ESBD_404451_1743083901286_4_Attachment F 304T-25-175A1 Lab Supplies.pdf

Texas Comptroller of Public Accounts

Statewide Procurement Division

IFB No. 304T-25-175A1

Proprietary Chemical Laboratory Equipment and Supplies

Attachment F

Requirements and Specifications

Solicitation No. 304T-25-175A1 Attachment F – Requirements and Specifications

Table of Contents

Definitions 175-14 - Chromatography Supplies: Developing Tanks, Columns, Packing, Septums, TLC Plates ... 5

Proprietary Line 1: Cap, Screw, AVCS, 9 mm

175-23 - DNA or RNA Acids Nucleic, etc.: Including Extraction, Purification, Quantification and Sequencing Equipment (lines 2-46)

Proprietary Line 2: EZ 1&2 DNA Investigator Kit, 48 Preps

Proprietary Line 3: EZ1 Advanced XL DNA Investigator Card

Proprietary Line 4: EZ1 Advanced XL DNA Investigator Flip-Cap Card

Proprietary Line 5: EZ1 Advanced XL DNA Investigator Flip Cap Tube Rack

Proprietary Line 6: EZ1 DNA Investigator Card

Proprietary Line 7: GlobalFiler Express PCR Amplification Kit, 200 Reactions

Proprietary Line 8: GlobalFiler Express PCR Amplification Kit, 1000 Reactions

Proprietary Line 9: Identifiler Direct PCR Amplification Kit, 200 Reactions

Proprietary Line 10: Investigator 24plex GO! Kit, 200 Reactions

Proprietary Line 11: Investigator 24plex GO! Kit, 1000 Reactions

Proprietary Line 12: Investigator 24plex QS Kit, 100 Reactions

Proprietary Line 13: Investigator 24plex QS Kit, 400 Reactions

Proprietary Line 14: Investigator Lyse&Spin Basket Kit, 250 Preps

Proprietary Line 15: Investigator STR Go! Lysis Buffer, 200 Swab Samples

Proprietary Line 16: Investigator STR Go! Punch Buffer, 200 Punch Samples

Proprietary Line 17: Investigator STR Go! Punch Buffer, 1000 Punch Samples

Proprietary Line 18: QIAamp DNA Mini Kit, 50 Preps

Proprietary Line 19: QIAamp DNA Mini Kit, 250 Preps

Proprietary Line 20: Qiagen 8-Rod Covers

Proprietary Line 21: Qiagen BT6 Matrix Standard

Proprietary Line 22: Qiagen Buffer AL

Proprietary Line 23: Qiagen Buffer ATL

Proprietary Line 24: Qiagen Buffer G2

Proprietary Line 25: Qiagen Buffer MTL

Proprietary Line 26: Qiagen Collection Tubes, 2 mL

Proprietary Line 27: Qiagen Control DNA 9948

Proprietary Line 28: Qiagen Elution Tubes, 1.5 mL

Proprietary Line 29: Qiagen Filter-Tips, 200 µl

Proprietary Line 30: Qiagen Filter-Tips, 1500 µl

Proprietary Line 31: Qiagen Filter-Tips, Conductive, 50 µl

Proprietary Line 32: Qiagen Filter-Tips, Conductive, 200 µl

Proprietary Line 33: Qiagen Filter-Tips, Wide Bore, 1000 µl

Proprietary Line 34: Qiagen Proteinase K, 10 mL

Proprietary Line 35: Qiagen Reagent Bottle, 30 mL

Proprietary Line 36: Qiagen Rotor Adapter Holder

Proprietary Line 37: Qiagen Rotor Adapter, 10x24

Proprietary Line 38: Qiagen Sample Prep Cartridges, 8-well

Proprietary Line 39: Qiagen S-Blocks, 2.2 mL

Proprietary Line 40: Qiagen Shaker Rack Plugs

Proprietary Line 41: Qiagen Tape Pads

Proprietary Line 42: Qiagen Tubes, Graduated, Flat Base, 5 mL

Proprietary Line 43: QIAsymphony DNA Investigator Kit, 192 Preps

Proprietary Line 44: Tips, LiHa, Disposable, Filtered, Hanging, 50 µL

Proprietary Line 45: Tips, LiHa, Disposable, Filtered, Hanging, 200 µL

Proprietary Line 46: Yfiler Plus PCR Amplification Kit, 500 Reactions

175-67 - Pipetters, Pullers and Dispensers Proprietary Line 47: Multi-Pipette, Manual, Eight-Channel

Proprietary Line 48: Pipet, Electronic Repeater, Positive Displacement

Proprietary Line 49: Pipet, Manual, Single-Channel, 2 µL

Proprietary Line 50: Pipet, Manual, Single-Channel, 10 µL

Proprietary Line 51: Pipet, Manual, Single-Channel, 20 µL

Proprietary Line 52: Pipet, Manual, Single-Channel, 100 µL

Proprietary Line 53: Pipet, Manual, Single-Channel, 200 µL

Proprietary Line 54: Pipet, Manual, Single-Channel, 1000 µL

175-68 - Plastic Ware, All Types Proprietary Line 55: Trough, Disposable, 100mL, Transparent

Definitions

TERM DEFINITION

Aliquot A portion of a larger whole, especially a sample taken for chemical analysis or other treatment

Assay Analysis (as of an ore or drug) to determine the presence, absence, or quantity of one or more components

Chromatography A process in which a chemical mixture carried by a liquid or gas is separated into components as a result of differential distribution of the solutes as they flow around or over a stationary liquid or solid phase

