EPA-R5-GL2026-PCA-Final_v5.pdf

Notice of Funding Opportunity

Applications Due: Tuesday, June 30, 2026

Great Lakes National Program OƯice

Great Lakes Biology Monitoring Program: Phytoplankton and Chlorophyll-a Components

Funding Opportunity Number: EPA-R5-GL2026-PCA i

Contents

1. Basic Information

A. ExecuƟve Summary B. Key Information:

C. Key Dates:

D. Funding Details:

E. Agency Contact Information:

F. Other Funding Considerations

2. Eligibility

A. Eligible Applicants

(1) Other Eligibility Requirements

B. Cost Sharing

3. Program Description

A. Purpose, Priorities, and Activities B. Program Goals and Objectives C. Statutory Authority D. Funding Type E. Requirements for Increasing Access to Results of EPA-Funded Extramural Scientific Research

4. Application Contents and Format

A. Application Forms B. Project Narrative Description C. Applicants Using Contractors D. Release Copies of Applications E. Coalition Coverage F. Quality Assurance

5. Submission Requirements and Deadlines

A. Submission Dates and Times B. Unique Entity Identifier (UEI) and System for Award Management (SAM.gov) C. Submission Instructions D. Technical Issues with Submission E. Intergovernmental Review

6. Application Review Information

A. Responsiveness Review B. Review Criteria C. Review and Selection Process

(1) Other Evaluation Factors ii

D. Risk Review

7. Award Notices

8. Post-Award Requirements and Administration

A. Administrative and National Policy Requirements B. Reporting C. Subaward and Executive Compensation Reporting

9. Other Information

A. Additional Provisions for Applicants Appendix 1 - Budget Narrative Instructions

1. Basic Information U.S. Environmental Protection Agency

Great Lakes National Program OƯice

NoƟce of Funding Opportunity (NOFO) for the Great Lakes Biology Monitoring Program (GLBMP): Phytoplankton and Chlorophyll-a Components

A. ExecuƟve Summary

This Notice of Funding Opportunity (NOFO) solicits applications from eligible entities for grants to be awarded pursuant to the Great Lakes Restoration Initiative (GLRI) Action Plan IV. The Great Lakes Biology Monitoring Program fulfills EPA's statutory obligation specified in Clean Water Act Section 118(c)(B) to establish a Great Lakes system-wide surveillance network to monitor the water quality of the Great Lakes. The goals of the GLBMP are to (1) report on Great Lakes water quality and ecosystem condition using assessments of the lower food web (phytoplankton, chlorophyll, zooplankton, Mysis and benthic invertebrates) as indicators; (2) assess the impacts to the lower food web from invasive species including quagga mussels; and

(3) inform fisheries and habitat management. The Great Lakes National Program OƯice (GLNPO) is requesting applications for a project to complete sample collection and analyses in support of the Phytoplankton and Chlorophyll-a components of the GLBMP.

The targeted audience for this funding opportunity is eligible entities listed below capable of analyzing phytoplankton community abundance and composition and chlorophyll-a concentrations in water samples. The intended beneficiaries of the GLBMP are all those who live, recreate, or work within the Great Lakes basin.

C. Key Dates:

June 30, 2026 11:59 pm ET Application Submission Deadline

June 30, 2026 10:59 pm CT Application Submission Deadline (Local Time)

August 2026 Anticipated Notification of Selection

October 2026 Anticipated Award Notification

D. Funding Details:

It is anticipated that up to one award will be made under this announcement. The amount of funding is expected to be $3,600,000, depending on Agency funding levels, the quality of applications received, agency priorities, and other applicable considerations. Awards funded under this opportunity are expected to have an approximate 6-year project period.

B. Key Information:

Opportunity Number:

EPA-R5-GL2026-PCA

Assistance LisƟng:

66.469

Announcement Type:

Initial

Funding Available:

$3.6 million

Number of Awards:

1 Cooperative Agreement

E. Agency Contact Information:

Further information, if needed, may be obtained from the EPA contact(s) indicated below.

Technical and Administrative Contact: GLBMP-PCA NOFO Team, GLRI-RFA@epa.gov

F. Other Funding Considerations

Up to $3,600,000 may be awarded under this NOFO over approximately six years, consisting of incremental funding of about $600,000 per year, contingent on the quality of applications received, funding availability, future appropriations, satisfactory performance of work, and other applicable considerations. Funding each year is not guaranteed. The application should include an annualized budget and budget detail narrative for the project, and a detailed workplan covering each year of the project.

EPA reserves the right to partially fund applications by funding discrete portions or phases of proposed projects. If EPA decides to partially fund an application, it will do so in a manner that does not prejudice any applicants or affect the basis upon which the application was evaluated and selected for award to maintain the integrity of the competition and selection process.

Selection or award of funding under this NOFO is not a guarantee of future funding. EPA reserves the right to make additional awards under this announcement if additional funding becomes available after the original selections are made. Any additional selections for awards will be made no later than 6 months after the original selection decisions.

2. Eligibility A. Eligible Applicants Only these types of organizations may apply:

Indian Tribe.

State & Local Governments.

