FWC SS 22.23-22 PUR7776 FINAL SP.pdf

PUR 7776 (Revised 07/2022) Page 1 of 2 Incorporated by Reference: Rule 60A-1.045, F.A.C.

DESCRIPTION OF INTENDED

SINGLE SOURCE PURCHASE

(PUR 7776)

AGENCY: Florida Fish and Wildlife Conservation Commission

TITLE: Yokogawa Fluid Imaging Technologies Bench Top FlowCam Short description of the commodity or service desired: A benchtop imaging flow cytometer that captures high-resolution, digital images of particles and organisms ranging in size from 3mm up to 1mm that are suspended in a liquid medium such as seawater or culture media.

CONTACT

Name: Sarah Pegram Address: 1875 Orange Ave E, Tallahassee FL 32311 Telephone: 850-410-0471 Email: sarah.pegram@myfwc.com

Internal tracking number, if any: FWC SS 22/23-22

Date posted: 6.6.2023 Last day for receipt of information: 6.27.2023

This description of commodities or contractual services intended for purchase from a single source is posted in accordance with Sections 120.57(3) and 287.057(3), Florida Statutes (F.S.), and will remain posted for a period of at least 15 business days.

Commodity or Contractual Service Required (commodity or United National Standard Products and Services Code (UNSPSC), manufacturer, model, and description, as appropriate):

Yokogawa Fluid Imaging Technologies Bench Top FlowCam 8400 81101706: Laboratory equipment maintenance 81141504: Equipment test calibration or repair 41116500: Instrument parts and accessories 41100000: Laboratory and scientific equipment 41104000: Sampling equipment 41116148: Flow cytometry test kits or supplies 41116152: Flow cytometry quality controls and calibrators and standards

Quantity or Term (as appropriate):

Up to 3 years - upon issuance of purchase order

Requestor (division, bureau, office, individual, as appropriate):

Kate Hubbard, Fish and Wildlife Research Institute

Performance and/or Design Requirements (e.g. intended use, function or application, compatibility, requirements; reference to policy, rule, statute or other act of the Legislature, etc., as appropriate):

PUR 7776 (Revised 07/2022) Page 2 of 2 Incorporated by Reference: Rule 60A-1.045, F.A.C.

The Bench Top FlowCam 8400 is a benchtop imaging flow cytometer that captures high-resolution, digital images of particles and organisms ranging in size from 3mm up to 1mm that are suspended in a liquid medium such as seawater or culture media. This instrument is used for screening harmful algal bloom monitoring and event response samples and can easily switch between live and Lugol's-fixed samples.

Intended Single Source:

Yokogawa Fluid Imaging Technologies, Inc.

Estimated Dollar Amount:

$122,000.00

Justification for single source acquisition (what is necessary and unique about the product, service or source; steps taken to confirm unavailability of competition, as appropriate):

Yokogawa Fluid Imaging Technologies, Inc. is the sole manufacturer and sole distributor of the FlowCam in the United States and Canada. The FWRI HAB team has been implementing the FlowCam to help screen routine and event response samples, including hundreds of archived samples, since 2021 and has established SOPs for Florida HABs and our most commonly used fixative, Lugol's solution (a standard iodine-based reagent used for safely fixing cells at the time of collection). Another instrument is requested to help increase sample throughput. In addition to providing consistency of analysis and data to the HAB group, this is the only bench top cytometer that has the Automated Liquid Handling (ALH) for FlowCam™ which seamlessly integrates with FlowCam 8000 Series instruments to enable automated sample preparation and analysis with state-of-the-art robotic handling via a micro-syringe pump and fluidics system that can be reset or replaced by the user for rapid use with different buffers, solvents, or fixatives.

This includes Lugol's fixative, which cause staining over time and thus requires regular user replacement of the fluidics tubing to maintain sample integrity.

Approved By (names & titles, as appropriate, e.g., requestor, requestor management, information systems, budget, purchasing):

Kate Hubbard, Research Scientist, Requestor Tyler Herret, Operations & Managerment Consultant 1, Budget Justin Hooper, Network Systems Administrator, OIT Approval Caroline Gorga, Section Leader Leanne J. Flewelling, Deputy Director Rachel Korade - Assistant Purchasing Director Renee Gregory - Purchasing Director

Prospective vendors are requested to provide information regarding their ability to supply the commodities or contractual services described. If it is determined in writing by the agency, after reviewing any information received from prospective vendors, that the commodities or contractual services are available only from a single source, the agency shall provide notice of its intended decision to enter a single-source purchase contract in the manner specified in Rule 60A- 1.045, Florida Administrative Code, and section 120.57(3), F.S.