DNA

Any of various nucleic acids that are usually the molecular basis of heredity, are constructed of a double helix held together by hydrogen bonds between purine and pyrimidine bases which project inward from two chains containing alternate links of deoxyribose and phosphate, and that in eukaryotes are localized chiefly in cell nuclei

Polymerase Chain Reaction (PCR) Laboratory technique used to amplify DNA sequences

Septum/Septa An engineered membrane that permits transfer of a substance without contact with air, usually using a syringe with needle

Sterile Free from living organisms, especially pathogenic microorganisms, and

DNA

175-14 - Chromatography Supplies: Developing Tanks, Columns, Packing, Septums, TLC Plates

Proprietary Line 1: Cap, Screw, AVCS, 9 mm Proprietary Brand/Product No.: Thermo Scientific # 6ACS9RT1Y Note: For use with Agilent Auto-Samplers

1. Cap Size: 9 mm

2. Cap Closure Type: Advanced Vial Closure System (AVCS)

3. Septa type: Ivory PTFE/red rubber

4. Septa Thickness: 1.0 mm

5. Closure Color: Yellow

6. Closure Material: Polypropylene

7. Center Hole Size: 6 mm

8. Packaging: 100 per pack

9. UOM: Pack

175-23 - DNA or RNA Acids Nucleic, etc.: Including Extraction, Purification, Quantification and Sequencing Equipment (lines 2-46)

Proprietary Line 2: EZ 1&2 DNA Investigator Kit, 48 Preps Proprietary Brand/Product No.: Qiagen, Inc. # 952034 Note: For use with Qiagen EZ2 Connect Fx, EZ1 Advanced XL, EZ1 Advanced and BioRobot® EZ1instruments.

1. Contents:

a. Reagent Cartridge, DNA Investigator: 48

b. Disposable Tip Holders: 50

c. Disposable Filter-Tips: 50

d. Sample Tubes (2 mL): 50

e. Elution Tubes (1.5 mL): 50

f. Buffer G2: 2 x 13 mL

g. Proteinase K: 1 x 1.2 mL

h. Carrier RNA: 1 x 310 μg

i. Q-Card: 1

2. Shall be able to be stored dry at room temperature (15-25°C).

3. Number of Preps: 48

4. UOM: Kit

Proprietary Line 3: EZ1 Advanced XL DNA Investigator Card Proprietary Brand/Product No.: Qiagen, Inc. # 9019933 For use with BioRobot EZ1 DNA Investigator protocols

1. Contains protocols for purification of DNA from forensic and human identity samples

2. For use in combination with EZ1 Advanced, EZ1 Advanced XL and BioRobot EZ1 protocols.

3. UOM: Each

Proprietary Line 4: EZ1 Advanced XL DNA Investigator Flip-Cap Card Proprietary Brand/Product No.: Qiagen, Inc. # 9022763 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Dimensions: 85L x 54W x 3H mm

2. Stainless Steel housing

3. External ROM memory card

4. Bar coded

5. Preprogramed with software script used for setup of protocols using the Qiagen EZ1 DNA

Investigator Kit on the flip-cap rack on the EZ1 Advanced XL.

6. Preprogrammed with protocols for purification of DNA from forensic and human-identity samples using the EZ1 DNA Investigator Kit on the EZ1 Advanced XL with the flip-cap rack.

7. UOM: Each

Proprietary Line 5: EZ1 Advanced XL DNA Investigator Flip Cap Tube Rack Proprietary Brand/Product No.: Qiagen, Inc. # 9022818 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Used in conjunction with EZ1 Advanced XL DNA Investigator Flip-Cap Card on the EZ1 Advanced XL.

2. Tube rack must fit Qiagen 2 mL sample tubes and Qiagen 1.5 mL elution tubes

3. UOM: Each

Proprietary Line 6: EZ1 DNA Investigator Card Proprietary Brand/Product No.: Qiagen, Inc. # 9016387 For use with BioRobot EZ1 DNA Investigator protocols

1. Contains protocols for purification of DNA from forensic and human identity samples

2. For use in combination with EZ1 DNA Investigator Kits.

3. UOM: Each

Proprietary Line 7: GlobalFiler Express PCR Amplification Kit, 200 Reactions Proprietary Brand/Product No.: Life Technologies Corporation # 4476609

1. Each kit shall contain:

a. GlobalFiler Express Master Mix, 1 bottle

i. Size: 1.13 mL

ii. Contains

a) MgCl2

b) dATP

c) dGTP

d) dCTP

e) dTTP

f) bovine serum albumin

g) enzyme

h) 0.05% sodium azide in buffer and salt.

b. GlobalFiler Express Allelic Ladder, 1 tube,

i. Size: 0.065 mL

ii. Contains the following amplified alleles:

a) 6-FAM™ dye (blue) - CODIS/ESS Markers:

1) D3S1358

2) vWA

3) D16S539

4) CSF1PO

5) TPOX

b) VIC® dye (green) - CODIS/ESS Markers:

1) Y indel 1, 2

2) Amelogenin X, Y

3) D8S1179

4) D21S11

5) D18S51

6) DYS391

c) NED™ dye (yellow) - CODIS/ESS Markers:

1) D2S441

2) D19S433

3) FGA

d) TAZ™ dye (red) - CODIS/ESS Markers:

1) D22S1045

2) D5S818

3) D13S317

4) D7S820

5) SE33

e) SID™ dye (purple) - CODIS/ESS Markers:

1) D10S1248

2) D1S1656

3) D2S1338

c. DNA Control 007, 1 tube

i. Size: 0.05 mL

ii. Contains 2 ng/μL human male genomic DNA in 0.05% sodium azide and buffer.

d. Master Mix Additive, 1 tube

i. Size: 0.1 mL

ii. Reagent for one time addition to the GlobalFiler Express Master Mix following first thaw

e. GlobalFiler Express Primer, 1 tube

i. Size: 1.2 mL

ii. Contains forward and reverse primers to amplify human DNA targets.

