Supplement_SSJ_FBO.pdf

Supplement to Single Source Justification, Proposed Contractor is: Dr. Osam Mazda

Detailed itemized expenditures for the budget proposed for the project: “Establishment of clinically feasible direct reprogramming procedures leading to a cell‐based therapy for bone injury”.

Overall budget:

Material and supplies $ 84,460

Personnel cost $ 19,540

Travel cost $ 8,500

Indirect Cost $ 12,500

Total: $125,000

This Overall budget is itemized as follows:

Materials and supplies $84,460

Cells, culture medium, serum = $9,400

Cells (Fibroblasts, Osteoblasts, Keratinocytes, MSCs, Flat‐GP packaging cells) …$3,800

DMEM medium, Trypsin, FBS, Albumin, Pyruvate, Nonessential amino acids, HEPES, Insulin…$1,400 antibiotics, Ficoll, cell count chambers, cell freezing buffer, CO2…$2,100 competent bacteria, bacterial media, agar plates…$2,100

Chemicals, reagents, enzymes = $10,100

Buffer, ethanol, ascorbic acid, beta‐glycerophosphate, dexamathasone, OCT compound…$1,500

Staining dye (Mitotracker, , von Kossa, Alizarin S, DAPI, hematoxylin, Eosin, Hoecht)…$1,300

DNA ligases and polymerases, Reverse transcriptases, Restriction endonucleases, ALP, RNase…$1,500

Plasmid DNA, cDNA clones, collagen, acrylamide, agarose, gel, DEPC water…$2,900

Phenol, Chloroform, liposomes, paraformaldehyde, matrigel, recombinant cytokines, anethetics…$2,900

Antibodies, kits = $9,200

Anti‐osteocalcin, anti‐osteopontin, anti‐ALP, anti‐mouse Ig and other antibodies, magnetic beads…$3,200

Retrovirus quantification kits, Tetrazolium salt assay kits, TaqMan kits…$3,200

RNA extraction kits, RT kits, real time PCR reagents kits, ELISA kits, apoptosis detection kits…$2,800

Synthetic DNA, synthetic RNA = $5,200

Primer oligodeoxyribonuceotides, Probe oligodeoxyribonucleotides…$4,200

Guide RNA…$1,000

DNA chips, columns = $7,100

DNA chip array, fluorescence probes…$3,800

DNA purification column, distilled water columns…$3,300

Animals and maintenance = $4,600

Mice (C57/BL6, SCID, NOD‐SCID, SCID gamma KO)…$2,500

Feed for mice, usage fee for animal facility…$2,100

Plastic and glass wares = $12,600

Centrifuge tubes, sampling tubes, microfugation tubes, tube holders, tube storage box…$2,000 culture dishes (35, 60, 100 mm), culture plates (6, 12, 24, 48, 96 wells)…$2,200 culture bottles (25 and 75 cm2), bottles (100, 500, 1000 mL), bottle filters, sealer…$2,200 syringe (1, 5, 10 mL), syringe filters, pipettes (1, 5, 10, 20 and 50 mL) …$1,300

Pipetmans (20, 100, 200, and 100 uL), Pipetaid, cryosection folders …$1,800

Pipetman tips (20, 100, 200, and 100 uL), Pipetman tips with filter (20, 100, 200, and 100 uL)…$1,800 cell scraper, microtiter plates, PCR tubes, gloves, masks, microscope lens …$1,300

Metal wares = $3,500

Surgical knifes, forceps, drills, calipers, syringe needles…$1,000 cabinet for experimental reagents …$1,500 centrifuge roters and brackets…$1,000

Printing expenses, stationery = $3,700

Printers, ink cartridges, printer rollers, printer belts, printer papers…$2,100 paper towels, pens, folders, tapes, pastes, scissors, clips, labeling tapes, fro experiments…$1,600

Communications expenses, PCs, devices and softwares = $9,800

WiFi, LAN usage fee…$1,400

Softwares for experimental devices such as DNA microarray …$2,400 softwares for fluorescence microscopic observation and quantitative analyses…$2,400

PCs, printers, scanners, cameras and storage media for experiments…$3,600

Others = $9,260

Refrigerators, batteries, bulbs, soap, plastic wrap, aluminum wrap, water pumps …$4,800 books, journals and reprints, submission fee for scientific journals …$2,460 usage fee for experimental facility and devices …$2,000

Personnel $19,540

Technicians (A full‐time technician will devote 25% of his/her time to this project to prepare samples for analysis and record results). Calculation: $23/hour, 8 hours/day, 5 days/week, 38 weeks/year equals $34,960, and $34,960 x 25%

= $8,740.

Postdoc (A full‐time postdoc will devote 36% of his/her time to this project to perform analysis). Calculation: 9 months salary of the postdoc will be $30,000, and $30,000 x 36% = $10,800. Market Research shows that 12 months salary for postdoc in University of Tokyo is Y4,800,000 ($39,199.07, FY17 Ex. Rate: $1=Y122.4519) https://www.glassdoor.com/Salaries/tokyo‐postdoctoral‐salary‐SRCH_IL.0,5_IM1071_KO6,18.htm. Based on this amount, 9 months salary will be Y3,600,000 ($29,399.30, $1=Y122.4519). Proposed 9 months amount of $30,000 and market research amount of $29,399.30 is comparable. Therefore, 9 months salary of the postdoc amount of $10,800 is considered fair and reasonable.

Travel $8,500

Domestic (to attend scientific conferences): Calculations: $500 for each trip from Osaka to Tokyo ($250 for traffic and another $250 for accommodation of 2 nights). 1 person will travel 3 times trips a year, and another 1 person will travel 2 times trips a year. Therefore, in total, $500 x 5 times trips = $2,500

International (to attend scientific conferences): Calculations: $3,000 for each trip from Japan to US ($2,500 for traffic and $500 for accommodation of 4 nights). 2 persons will travel for 1 time a year. In total, $3,000 x 2 times trips = $6,000

Indirect Cost $12,500

Buildings and equipment

1. Maintenance and rental costs for laboratory and office …$2,755

2. Electricity, gas, water, sewer, waste …$2,610

3. PCs, printers, scanners, storage media, books and stationery for company's routine operation …$2,440

4. Desks, chairs, storage lockers, cleaning supplies …$1,305

Administrative

1. Phone, fax and internet bills, postage, and delivery …$980

2. Fringe benefit, and clerical assistance labor costs …$1,560

3. Meeting costs, web page production …$850

Total Expenditures $125,000