Institutions of Higher Education (IHEs).

Nonprofit organizations.

Consistent with Assistance Listing No. 66.469 and the EPA’s Policy for Competition of Assistance Agreements (EPA Order 5700.5A1), this funding opportunity is being limited to a subset of eligible applicants.

Qualified non-federal entities eligible to apply include non-federal governmental entities, nonprofit organizations, and institutions. This includes state agencies; any agency or instrumentality of local government; interstate agencies; federally recognized tribes and tribal organizations; colleges and universities; non-profit organizations; and other public or non-profit private agencies, institutions, and organizations. Non-profit organization means any corporation, trust, association, cooperative, or other organization which: (1) is operated primarily for scientific, educational, service, charitable, or similar purposes in the public interest; (2) is not organized primarily for profit; and (3) uses its net proceeds to maintain, improve, and/or expand its operations. Non-profit organizations described in Section 501(c)(4) of the Internal Revenue Code that engage in lobbying activities as defined in Section 3 of the Lobbying Disclosure Act of 1995 are not eligible applicants. "For profit" organizations, international organizations, federal agencies, and individuals are not eligible applicants.

(1) Other Eligibility Requirements Applications must meet the following threshold criteria to be considered eligible:

1. Applications must include activities taking place within the Great Lakes Basin.

2. Applications must address at least one of the program priorities listed in Section 3.A.

Purpose, Priorities, and Activities.

3. Applications must not request more than $3,600,000.

4. Applicants may only submit 1 application under this opportunity. Applicants that submit more than 1 application will be contacted to determine which application to evaluate.

The remaining application(s) will be deemed ineligible.

5. Applications must specify a start date of January 1, 2027, and must specify an end date for the cooperative agreement of no later than March 31, 2033, that allows for the completion of analysis and final data reporting for all samples collected from 2027 through 2031.

6. Applications must include detailed budget information in addition to the mandatory SF- 424A form. A detailed budget narrative is mandatory and must be included in the project narrative. The budget information in the application must include all proposed budget subcategories and how requested funds support each of the tasks proposed in the workplan. There must be suƯicient detail for EPA to determine the amount of funds going to each individual task.

To be eligible under this request, applicants must demonstrate how they will:

1. Collect phytoplankton and chlorophyll-a samples from all GLBMP stations listed in Table 1 for the years 2027-2031 using procedures that maintain comparability and continuity with historic sampling.

2. Generate and analyze data for phytoplankton community composition and biovolume and chlorophyll-a concentrations; perform analyses using appropriate methods to retain comparability and continuity with historical GLBMP data.

3. Complete statistical analysis, trend interpretation, and results reporting for the phytoplankton and chlorophyll-a datasets.

Section 6 has additional threshold criteria, including:

1. Applications must adhere to the page limit requirements. Any pages over the limit(s) in Section 4 will not be reviewed.

2. The submission must be received prior to the application deadline for consideration.

B. Cost Sharing Cost sharing is not a requirement to be eligible to apply for this funding opportunity. Any cost share proposed by an applicant will be considered voluntary. Applicants will not be scored or evaluated solely on the inclusion of voluntary committed cost share if they choose to propose it.

Voluntary committed cost sharing is a binding requirement of Federal award. Applicants who propose voluntary cost share must include the costs/contributions on the SF-424, SF-424A Budget Information form, and Budget Detail.

If an applicant proposes voluntary cost share the following apply:

1. A voluntary committed cost share is subject to the match provisions in the grant regulations

(2 CFR 200.306).

2. A voluntary committed cost share may only be met with eligible and allowable costs.

3. The recipient may not use other sources of federal funds to meet a voluntary cost share unless the statute authorizing the other federal funding provides that the federal funds may be used to meet a cost share requirement on a federal grant.

4. The recipient is legally obligated to meet any proposed voluntary cost share that is included in the approved budget.

3. Program Description A. Purpose, Priorities, and Activities Program Purpose and Priorities

The U.S. Environmental Protection Agency (EPA) Great Lakes National Program OƯice (GLNPO) Great Lakes Biology Monitoring Program (GLBMP) is a long-term monitoring program with the purpose of conducting annual monitoring and targeted science projects to assess ecosystem health, guide Great Lakes restoration, and protect the Great Lakes from current and future threats.

The GLBMP is designed to address the following priorities:

1. Report on Great Lakes water quality and ecosystem condition using assessments of the lower food web (plankton, chlorophyll, zooplankton, Mysis and benthic invertebrates) as indicators;

2. Assess the impacts to the lower food web from invasive species including quagga mussels;

and

3. Inform fisheries and habitat management.

Great Lakes Biology Monitoring Program activities are implemented to fulfill EPA's statutory obligation specified in Clean Water Act Section 118(c)(B) to establish a Great Lakes system-wide surveillance network to monitor the water quality of the Great Lakes; achieve commitments of the Great Lakes Restoration Initiative and support U.S. domestic actions of the Cooperative Science and Monitoring Initiative, lake-specific priorities identified in Lakewide Action and Management Plans (LAMPs), and other Great Lakes Water Quality Agreement (GLWQA) activities.