2. Shipping Condition: Dry Ice

3. Storage Temperature: -15°C to -25°C upon receipt and 2°C to 8°C after initial use for up to 6 months

4. Number of Reactions: 200

5. UOM: Kit

Proprietary Line 8: GlobalFiler Express PCR Amplification Kit, 1000 Reactions Proprietary Brand/Product No.: Life Technologies Corporation # 4474665

1. Each kit shall contain:

a. GlobalFiler Express Master Mix, 1 bottle

i. Size: 5.64 mL

ii. Contains

a) MgCl2

b) dATP

c) dGTP

d) dCTP

e) dTTP

f) bovine serum albumin

g) enzyme

h) 0.05% sodium azide in buffer and salt.

b. GlobalFiler Express Allelic Ladder, 1 tube

i. Size: 0.15 mL

ii. Contains the following amplified alleles:

a) 6-FAM™ dye (blue) - CODIS/ESS Markers:

1) D3S1358

2) vWA

3) D16S539

4) CSF1PO

5) TPOX

b) VIC® dye (green) - CODIS/ESS Markers:

1) Y indel 1, 2

2) Amelogenin X, Y

3) D8S1179

4) D21S11

5) D18S51

6) DYS391

c) NED™ dye (yellow) - CODIS/ESS Markers:

1) D2S441

2) D19S433

3) FGA

d) TAZ™ dye (red) - CODIS/ESS Markers:

1) D22S1045

2) D5S818

3) D13S317

4) D7S820

5) SE33

e) SID™ dye (purple) - CODIS/ESS Markers:

1) D10S1248

2) D1S1656

3) D2S1338

c. DNA Control 007, 1 tube

i. Size: 0.1 mL

ii. Contains 2 ng/μL human male genomic DNA in 0.05% sodium azide and buffer.

d. Master Mix Additive, 1 tube

i. Size: 0.45 mL

ii. Reagent for one time addition to the GlobalFiler Express Master Mix following first thaw

e. GlobalFiler Express Primer, 1 tube

i. Size: 16 mL

ii. Contains forward and reverse primers to amplify human DNA targets.

2. Shipping Condition: Dry Ice

3. Storage Temperature: -15°C to -25°C upon receipt and 2°C to 8°C after initial use for up to 6 months

4. Number of Reactions: 1,000

5. UOM: Kit

Proprietary Line 9: Identifiler Direct PCR Amplification Kit, 200 Reactions Proprietary Brand/Product No.: Life Technologies Corporation # 4467831 Note: This item is used on existing Genetic Analyzer Models: 3100 and 3500

1. Product Size: 200 reactions

2. Technique: Short Tandem Repeat (STR) Analysis

3. Polymerase: AmpliTaq Gold DNA

4. Label or Dye: NED™, FAM™, PET®, LIZ VIC™

5. Reaction Format: SuperMix or Master Mix

6. Sample Type: Blood, Whatman FTA Cards Samples, Buccal Sample, Forensic Sample

7. AmpFlSTR® Identifiler® Direct Master Mix:

a. 2 tubes, 1.25 mL/tube

b. Contains MgCl2, dATP, dGTP, dCTP, and dTTP, bovine serum albumin, enzyme, and

0.04% sodium azide in buffer and salt.

8. AmpFlSTR® Identifiler® Direct Control DNA 9947A:

a. 1 tube, 50 μL.

b. Contains 2.0 ng/μL human cell line DNA in 0.05% sodium azide and buffer. The profile of this female DNA is CSF1PO 10,12; D2S1338 19,23; D3S1358 14,15; D5S818 11, 11;

D7S820 10,11; D8S1179 13, 13; D13S317 11, 11; D16S539 11,12; D18S51 15,19;

D19S433 14,15; D21S11 30, 30; FGA 23,24; TH01 8,9.3; TPOX 8, 8; vWA 17,18.

9. AmpFlSTR® Identifiler® Direct Primer Set:

a. 2 tubes, 1.25 mL/tube.

b. Contains locus-specific 6FAM™, VIC®, NED™, and PET® dye-labeled and unlabeled primers in buffer. The primers amplify the STR loci CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, TH01, TPOX, vWA, and the gender marker, Amelogenin.

10. AmpFlSTR® Identifiler® Direct Allelic Ladder:

a. 1 tube, 50 μL

b. Contains the following amplified alleles:

i. 6-FAM™ dye (blue): D8S1179 8–19; D21S11 24, 24.2, 25–28, 28.2, 29, 29.2, 30, 30.2, 31, 31.2, 32, 32.2, 33, 33.2, 34, 34.2, 35, 35.2, 36–38; D7S820 6–15;

CSF1PO 6–15.

ii. VIC® dye (green): D3S1358 12–19; TH01 4–9, 9.3, 10, 11, 13.3; D13S317 8–15;

D16S539 5, 8–15; D2S1338 15–28.

iii. NED™ dye (yellow): D19S433 9–12, 12.2, 13, 13.2, 14, 14.2, 15, 15.2, 16, 16.2, 17, 17.2; vWA 11–24; TPOX 6–13; D18S51 7, 9, 10, 10.2, 11–13, 13.2, 14, 14.2, 15–27.

iv. PET® dye (red): Amelogenin X and Y; D5S818 7–16; FGA 17–26, 26.2, 27–30, 30.2, 31.2, 32.2, 33.2, 42.2, 43.2, 44.2, 45.2, 46.2, 47.2, 48.2, 50.2, 51.2.

