE-TR, PE-Cy5, PE-Cy7, Pacific Blue, Pacific Orange

- Alexa 405, 488, 633, 647 or their DyLight equivalents, APC, APC-Cy7

- GFP, YFP, RFP, Qdot 525, 545, 565, 585, 605, 625, 655, 800

Acquisition - up to 2x103 imaged cells/sec, up to 1x105 imaged cells per acquisition

Bioassays - cell cycle and mitosis (DNA content, BrdU incorporation, stages of cell mitosis)

- cell death (apoptosis, necrosis, cytotoxicity, autophagy)

- cell phenotyping (any combination of 10 fluorescent biomarkers)

- cell signaling (intracellular protein trafficking, phosphorylation, enzyme activity)

- cell-cell interactions (intercellular protein trafficking, immune synapse, etc.)

- translocation (membrane to cytoplasm or vice versa, cytoplasm to nucleus, etc.)

- co-localization (ligand-receptor, two or more receptor subunits, enzyme-substrate)

- gene expression (GFP+, YFP+, RFP+ transfected, transduced, transgenic cells)

- internalization (pinocytosis, phagocytosis)

Services - multispectral in situ cytometric imaging (ISCI) Excitation

Excitation - mercury arc lamp (light filtered using 10 spectrally-compatible exciter filters)

Emission - up to 10 (peak emissions ranging from near-UV to near-IR spectrum)

Dyes - Alexa 350, 405, 430, 488, 546, 594, 647, 700, 750, IRDye800CW, PerCP

Acquisition - scan areas up to 1 cm2 in brightfield and up to 10 fluorescence channels

Bioassays - cell cycle and mitosis (DNA content, BrdU incorporation, stages of cell mitosis)

- cell death (apoptosis, necrosis, cytotoxicity, autophagy, cell morphology)

- cell phenotyping (any combination of 10 fluorescent biomarkers)

- cell signaling (intracellular protein trafficking, phosphorylation, enzyme activity)

- cell-cell interactions (intercellular protein trafficking, immune synapse, etc.)

- gene expression (GFP+, YFP+, RFP+ transfected, transduced, transgenic cells)

- systems biology (whole embryo and brain scans, phenotyping tissue cytoarchitecture)

AxioImager.Z2 Scanning Widefield Fluorescence Microscope