Phytoplankton and chlorophyll-a samples are collected in the open waters of the five Great Lakes and analyzed to assess spatial and temporal trends in phytoplankton communities. Table 1 describes GLBMP base monitoring program sampling station information and analytes.

Table 1. GLBMP base monitoring program phytoplankton and chlorophyll-a sampling station information. The applicant should demonstrate how they will collect and process these required samples. Additional stations and/or samples may be proposed as part of Supplemental Studies.

Station Type (see Figure 1)

Number of Stations per survey

Surveys when collected

Samples Collected at each station (see Rosette sampling Standard Operating Procedure (SOP) for details)

Plankton 72 Spring & Summer Phytoplankton: integrated depths & deep chlorophyll layer (DCL) depths, where present

Chlorophyll-a: integrated sample and multiple discreet depths

Benthic invertebrates

23 Summer Chlorophyll-a: integrated sample

Figure 1. Map of the Great Lakes Biology Monitoring Program (GLBMP) base program monitoring stations, including stations that are sampled for chlorophyll-a and plankton in spring and summer (white circles) and stations that are sampled only for chlorophyll-a and benthic invertebrates during summer (black circles).

Program Activities

Applicants must propose program activities that align with the stated priorities and goals of the Great Lakes Biology Monitoring Program and address the following program-specific objectives:

1. documentation of the status and trends of the water quality and ecological health of the open waters of the Great Lakes based on phytoplankton community and chlorophyll-a analyses;

Applicants may propose program activities that align with the stated priorities and goals of the Great Lakes Biology Monitoring Program and address the following additional program-specific objectives:

2. improved interpretation of fluoroprobe profile datasets, based on comparisons with other approaches for monitoring phytoplankton communities (e.g. microscopy, high-performance liquid chromatography (HPLC), etc.);

3. evaluation of artificial intelligence approaches to assess phytoplankton taxonomy compared to traditional microscopy methods; and

4. supplemental studies of phytoplankton for each lake coincident with the Cooperative Science and Monitoring Initiative (CSMI) five-year schedule.

Applicants are expected to support these program-specific objectives through completion of activities that should include:

Base Monitoring Program o Phytoplankton Collection, Taxonomic Analysis, and Biovolume Calculations;

o Chlorophyll-a Collection and Analysis;

o Statistical Analysis, Interpretation, and Reporting.

Supplemental Studies o Fluoroprobe Data Analysis;

o Artificial Intelligence Approaches; and o Lake-specific studies

Data Management and Quality Assurance

Results

Base Monitoring Program: Phytoplankton Collection, Taxonomic Analysis, and Biovolume Calculations

Applicants must describe their plans to collect, identify, enumerate, and determine biovolume for phytoplankton from all five Great Lakes in spring and summer, starting in spring 2027 through summer 2031 aboard the R/V Lake Guardian. Applicants should document their taxonomic expertise and their capability to hire and train taxonomists. Applicants should demonstrate how they intend to maintain comparability and continuity of sampling procedures and taxonomic analysis with historic methods and datasets.

Applicants may suggest modifications to historic sampling and/or taxonomic procedures with supporting rationale. The supporting rationale should demonstrate how the proposed procedures will still maintain continuity between historical data and the data collected under this project. Once the award has been made and prior to the analysis of any phytoplankton samples, the award recipient will need to demonstrate comparability and consistency with existing species identification procedures and the historical dataset.

Current GLBMP sampling procedures, analytical techniques, and historic taxonomic species lists for phytoplankton can be found by referencing the Standard Operating Procedures (SOPs) posted on the NOFO website. Summary-level phytoplankton taxonomy data (major groups) are available for direct download from the Great Lakes Environmental Database (GLENDA) via EPA’s Central Data Exchange (CDX) reporting site (https://cdx.epa.gov/). Applicants can request detailed historical data on phytoplankton taxonomy by emailing GLRI-RFA@epa.gov with the subject line “EPA-R5- GL2026-PCA: GLBMP Taxonomy Data”.

Base Monitoring Program: Chlorophyll-a Collection and Analysis

Applicants must describe their plans to collect chlorophyll-a samples from all five Great Lakes in spring and summer, starting in spring 2027 through summer 2031 aboard the R/V Lake Guardian.

Applicants must also describe their plans to analyze water samples for extracted chlorophyll-a on board the R/V Lake Guardian or at a land-based laboratory within a 2-week period of sample collection. Applicants should demonstrate how they intend to maintain comparability and continuity of chlorophyll-a sampling and analysis with historic data sets.

Applicants may suggest modifications to chlorophyll-a analysis with supporting rationale. The supporting rationale should demonstrate how the diƯerent analytical methods for chlorophyll-a determination will still maintain continuity between historical data and the data collected during this project. During the first field survey of the project period, applicants will be required to complete additional training and quality assurance checks under the supervision of EPA technical staƯ.

The current SOPs for chlorophyll-a analysis are posted on the NOFO website. Historical chlorophyll-a data are available for direct download from the Great Lakes Environmental Database (GLENDA) via EPA’s Central Data Exchange (CDX) reporting site (https://cdx.epa.gov/).