11. Shipping Condition: Dry Ice

12. Storage Temperature: -15°C to -25°C upon receipt and 2°C to 8°C after initial use for up to 6 months

13. UOM: Kit

Proprietary Line 10: Investigator 24plex GO! Kit, 200 Reactions Proprietary Brand/Product No.: Qiagen, Inc. # 382426

1. Primer Mix: 2 x 1250 µl, containing primers for the following loci (markers) labeled with the corresponding dyes:

a. Fluorescence labels: 6-FAM, BTG, BTY, BTR2, BTP

b. Dye: 6-FAM; Markers: Amelogenin, TH01, D3S1358, vWA, D21S11

c. Dye: BTG; Markers: TPOX, DYS391, D1S1656, D12S391, SE33

d. Dye: BTY; Markers: D10S1248, D22S1045, D19S433, D8S1179, D2S1338

e. Dye: BTR2; Markers: D2S441, D18S51, FGA

f. Dye: BTP; QS1, D16S539, CSF1PO, D13S317, D5S818, D7S820, QS2

2. Fast Reaction Mix 2.0: 2 x 750 µl, contains: DNA Polymerase, dNTPs, MgC12, and bovine serum albumin (BSA).

3. Control DNA 9948: (5 ng/µl), 50 µl

4. Allelic ladder 24plex: 2 x 25µl

5. DNA size standard 24plex (BTO): 110 µl

6. Protocol Card: 1

7. Kit Storage:

a. Shall be shipped on dry ice

b. Stored immediately upon receipt at – 15°C to – 30° in a constant-temperature freezer

c. Primer mix and allelic ladder must be stored protected from the light.

d. DNA samples and post-PCR reagents (allelic ladder and DNA size standard) should be stored separately from the PCR reagents.

e. Once thawed, the kit may be stored at 4°C for up to 6 months

8. UOM: Kit

Proprietary Line 11: Investigator 24plex GO! Kit, 1000 Reactions Proprietary Brand/Product No.: Qiagen, Inc. # 382428 For use with Applied Biosystems 3500/3500XL Genetic Analyzers

1. Primer Mix: 10 X 1250 µl, containing primers for the following loci (markers) labeled with the corresponding dyes:

a. Fluorescence labels: 6-FAM, BTG, BTY, BTR2, BTP

b. Dye: 6-FAM; Markers: Amelogenin, TH01, D3S1358, vWA, D21S11

c. Dye: BTG; Markers: TPOX, DYS391, D1S1656, D12S391, SE33

d. Dye: BTY; Markers: D10S1248, D22S1045, D19S433, D8S1179, D2S1338

e. Dye: BTR2; Markers: D2S441, D18S51, FGA

f. Dye: BTP; QS1, D16S539, CSF1PO, D13S317, D5S818, D7S820, QS2

2. Fast Reaction Mix 2.0: 10 X 750 µl, contains: DNA Polymerase, dNTPs, MgC12, and bovine serum albumin (BSA).

3. Control DNA 9948: (5 ng/µl) 50 µl

4. Allelic ladder 24plex: 6 x 25µl

5. DNA size standard 24plex (BTO): 5 x 110 µl

6. Protocol Card: 1

7. Kit Storage:

a. Shall be shipped on dry ice

b. Stored immediately upon receipt at – 15°C to – 30°C in a constant-temperature freezer

c. Primer mix and allelic ladder must be stored protected from the light.

d. DNA samples and post-PCR reagents (allelic ladder and DNA size standard) should be stored separately from the PCR reagents.

e. Once thawed, the kit may be stored at 4°C for up to 6 months

8. UOM: Kit

Proprietary Line 12: Investigator 24plex QS Kit, 100 Reactions Proprietary Brand/Product No.: Qiagen, Inc. # 382415 For use with Applied Biosystems 3500/3500XL Genetic Analyzers

1. Primer Mix: 4 X 250 µl, containing primers for the following loci (markers) labeled with the corresponding dyes:

a. Fluorescence labels: 6-FAM, BTG, BTY, BTR2, BTO, BTP

b. Dye: 6-FAM; Markers: Amelogenin, TH01, D3S1358, vWA, D21S11

c. Dye: BTG; Markers: TPOX, DYS391, D1S1656, D12S391, SE33

d. Dye: BTY; Markers: D10S1248, D22S1045, D19S433, D8S1179, D2S1338

e. Dye: BTR2; Markers: D2S441, D18S51, FGA

f. Dye: BTP; QS1, D16S539, CSF1PO, D13S317, D5S818, D7S820, QS2

2. Fast Reaction Mix 2.0: 4 X 750 µl, contains: DNA Polymerase, dNTPs, MgC12, and bovine serum albumin (BSA).

3. Control DNA 9948: (0.1 ng/µl), 200 µl

4. Allelic ladder 24 plex: 3 x 25µl

5. DNA size standard 550 (BTO): 220 µl

6. Nuclease-free water: 4 X 1.9 µl

7. Protocol Card: 1

8. Kit Storage:

a. Shall be shipped on dry ice

b. Stored immediately upon receipt at – 15°C to – 30°C in a constant-temperature freezer

c. Once thawed, the kit may be stored at 4°C for up to 3 months

d. The kit can be thawed and re-frozen 20 times with no effect on performance

9. UOM: Kit

Proprietary Line 13: Investigator 24plex QS Kit, 400 Reactions Proprietary Brand/Product No.: Qiagen, Inc. # 382417 For use with Applied Biosystems 3500/3500XL Genetic Analyzers