Base Monitoring Program: Statistical Analysis, Interpretation, and Reporting

Applicants must describe their plans for statistical analysis, trend interpretation, and results reporting for the phytoplankton and chlorophyll-a datasets. References to specific statistical methodologies and approaches should be included.

In this section, describe plans to ensure the monitoring and assessment information generated is made available to the Great Lakes community in a wide variety of formats, including presentations, technical reports including State of the Great Lakes (SOGL) reports, and peer-reviewed journals, and other products. Applicants are expected to discuss how this dissemination of information will occur. The successful applicant is expected to integrate recent and historical data to provide interpretation of changes to the biological communities.

Applicants should demonstrate how they will work with the EPA Project OƯicer and GLBMP Technical Lead to report on the status and trends of the phytoplankton community in the Great Lakes in a timely manner and support the development of technical reports.

Applicants should also discuss how their work will increase understanding of the biological health of the Great Lakes and the implications for ecosystem and fisheries management. The advancement of scientific knowledge may include the development of phytoplankton community-based indicators, application of ecosystem models, identification of stressor-state relationships, and the further education of graduate and undergraduate students in phytoplankton taxonomy and Great Lakes ecosystem science.

Supplemental Studies: Fluoroprobe Data Analysis

Applicants should describe their plans to implement targeted studies to further refine our understanding of how in situ fluoroprobe data relate to phytoplankton community data generated by other methods, including microscopy techniques. Applicants should specifically address comparisons at multiple depths within the deep chlorophyl layer where community changes occur over fine depth scales. Rationale should be provided regarding how the proposed approach will improve our use and interpretation of in situ fluoroprobe profile datasets. Applicants should describe how these analyses fit within their proposed budget, including any relevant information on sampling frequency, location, and depths within the water column for analysis.

Fluoroprobe profile data are collected at all GLBMP phytoplankton monitoring stations and are available by request via email GLRI-RFA@epa.gov.

Supplemental Studies: Artificial Intelligence Approaches

Applicants should describe their plans to pilot artificial intelligence approaches to estimate phytoplankton abundance and community composition and compare these results to phytoplankton community data generated by other methods, including microscopy techniques. The proposed work should evaluate the eƯectiveness of artificial intelligence approaches for phytoplankton enumeration and identification. Applicants should describe how these analyses fit within their proposed budget, including any relevant information on sampling frequency, location, and depths within the water column for analysis using advanced approaches with supporting rationale.

Supplemental Studies: Lake-specific Studies

Applicants should describe how they intend to carry out supplemental assessments of the phytoplankton community, biovolume, and distribution annually to address lake-specific data and information gaps. These studies should contribute to the information needed by resources managers and occur coincident with the Cooperative Science and Monitoring Initiative (CSMI) field year for each lake, per the schedule below:

CSMI Schedule

2027 Lake Huron

2028 Lake Ontario

2029 Lake Erie

2030 Lake Michigan

2031 Lake Superior

The program design for these supplemental studies should focus on the conditions occurring within each lake and address lake-specific data and information gaps. Studies may investigate how phytoplankton communities are impacted by factors such as, but not limited to:

a. the role of non-native species in large-scale changes to the Great Lakes lower food web;

b. fluctuations in the state of the biological community on various temporal scales (e.g., short-term, seasonal, interannual, long-term) and/or spatial scales (e.g., nearshore-oƯshore, lake basin scale, whole lake scale);

c. oligotrophication of oƯshore waters in Lakes Michigan, Huron, Erie (eastern basin) and Ontario, and the associated increases in water clarity;

d. eutrophication and algal blooms in Lake Erie and embayments; and

e. rates and mechanisms of invertebrate and fish predations.

Data Management and Quality Assurance

Applicants must describe their plans and capability to complete data recording, quality assurance, and generation of data submission materials for both phytoplankton and chlorophyll-a data. With large quantities of data being generated, a comprehensive and eƯicient data management system is necessary. Applicants should demonstrate their plans to manage all data generated from sample collection and analysis and submit data to EPA in electronic format. GLNPO will provide existing data management tools, which may be maintained and modified as needed by the grantee to meet these requirements.

Applicants must demonstrate how they will submit quality-assured analytical results for Base Monitoring Program surveys (spring and summer) within four months of the end of summer survey for chlorophyll analysis and 18 months of the end of summer survey for each year of phytoplankton taxonomic analysis and biovolume calculations (e.g., submit final verified data for all samples collected in August 2027 by March 2029). Once data are reviewed and approved by EPA, they will be made publicly available on the Great Lakes Environmental Database (GLENDA) via EPA’s Central Data Exchange (CDX) reporting site.

Progress updates on datasets and analysis generated as part of Supplemental Studies should be submitted on an annual basis, starting in year 2 of the grant period. Applicants should demonstrate how project elements and processes will allow field and lab data to be eƯiciently managed and interpreted year-to-year.