1. Primer Mix 4 X 250 µl, containing primers for the following loci (markers) labeled with the corresponding dyes:

a. Fluorescence labels: 6-FAM, BTG, BTY, BTR2, BTO, BTP

b. Dye: 6-FAM; Markers: Amelogenin, TH01, D3S1358, vWA, D21S11

c. Dye: BTG; Markers: TPOX, DYS391, D1S1656, D12S391, SE33

d. Dye: BTY; Markers: D10S1248, D22S1045, D19S433, D8S1179, D2S1338

e. Dye: BTR2; Markers: D2S441, D18S51, FGA

f. Dye: BTP; QS1, D16S539, CSF1PO, D13S317, D5S818, D7S820, QS2

2. Fast Reaction Mix 2.0: 4 X 750 µl, contains: DNA Polymerase, dNTPs, MgC12, and bovine serum albumin (BSA).

3. Control DNA 9948 (0.1 ng/µl): 200 µl

4. Allelic ladder 24 plex: 3 x 25 µl

5. DNA size standard 550 (BTO): 220 µl

6. Nuclease-free water: 4 X 1.9 µl

7. Protocol Card: 1

8. Kit Storage:

a. Shall be shipped on dry ice

b. Stored immediately upon receipt at – 15°C to – 30°C in a constant-temperature freezer

c. Once thawed, the kit may be stored at 4°C for up to 3 months

d. The kit can be thawed and re-frozen 20 times with no effect on performance

9. UOM: Kit

Proprietary Line 14: Investigator Lyse&Spin Basket Kit, 250 Preps Proprietary Brand/Product No.: Qiagen, Inc. # 19598 For use in the purification of DNA using the EZ1 Advanced XL

1. Contains:

a. Qiagen Investigator Lyse&Spin Baskets: 5x50

b. Collection Tubes: 5x50

c. Quick Start Protocol

2. Number of Preps: 250

3. UOM: Kit

Proprietary Line 15: Investigator STR Go! Lysis Buffer, 200 Swab Samples Proprietary Brand/Product No.: Qiagen, Inc. # 386516 For use with Investigator STR Assays for human identity and forensic testing

1. 200 Swab Samples

2. Lysis Buffer

3. Sample Type: Swab

4. Packaging: Plastic bottle

5. UOM: Each

Proprietary Line 16: Investigator STR Go! Punch Buffer, 200 Punch Samples Proprietary Brand/Product No.: Qiagen, Inc. # 386526 For use with Investigator STR Assays for human identity and forensic testing

1. 200 Punch Samples

2. PCR Additive for 200 samples to overcome inhibition from epithelial or blood cells on paper

3. Sample Type: Punch

4. Packaging: Plastic bottle

5. UOM: Each

Proprietary Line 17: Investigator STR Go! Punch Buffer, 1000 Punch Samples Proprietary Brand/Product No.: Qiagen, Inc. # 386528 For use with Investigator STR Assays for human identity and forensic testing

1. 1000 Punch Samples

2. PCR Additive for 1000 samples to overcome inhibition from epithelial or blood cells on paper

3. Sample Type: Punch

4. Packaging: Plastic bottle

5. UOM: Each

Proprietary Line 18: QIAamp DNA Mini Kit, 50 Preps Proprietary Brand/Product No.: Qiagen, Inc. # 51304 Note: For use with Qiagen QIAcube sequencing equipment

1. Contents:

a. QIAamp mini spin columns: 50

b. Collection Tubes (2 mL): 150

c. Buffer AL: 12 mL

d. Buffer ATL: 14 mL

e. Buffer AW1 (concentrate): 19 mL

f. Buffer AW2 (concentrate): 13 mL

g. Buffer AE: 2 x 15 mL

2. Shall be able to be stored dry at room temperature for up to 1 year without showing any reduction in performance.

3. Number of Preps: 50

4. UOM: Kit

Proprietary Line 19: QIAamp DNA Mini Kit, 250 Preps Proprietary Brand/Product No.: Qiagen, Inc. # 51306 Note: For use with Qiagen QIAcube sequencing equipment

1. Contents:

a. QIAamp mini spin columns: 250

b. Collection Tubes (2 mL): 750

c. Buffer AL: 2 x 33 mL

d. Buffer ATL: 50 mL

e. Buffer AW1 (concentrate): 98 mL

f. Buffer AW2 (concentrate): 66 mL

g. Buffer AE: 128 mL

2. Shall be able to be stored dry at room temperature for up to 1 year without showing any reduction in performance.

3. Number of Preps: 250

4. UOM: Kit

Proprietary Line 20: Qiagen 8-Rod Covers Proprietary Brand/Product No.: Qiagen, Inc. # 997004 For use with QIAsymphony SP/AS Instruments

1. 8 rod cover

2. Packaging: 144 per pack

3. UOM: Pack

Proprietary Line 21: Qiagen BT6 Matrix Standard Proprietary Brand/Product No.: Qiagen, Inc. # 386224 For use with Investigator STR Assays for human identity and forensic testing

1. 800 Reactions

2. Matrix standard for 6-FAM, BTG, BTY, BTR2, BTP, and BTO

3. Packaging: Plastic bottle

4. UOM: Each

Proprietary Line 22: Qiagen Buffer AL Proprietary Brand/Product No.: Qiagen, Inc. # 19075 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Total Volume: 264 mL

2. Lysis buffer used during DNA isolation when following QIAmp and DNeasy protocols

3. Packaging: Plastic bottle

4. UOM: Each

Proprietary Line 23: Qiagen Buffer ATL Proprietary Brand/Product No.: Qiagen, Inc. # 19076 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Total Volume: 200 mL