Applicants must demonstrate how they will develop, implement, and maintain a Quality Assurance Project Plan (QAPP) that is consistent with the requirements of the current version of the EPA QAPP Standard CIO 2105-S-02 and a Quality Management Plan (QMP) in accordance with the current version of the EPA QMP Standard CIO 2105 S-01. A QAPP is specific to individual projects and outlines the procedures and processes necessary to ensure the quality of data or information generated during the project. It details the project's objectives, the type and quality of environmental information required, and how it will be collected, analyzed, and used. A QMP describes and documents the organization’s Quality Program, including the program’s organizational structure, quality policies and procedures, criteria and areas of application, and roles, responsibilities and authorities. Applicants must discuss their plans for ensuring the continuity and consistency of phytoplankton taxonomy data and chlorophyll-a measurements through reproducible quality assurance and quality control samples. For phytoplankton taxonomy datasets specifically, cross-lab training and demonstration of identification consistency with the previous grant recipient may be required. Please see the Quality Assurance Resources for GLRI Grantees website for more information.

Results

Applicants must describe how their proposed project will achieve outputs and outcomes that support the program’s purpose, priority, goals, and objectives in their responses to the criteria in Section 6. The description must include how the proposed project will support GLRI Action Plan IV Measure of Progress 5.2.1. Annual Great Lakes monitoring conducted; interdisciplinary science projects and assessments implemented to support the GLRI and U.S. domestic actions in support of the Cooperative Science and Monitoring Initiative, lake-specific priorities identified in LAMPs and other GLWQA activities.

Outputs and outcomes specific to each project will be identified as deliverables in the negotiated workplan if the application is selected for award. Recipients will be expected to report progress toward the attainment of expected project outputs and outcomes during the project performance period.

The term “output” means an environmental activity, eƯort, and/or associated work product related to an environmental goal and objective that will be produced or provided over a period of time or by a specified date. Outputs may be quantitative or qualitative but must be measurable over the term of the grant funding period.

The expected outputs for the grants awarded under these guidelines should include but are not limited to:

a quantification of Great Lakes ecosystem health based on analysis of phytoplankton community structure and productivity and chlorophyll-a, incorporating both established laboratory methodologies and technology-based approaches;

an evaluation of the temporal trends of ecosystem health based on phytoplankton communities and chlorophyll-a in the Great Lakes;

development of Great Lakes scientists through the education of graduate and undergraduate students in phytoplankton taxonomy and Great Lakes ecosystem science;

improved interpretation of in situ fluorometry data for phytoplankton community assessments;

an evaluation of the use of artificial intelligence approaches for phytoplankton community assessments;

targeted studies for lake-by-lake assessment of phytoplankton communities coincident with the Cooperative Science and Monitoring Initiative (CSMI);

dissemination of results; and development of State of the Great Lakes indicator reports that use data generated through the phytoplankton and chlorophyll-a and components of the GLBMP.

The term “outcome” means the result, eƯect or consequence that will be achieved by carrying out an environmental activity, eƯort, and/or associated work product that is related to an environmental or programmatic goal or objective. Outcomes may be environmental, behavioral, health-related, or programmatic in nature, must be quantitative, and may not necessarily be achievable within a grant funding period.

EPA anticipates the outcomes from the projects awarded under this announcement may be an increase in the capacity to:

understand the biological health of the Great Lakes lower food web;

understand the spatial and temporal trends of phytoplankton and chlorophyll-a to assess

Great Lakes ecosystem health to fulfill EPA's statutory obligation specified in Clean Water

Act Section 118(c)(B); achieve commitments of the Great Lakes Restoration Initiative and support U.S. domestic actions of the Cooperative Science and Monitoring Initiative, lake-specific priorities identified in LAMPs and other GLWQA activities;

surveil for new aquatic non-native species within the phytoplankton community;

understand the impacts of changing nutrient availability (i.e., driven by dreissenid mussel feeding and/or nutrient load changes) on the phytoplankton community;

enhance the Great Lakes ecosystem health indicators; and increased application of technology-based approaches for assessing the phytoplankton community.

See EPA Order 5700.7A1: EPA’s Policy for Environmental Results under EPA Assistance Agreements for more information about outputs and outcomes.

B. Program Goals and Objectives The activities to be funded under this NOFO support Pillar 1: Clean Air, Land, and Water for Every American of EPA’s “Powering the Great American Comeback” Initiative.

This announcement will result in financial assistance for a monitoring project to fulfill EPA's statutory obligation specified in Clean Water Act Section 118(c)(B) to establish a Great Lakes system-wide surveillance network to monitor the water quality of the Great Lakes, and in furtherance of the Great Lakes Restoration Initiative (GLRI) and the Great Lakes Water Quality Agreement (GLWQA). Specifically, the goal of this NOFO is to solicit applications for monitoring phytoplankton and chlorophyll-a in the Great Lakes in support of the Great Lakes Biology Monitoring Program (GLBMP).

The Great Lakes Biological Monitoring program was established in 1983 to assess and investigate the ecological health of the Great Lakes. The program generates data to assess whole lake responses to stressors, such as changes to nutrient loads and invasive species, providing information to federal, state and tribal fisheries and water quality managers. Sampling is focused on the relatively homogeneous oƯshore waters of each lake. Phytoplankton and chlorophyll-a analyses have been an integral part of the program since its inception. More information on the Great Lakes Biology Monitoring Program can be found at https://www.epa.gov/great-lakes-monitoring/great-lakes-biology-monitoring-program.