2. Tissue lysis buffer for use in purification of nucleic acids

3. CAS-No.: 151-21-3

4. Packaging: Plastic bottle

5. UOM: Each

Proprietary Line 24: Qiagen Buffer G2 Proprietary Brand/Product No.: Qiagen, Inc. # 1014636 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Total Volume: 260 mL

2. Lysis buffer used during DNA isolation when following Genomic-Tip and EZ1 genomic

DNA procedures

3. Packaging: Plastic bottle

4. UOM: Each

Proprietary Line 25: Qiagen Buffer MTL Proprietary Brand/Product No.: Qiagen, Inc. # 19112 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Total Volume: 54 mL

2. Enables lysis of a variety of sample types and is used together with the EZ1 DNA

Investigator Kit for automated purification of DNA from forensic and biosecurity samples

3. Packaging: Plastic bottle

4. UOM: Each

Proprietary Line 26: Qiagen Collection Tubes, 2 mL Proprietary Brand/Product No.: Qiagen, Inc. # 19201 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Total Volume: 2 mL

2. Polypropylene

3. Nonsterile

4. Packaging: 1000 tubes per pack

5. UOM: Pack

Proprietary Line 27: Qiagen Control DNA 9948 Proprietary Brand/Product No.: Qiagen, Inc. # 386041 For use with Investigator STR Assays for human identity and forensic testing

1. Control DNA for creation of positive PCR samples when preparing batches of Investigator 24plex QS and Investigator 24plex GO! STR PCR: 50 µl

2. Packaging: Plastic bottle

3. UOM: Each

Proprietary Line 28: Qiagen Elution Tubes, 1.5 mL Proprietary Brand/Product No.: Qiagen, Inc. # 1050875 For use with Qiagen QIAcube

1. Volume: 1.5 mL

2. Packaging: Set of 50

3. UOM: Pack

Proprietary Line 29: Qiagen Filter-Tips, 200 µl Proprietary Brand/Product No.: Qiagen, Inc. # 990332 For use with QIAcube or QIAsymphony SP/AS instruments

1. Volume: 200 µl

2. Disposable

3. Racked

4. Packaging: 128 tips per rack, 8 racks per pack

5. UOM: Pack

Proprietary Line 30: Qiagen Filter-Tips, 1500 µl Proprietary Brand/Product No.: Qiagen, Inc. # 997024 For use with QIAsymphony SP/AS instruments

1. Volume: 1500 µl

2. Disposable

3. Racked

4. Packaging: 128 tips per rack, 8 racks per pack

5. UOM: Pack

Proprietary Line 31: Qiagen Filter-Tips, Conductive, 50 µl Proprietary Brand/Product No.: Qiagen, Inc. # 990512 For use with Qiagen QIAgility

1. Volume: 50 µl

2. Conductive

3. Carbon-impregnated

4. Packaging: 960 tips per pack

5. UOM: Pack

Proprietary Line 32: Qiagen Filter-Tips, Conductive, 200 µl Proprietary Brand/Product No.: Qiagen, Inc. # 990522 For use with Qiagen QIAgility

1. Volume: 200 µl

2. Conductive

3. Carbon-impregnated

4. Packaging: 960 tips per pack

5. UOM: Pack

Proprietary Line 33: Qiagen Filter-Tips, Wide Bore, 1000 µl Proprietary Brand/Product No.: Qiagen, Inc. # 990452 For use with QIAcube

1. Volume: 1000 µl

2. Wide bore

3. Disposable

4. Racked

5. Packaging: 128 tips per rack, 8 racks per pack

6. UOM: Pack

Proprietary Line 34: Qiagen Proteinase K, 10 mL Proprietary Brand/Product No.: Qiagen, Inc. # 19133 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Total Volume: 10 mL

2. Ready to use solution

3. Appearance: Light cream colored liquid

4. CAS-No.: 39450-07-6

5. Packaging: Plastic bottle

6. UOM: Each

Proprietary Line 35: Qiagen Reagent Bottle, 30 mL Proprietary Brand/Product No.: Qiagen, Inc. # 990392 For use with Qiagen QIAcube and QIAsymphony AS

1. Reagent bottles with lids

2. Volume: 30 mL

3. Packaging: 6 bottles with lids per pack

4. UOM: Pack

Proprietary Line 36: Qiagen Rotor Adapter Holder Proprietary Brand/Product No.: Qiagen, Inc. # 990392 For use with Qiagen QIAcube

1. Holder for 12 disposable rotor adapters

2. UOM: Each

Proprietary Line 37: Qiagen Rotor Adapter, 10x24 Proprietary Brand/Product No.: Qiagen, Inc. # 990394 For use with Qiagen QIAcube

1. Number of Preps: 240

2. Holder for 240 disposable rotor adapters and 240 Elution tubes (1.5 mL)

3. UOM: Pack

Proprietary Line 38: Qiagen Sample Prep Cartridges, 8-well Proprietary Brand/Product No.: Qiagen, Inc. # 997002 For use with QIAsymphony SP

1. 8-well sample prep cartridge

2. Packaging: 336 per pack

3. UOM: Pack

Proprietary Line 39: Qiagen S-Blocks, 2.2 mL Proprietary Brand/Product No.: Qiagen, Inc. # 19585 For use with Qiagen EZ 1 and EZ 1 Advanced XL robotic DNA extraction instruments

1. Number of Wells: 96

2. Well Plate Material: Plastic

3. Well Shape: Square

4. Non-sterile

5. Volume: 2.2 mL

6. Packaging: 24 per case

7. UOM: Case

Proprietary Line 40: Qiagen Shaker Rack Plugs Proprietary Brand/Product No.: Qiagen, Inc. # 9017854 For use with Qiagen QIAcube