Monitoring phytoplankton communities and chlorophyll-a is important because phytoplankton composition and biomass are strong indicators of water quality and ecological condition.

Phytoplankton species respond relatively quickly to changes in their environment, making them especially useful indicators of ecosystem shifts on both short and long timescales. In addition, phytoplankton form the base of the pelagic food web, and evaluating both abundance and community composition of the phytoplankton is essential to assess food availability for higher trophic levels. Thus, the analysis of phytoplankton communities is an important component of monitoring lake ecosystems and tracking changes due to stressors such as nutrient enrichment or depletion, pollution, and invasive species impacts.

The structure of the phytoplankton community and concentrations of chlorophyll-a have changed in many areas of the Great Lakes in recent decades in response to altered nutrient dynamics, invasive species, and other stressors. Ecological indicators based on phytoplankton and chlorophyll-a datasets have been a critical part of the information used to assess the results of management actions and to guide state and local fisheries and water quality managers. Tracking these conditions and documenting the changes to the environmental status of the Great Lakes is of critical importance to a broad stakeholder community that includes fisheries managers, water quality managers, and local communities that rely on the Great Lakes for drinking water, recreation and economic value.

Technology-based approaches for determining phytoplankton abundance community composition are developing rapidly, including imaging technologies paired with artificial intelligence approaches for phytoplankton identification. Evaluating these technologies and comparing outputs to results generating by the traditional microscopy approach is essential for understanding how technology-based approaches can complement and enhance monitoring eƯorts in the future.

C. Statutory Authority The Clean Water Act, Section 118(c)(7), as amended by Public Law 114-322 authorizes activities under this NOFO. The U.S. EPA has authority to award grants and cooperative agreements for planning, research, monitoring, outreach, and implementation projects in furtherance of the GLRI and the GLWQA. This NOFO is being oƯered under Assistance Listing 66.469.

D. Funding Type It is anticipated that cooperative agreements will be funded under this funding opportunity.

Cooperative agreements provide for substantial involvement between the EPA and the selected applicant(s) in the performance of the work supported. Although the EPA will negotiate precise terms and conditions relating to substantial involvement as part of the award process, the anticipated substantial federal involvement for these projects may include:

coordination of the successful applicant’s participation in surveys aboard the R/V Lake Guardian;

close monitoring of the successful applicant’s performance to verify the results proposed by the applicant;

collaboration during performance of the scope of work;

in accordance with 2 CFR 200.317 and 2 CFR 200.318, review of proposed procurement;

approving qualifications of key personnel (the EPA will not select employees or contractors employed by the award recipient); and review of reports and manuscripts prepared under the cooperative agreement.

The EPA does not have the authority to select employees or contractors employed by the recipient. The final decision on the content of reports rests with the recipient.

E. Requirements for Increasing Access to Results of EPA-Funded Extramural Scientific Research It is EPA Policy to ensure that the results of EPA-funded extramural scientific research are accessible to the public to the greatest extent feasible consistent with applicable law; policies and Orders; the Agency's mission; resource constraints; and U.S. national, homeland and economic security. This entails maximizing, at no charge, access by the public to peer-reviewed, scientific research journal publications or associated author manuscripts, and their underlying digital research data, created in whole or in part with EPA funds, while protecting personal privacy; recognizing proprietary interests, confidential business information and intellectual property rights; and avoiding significant negative impact on intellectual property rights, innovation, and U.S. competitiveness. EPA's Policy for Increasing Access to Results of EPA- Funded Extramural Scientific Research may be accessed at: Non-EPA Researcher Requirements.

Terms and conditions implementing this policy may be accessed at: Non-EPA Researcher Requirements.

Reasonable, necessary, and allocable costs for data management and public access as discussed in EPA's Policy for Increasing Access to Results of EPA-Funded Extramural Scientific Research may be included in extramural research applications and detailed in the budget justification described in Section 4 or as otherwise directed.

4. Application Contents and Format A. Application Forms The following forms and documents are required under this announcement:

Mandatory Documents:

1. Application for Federal Assistance (SF-424)

2. Budget Information for Non-Construction Programs (SF-424A)

3. EPA Key Contacts Form 5700-54

4. EPA Form 4700-4 Preaward Compliance Review Report

5. Project Narrative Attachment Form: use this to submit your Project Narrative

6. Budget Narrative Attachment Form

Optional Documents:

7. Other Attachments Form: Use this to submit other attachments, including the CVs of proposed project staƯ and letters of support, as applicable

Application Formatting Requirements: Each Narrative Proposal must be formatted for 8½” x 11” paper and should use no smaller than an 11-point Times New Roman font with 1” margins. Do not use a “double column” (a.k.a. newspaper) format. Do not include more than one application in any file, and do not zip the file or use a file extension for your files.

Applicants are advised that readability is of paramount importance and should take precedence in application format, including selecting a legible font type and size for use in the application.

B. Project Narrative Description Narrative Proposal

The narrative proposal (including the Summary Information Page, Workplan, Detailed Budget Narrative, Maps, and Charts and Figures,) must be no more than thirty single-spaced pages in length and include the items below, in the requested order. Excess pages will not be reviewed.

Maps, charts, pictures, and other figures must be included in the Narrative Proposal file. They may be included within the body of the workplan or as an appendix. In either case those items will be counted toward the page limit. Maps, charts, pictures, and other figures that are submitted as separate attachments will not be reviewed.

The Project Narrative should include:

A. Summary Information Page (may not exceed one page):

a. Project Title (60 characters): EPA reserves the right to change the project title for its administrative convenience

b. Applicant Information: Include applicant (organization) name, address, contact person, phone number, and e-mail address.

c. Proposed Funding Request: The total dollar amount requested from EPA. This amount must be within the total funding available.

d. Project Duration: Provide beginning and end dates for the project, consistent with the project period length specified in section 1.D. Funding Details.

e. Project Description (100 words): Summarize the proposed project using plain language. Include the expected outputs, outcomes, and environmental benefits resulting from the implementation of the project.

f. Project Location: Provide representative coordinates (latitude and longitude) for the location where analysis will be performed.

B. Workplan:

The workplan for each proposed project must explicitly describe how the proposed project meets the guidelines established in Sections 1-3 of this NOFO (including the threshold eligibility criteria in Section 2) and should address each of the evaluation criteria set forth in Section 6. The workplan should be organized in the order and with the headings and information requested below. Additional details and associated point values for each section of the workplan are described in NOFO Section 6B (Review Criteria) below.

a. Study Design: Define plans to annually collect phytoplankton and chlorophyll-a in all Great Lakes during spring and summer surveys aboard the R/V Lake Guardian.

Explain how the project team intends to carry out supplemental assessments of the phytoplankton community annually in alignment with the Cooperative Science and Monitoring Initiative field year. Describe how the project will maintain comparability and continuity with historic sampling procedures and/or suggest modifications with supporting rationale.

b. Collaboration: Describe the type of any collaboration/support proposed, how you will ensure that it will materialize during project performance, and what role it will play in the overall project. Any letters demonstrating evidence of collaboration and support should be attached using the Other Attachments Form.

c. Results (Outputs and Outcomes): Provide an overview of the anticipated outputs and outcomes resulting from implementation of the proposed project. The description must include how the proposed project will support GLRI Action Plan IV Measure of Progress 5.2.1. Annual Great Lakes monitoring conducted;

interdisciplinary science projects and assessments implemented to support the GLRI and U.S. domestic actions in support of the Cooperative Science and Monitoring Initiative, lake-specific priorities identified in LAMPs and other GLWQA activities.

d. Programmatic Capability and Past Performance: Include the PI’s and Institution’s history of managing agreements, including their history of meeting reporting requirements. Describe past project expenditure rates and the expertise of proposed project staƯ.

C. Budget Narrative: Provide a detailed narrative, as well as an accompanying budget table, explaining how EPA funds and any voluntary cost-shares will be used. Applicants should use whole-dollar amounts. The applicant must provide a detailed budget with estimated funding requested for each project task. A detailed budget narrative is mandatory and must be included in the workplan (see Section 2.A).

The budget information in the application should provide suƯicient detail for EPA to determine cost-eƯectiveness, reasonableness and allowability of costs. It must include all proposed budget subcategories present on the SF-424A if applicable (Personnel, Fringe, Travel, Supplies, Equipment, Contractual, Other and Indirect); the amount requested per subcategory and a detailed description of what the funds will be used for; and how requested funds support each of the tasks proposed in the workplan. Applicants requesting indirect costs may propose an indirect cost rate below their negotiated rate or below the federal de minimus rate (15%).

Applicants must provide the aggregate amount they propose to issue as subaward work, as well as an accompanying budget table for each subaward and a description (budget narrative) of the types of activities to be supported via subaward(s). Subaward budget tables must include all associated costs, including indirect costs.

Applicants are advised that readability is of paramount importance and should take precedence in application format, including selecting a legible font type and size for use in the application.

C. Applicants Using Contractors Applicants must compete contracts for services and products, including consultant contracts, and conduct cost and price analyses, to the extent required by the procurement provisions of the regulations at 2 CFR Part 200.

Do not name a procurement contractor (including a consultant) as a “partner” or otherwise in your application unless the contractor has been selected in compliance with competitive procurement requirements. If an applicant selected for award has named a specific subrecipient, contractor, or consultant in the application, it does not relieve the applicant of its obligations to comply with subaward and/or competitive procurement requirements.

The EPA will not consider the qualifications, experience, and expertise of named subrecipients and/or named contractor(s) during the application evaluation process unless the applicant provides documentation that it has complied with these requirements.

For additional guidance, applicants should review EPA’s Best Practice Guide for Procuring Services, Supplies, and Equipment Under EPA Assistance Agreements, EPA’s Subaward Policy, and EPA’s Subaward Policy Frequent Questions. The EPA expects recipients of funding to comply with competitive procurement contracting requirements in 2 CFR Parts 200 and 1500, as well as the requirements in 2 CFR Part 200.321 and 40 CFR Part 33 Subpart C.