1. Packaging: Set of 12

2. UOM: Pack

Proprietary Line 41: Qiagen Tape Pads Proprietary Brand/Product No.: Qiagen, Inc. # 19570 For use with Qiagen 96-well plates

1. Adhesive tape sheets for sealing Qiagen 96-well plates and 96-well blocks

2. Packaging: 125 sheets per pack

3. UOM: Pack

Proprietary Line 42: Qiagen Tubes, Graduated, Flat Base, 5 mL Proprietary Brand/Product No.: Qiagen, Inc. # 990552 For use with Qiagen QIAgility

1. Volume: 5 mL

2. Suitable for holding diluent and master mix on the instrument worktable

3. Flat-base design with a tapered internal profile for minimum dead volume

4. Screw cap included

5. Packaging: Set of 50

6. UOM: Pack

Proprietary Line 43: QIAsymphony DNA Investigator Kit, 192 Preps Proprietary Brand/Product No.: Qiagen, Inc. # 931436 Note: For use with Qiagen QIAsymphony SP Instrument

1. Contents:

a. Reagent Cartridge: 2

b. Enzyme Rack: 2

c. Piercing Lid: 2

d. Buffer ATE: 20 mL

e. Buffer AVE: 20 mL

f. Buffer ATL: 2 x 50 mL

g. Carrier RNA: 310 µg

h. Protienase K: 3 x 1.4 mL

i. Reuse Seal Set: 2

j. Trough Cover (for magnetic particles): 1

2. Shall be able to be stored dry at room temperature (15-25°C).

3. Number of Preps: 192

4. UOM: Kit

Proprietary Line 44: Tips, LiHa, Disposable, Filtered, Hanging, 50 µL Proprietary Brand/Product No.: Tecan # 30032114 Note: For use with Liquid Handling Arms/Flexible Channel Arms on all Tecan Platforms

1. Volume: 50 µL

2. Tray Type: Hanging

3. Filtered

4. Liquid Level Detection: Conductive

5. Tip Color: Black

6. Tray Color: Green

7. Packaging: 96-tips per box

8. Case Quantity: 24 boxes per case

9. UOM: Case

Proprietary Line 45: Tips, LiHa, Disposable, Filtered, Hanging, 200 µL Proprietary Brand/Product No.: Tecan # 300000629 Note: For use with Liquid Handling Arms/Flexible Channel Arms on all Tecan Platforms

1. Volume: 200 µL

2. Tray Type: Hanging

3. Filtered

4. Pure

5. Liquid Level Detection: Conductive

6. Tip Color: Black

7. Tray Color: Blue

8. Automation Compatibility: Yes

9. Packaging: 96-tips per box

10. Case Quantity: 24 boxes per case

11. UOM: Case

Proprietary Line 46: Yfiler Plus PCR Amplification Kit, 500 Reactions Proprietary Brand/Product No.: Applied Biosystems # 4482730

1. Each kit shall contain:

a. Yfiler Plus Master Mix, 4 bottles

i. Size: 1.25 mL

ii. Contains

a) MgCl2

b) dATP

c) dGTP

d) dCTP

e) dTTP

f) bovine serum albumin

g) enzyme

h) 0.05% sodium azide in buffer and salt.

b. Yfiler Plus Primer Set, 2 bottles

i. Size: 1.25 mL

ii. Contains locus-specific 6-FAM™, VIC™, NED™, TAZ™, and SID™ dye-labeled and unlabeled primers in buffer. The primers amplify:

a) the Y-STR loci DYS19

b) DYS385 a/b

c) DYF387S1 a/b

d) DYS389 I/II

e) DYS390

f) DYS391

g) DYS392

h) DYS393

i) DYS437

j) DYS438

k) DYS439

l) DYS448

m) DYS449

n) DYS456

o) DYS458

p) DYS460

q) DYS481

r) DYS518

s) DYS533

t) DYS570

u) DYS576

v) DYS627

w) DYS635 (Y GATA C4)

x) Y GATA H4

c. Yfiler Plus Allelic Ladder, 2 tubes

i. Size: 0.05 mL

ii. Contains the following amplified alleles:

a) 6-FAM™ dye (blue) - CODIS/ESS Markers:

1) DYS389I 9–17

2) DYS389II 24–35

3) DYS576 10–25

4) DYS627 11–27

5) DYS635 15–30

b) VIC® dye (green) - CODIS/ESS Markers:

1) DYS19 10–19

2) DYS391 5–16

3) DYS448 14–24

4) DYS458 11–24

5) DYS460 7–14

6) Y GATA H4 8–15

c) NED™ dye (yellow) - CODIS/ESS Markers:

1) DYS390 17–29

2) DYS392 4–20

3) DYS438 6–16

4) DYS456 10–24

5) DYS518 32–49

d) TAZ™ dye (red) - CODIS/ESS Markers:

1) DYS385 a/b 32–49

2) DYS437 10–18

3) DYS449 22–40

4) DYS570 10–26

e) SID™ dye (purple) - CODIS/ESS Markers:

1) DYF387S1 a/b 30–44

2) DYS393 7– 18

3) DYS439 6–17

4) DYS481 17–32

5) DYS533 7–17

d. DNA Control 007, 2 tubes

i. Size: 0.05 mL

ii. Contains 2 ng/μL human male genomic DNA in 0.05% sodium azide and buffer.

2. Shipping Condition: Dry Ice

3. Storage Temperature: -15°C to -25°C upon receipt and 2°C to 8°C after initial use for up to 6 months

4. Number of Reactions: 500

5. UOM: Kit

175-67 - Pipetters, Pullers and Dispensers

Proprietary Line 47: Multi-Pipette, Manual, Eight-Channel Proprietary Brand/Product No.: Mettler Toledo Rainin L8-10XLS+ # 17013802

1. Manual 8-channel pipette

2. Volume: 1 µL; 5 µL; 10 µL

3. Accuracy: ± 4 % ; 0.04 µL ; 1.5 % ; 0.075 µL ; 1 % ; 0.1 µL

4. Precision: ± 5 % ; 0.05 µL ; 1.5 % ; 0.075 µL ; 0.5 % ; 0.05 µL

5. Increment: 0.02 µL

6. UOM: Each

Proprietary Line 48: Pipet, Electronic Repeater, Positive Displacement Proprietary Brand/Product No.: Mettler Toledo Rainin NanoRep # 30568171

1. Electronic repeater pipette with non-contact dispensing

2. 10 mL syringe tip

3. Joystick operated with clear color screen

4. Lowest Aliquot: 100 nL

5. Increment: 10 nL

6. Number of Aliquots per Aspiration: up to 1,000

7. UOM: Each

Proprietary Line 49: Pipet, Manual, Single-Channel, 2 µL Proprietary Brand/Product No.: Mettler Toledo Rainin Pipet-Lite LTS L-2XLS+ # 17014393

1. Manual single-channel pipette

2. Volume: 0.2 µL; 1 µL; 2 µL

3. Accuracy ±: 12 % ; 0.024 µL ; 2.7 % ; 0.027 µL ; 1.5 % ; 0.03 µL

4. Precision ±: 6 % ; 0.012 µL ; 1.3 % ; 0.013 µL ; 0.7 % ; 0.014 µL

5. Increment: 0.02 µL

6. UOM: Each

Proprietary Line 50: Pipet, Manual, Single-Channel, 10 µL Proprietary Brand/Product No.: Mettler Toledo Rainin Pipet-Lite LTS L-10XLS+ # 17014388

1. Manual single-channel pipette

2. Volume: 1 µL; 5 µL; 10 µL

3. Accuracy ±: 2.5 % ; 0.025 µL ; 1.5 % ; 0.075 µL ; 1 % ; 0.1 µL

4. Precision ±: 1.2 % ; 0.012 µL ; 0.6 % ; 0.03 µL ; 0.4 % ; 0.04 µL

5. Increment: 0.02 µL

6. UOM: Each

Proprietary Line 51: Pipet, Manual, Single-Channel, 20 µL Proprietary Brand/Product No.: Mettler Toledo Rainin Pipet-Lite LTS L-20XLS+ # 17014392

1. Manual single-channel pipette

2. Volume: 2 µL; 10 µL; 20 µL

3. Accuracy ±: 7.5 % ; 0.15 µL ; 1.5 % ; 0.15 µL ; 1 % ; 0.2 µL

4. Precision ±: 2 % ; 0.04 µL ; 0.5 % ; 0.05 µL ; 0.3 % ; 0.06 µL

5. Increment: 0.02 µL

6. UOM: Each

Proprietary Line 52: Pipet, Manual, Single-Channel, 100 µL Proprietary Brand/Product No.: Mettler Toledo Rainin Pipet-Lite LTS L-100XLS+ # 17014384

1. Manual single-channel pipette

2. Volume: 10 µL; 50 µL; 100 µL

3. Accuracy ±: 3.5 % ; 0.35 µL ; 0.8 % ; 0.4 µL ; 0.8 % ; 0.8 µL

4. Precision ±: 1 % ; 0.1 µL ; 0.24 % ; 0.12 µL ; 0.15 % ; 0.15 µL

5. Increment: 0.02 µL

6. UOM: Each

Proprietary Line 53: Pipet, Manual, Single-Channel, 200 µL Proprietary Brand/Product No.: Mettler Toledo Rainin Pipet-Lite LTS L-200XLS+ # 17014391

1. Manual single-channel pipette

2. Volume: 20 µL; 100 µL; 200 µL

3. Accuracy ±: 2.5 % ; 0.5 µL ; 0.8 % ; 0.8 µL ; 0.8 % ; 1.6 µL

4. Precision ±: 1 % ; 0.2 µL ; 0.25 % ; 0.25 µL ; 0.15 % ; 0.3 µL

5. Increment: 0.02 µL

6. UOM: Each

Proprietary Line 54: Pipet, Manual, Single-Channel, 1000 µL Proprietary Brand/Product No.: Mettler Toledo Rainin Pipet-Lite LTS L-1000XLS+ # 17014382

1. Manual single-channel pipette

2. Volume: 100 µL; 500 µL; 1,000 µL

3. Accuracy ±: 3 % ; 3 µL ; 0.8 % ; 4 µL ; 0.8 % ; 8 µL

4. Precision ±: 0.6 % ; 0.6 µL ; 0.2 % ; 1 µL ; 0.15 % ; 1.5 µL

5. Increment: 0.02 µL

6. UOM: Each

175-68 - Plastic Ware, All Types

Proprietary Line 55: Trough, Disposable, 100mL, Transparent Proprietary Brand/Product No.: Tecan # 10613048

1. Volume: 100 mL

2. Material: Polypropylene

3. Color: Natural, transparent

4. Disposable

5. Automation compatible

6. Sterile: DNA-free, Dnase/RNase free, PCB inhibitor-free, ATP-free, Pyrogen-free, Endotoxin-free

7. Packaging: 108 troughs per box

8. UOM: Box