D. Release Copies of Applications Copies of applications submitted under this NOFO may be made publicly available after selections are made.

Applicants should:

clearly indicate which portion(s) of the application are confidential, privileged, or sensitive information, or state ‘n/a’ or ‘not applicable’ if the application does not have confidential, privileged, or sensitive information;

not include trade secrets or commercial or financial information that is confidential or privileged, or sensitive information that would invade another individual’s personal privacy (e.g., an individual’s salary, personal email addresses, etc.);

be aware that if such information is included, it will be treated in accordance with 40 CFR 2.203.

E. Coalition Coverage A coalition is formed when two or more eligible applicants coordinate to submit a single application. Coalitions must identify which single eligible organization will be the recipient of the grant and which eligible organization(s) will receive subawards from the recipient (the “pass-through entity”). The pass-through entity that administers the grant and subawards will be accountable to the EPA for proper expenditure of the funds and reporting and will be the point of contact for the coalition. Subawards must be consistent with the definition of that term in 2 CFR

200.1 and comply with the EPA’s Subaward Policy.

F. Quality Assurance In accordance with 2 CFR Part 1500.12 and the Agency Quality Directives, all assistance agreements that include environmental information operations must submit a Quality Management Plan (QMP) for EPA approval. A QMP describes and documents the organization’s Quality Program, including the program’s organizational structure, quality policies and procedures, criteria and areas of application, and roles, responsibilities and authorities. It describes an organization's overall quality program and provides a framework for implementing quality assurance across all projects and operations within the organization.

The QMP documents all technical activities to be performed under the Quality Program and describes how the program will integrate quality assurance and quality control policies and procedures, and Quality Assurance Project Plans (QAPPs) into all environmental information operations. The QMP must comply with the requirements of the EPA Quality Directives.

EPA Project OƯicers will work with all selected recipients on quality assurance requirements, which will be documented in the grant Quality Assurance Terms and Conditions. Once the award is made, if a QMP is required for the grant, the applicant will develop a QMP in accordance with the current version of the EPA QMP Standard CIO 2105 S-01, and submit for EPA approval in accordance with the awarding EPA OƯice’s QMP. Selected applicants cannot begin environmental information operations until EPA approves the applicant’s QMP.

Projects falling under the assistance agreement that include environmental information operations are required to submit a QAPP consistent with the requirements of the current version of the EPA QAPP Standard CIO 2105-S-02. A QAPP is specific to individual projects and outlines the procedures and processes necessary to ensure the quality of data or information generated during the project. It details the project's objectives, the type and quality of environmental information required, and how it will be collected, analyzed, and used.

5. Submission Requirements and Deadlines

A. Submission Dates and Times June 30, 2026 11:59 pm ET Application Submission Deadline

June 30, 2026 10:59 pm CT Application Submission Deadline (Local Time)

Grants.gov creates a date and time record when it receives the application. If you submit the same application more than once, we will accept the last on-time submission.

B. Unique Entity Identifier (UEI) and System for Award Management (SAM.gov) SAM.gov You must have an active account with SAM.gov. SAM.gov will provide a UEI for your organization, which is required to apply for grants using Grants.gov. To register, go to SAM.gov EnƟty RegistraƟon and click Get Started. From the same page, you can also click on the EnƟty RegistraƟon Checklist for the information you will need to register. Make sure you are current with SAM.gov and UEI requirements before applying for the award.

SAM.gov registration can take several weeks. Begin that process today.

Grants.gov You must also have an acƟve account with Grants.gov. You can see step-by-step instrucƟons at the Grants.gov Quick Start Guide for Applicants.

Please visit How to Register to Apply for Grants for additional information.

C. Submission Instructions You must submit your application through Grants.gov. See Section 5.B. above for information on getting registered.

Important tips:

To begin the application process under this grant announcement, go to Grants.gov and click the red “Apply” button at the top of the view grant opportunity page associated with this opportunity.

See the Quick Start Guide for Applicants for instructions on how to submit.

Make sure your application passes the Grants.gov validation checks.

Do not encrypt, zip, or password protect any files.

Your application must be submitted by an oƯicial representative of your organization who is registered with Grants.gov and is authorized to sign applications for Federal financial assistance.

If you receive an error or the button is grayed out, it may be because you do not have the appropriate role to submit in your organization. Contact your organization’s EBiz point of contact or contact Grants.gov for assistance at 1-800-518-4726 or support@grants.gov.

See Grants.gov Errors for information on other Grants.gov errors.

The UEI listed on the application must be registered to the applicant organization's SAM.gov account. If not, the application may be deemed ineligible.

D. Technical Issues with Submission If applicants experience technical issues during the submission of an application that they are unable to resolve, follow these procedures before the application deadline date:

Contact Grants.gov Support Center before the application deadline date.

Document the Grants.gov ticket/case number.

Send an email with Funding Opportunity Number (FON): EPA-R5-GL2026-PCA in the subject line to GLRI-RFA@epa.gov before the application deadline time and date and must include the following:

o Grants.gov ticket/case number(s) o Description of the issue o The entire application package in PDF format.

Without this information, the EPA may not be able to consider applications submitted outside of…