A06_Specifications_for_Supplies_Illumina_03052025_1.pdf

SPECIFICATIONS

Project:

PR Number:

Item:

1 Product Features Name of Item: NextSeq 1000/2000 P1 XLEAP-SBS Reagent Kit (600 cycles) Manufacturer: Illumina, Inc Model Number: 20100981 Salient Characteristics:

- Powered by XLEAP chemistry

- Kitted for 600 cycles of sequencing

- Supports dual indexing of single reads

- Created specifically for NextSeq 1000/2000 machines

- Sequencing output of 10-540 Gb per run

- Multiple flow cell configurations that offer flexibility to support new and emerging applications

- Easy to use cartridges to streamline workflow, reduce chance for errors and minimize waste

Capabilities (if applicable): This sequencing kit is used with our Illumina NextSeq machine to perform metabarcoding on environmental samples.

Name of Item: NextSeq 1000/2000 P2 XLEAP-SBS Reagent Kit (100 cycles) Manufacturer: Illumina, Inc Model Number: 20100987 Salient Characteristics:

- Kitted for 100 cycles of sequencing

- Powered by XLEAP chemistry

- Supports dual indexing of single reads

- Created specifically for NextSeq 1000/2000 machines

- Sequencing output of 10-540 Gb per run

- Easy to use cartridges to streamline workflow, reduce chance for errors and minimize waste

Capabilities (if applicable): This sequencing kit is used with our Illumina NextSeq machine to perform GTSeq on finclip samples for population genetics.

Name of Item: Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples) Manufacturer: Illumina, Inc Model Number: 20091654 Salient Characteristics:

- Includes 96 indexes sufficient for labeling 96 samples

- Unique dual indexing without the need for multiple assays

Capabilities (if applicable): Dual index adapters that allow for unique sample identification and accurate processing with the sequencing technology

Name of Item: Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples) Manufacturer: Illumina, Inc Model Number: 20091656 Salient Characteristics:

Name of Item: Illumina DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

Model Number: 20091658 Salient Characteristics:

Name of Item: Illumina DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

Model Number: 20091660 Salient Characteristics:

Name of Item: NextSeq PhiX Control Kit

Model Number: FC-110-3002 Salient Characteristics:

- DNA control for Illumina NextSeq sequencing platform

- Reliable, adapter-ligated library derived from the PhiX genome, which offers benefits for sequencing and alignment

Capabilities (if applicable): Control for sequencing runs to contribute variation to the run

2. Delivery

Prices quoted must be FOB Destination

“F.o.b. destination” means --Free of expense to the Government delivered, on board the carrier’s conveyance, at a specified delivery point where the consignee’s facility (plant, warehouse, store, lot, or other location to which shipment can be made) is located; and

Supplies shall be delivered to the destination consignee’s wharf (if destination is a port city and supplies are for export), warehouse unloading platform, or receiving dock, at the expense of the contractor. The Government shall not be liable for any delivery, storage, demurrage, accessorial, or other charges involved before the actual delivery (or “constructive placement” as defined in carrier tariffs) of the supplies to the destination, unless such charges are caused by an act or order of the Government acting in its contractual capacity. If rail carrier is used, supplies shall be delivered to the specified unloading platform of the consignee. If motor carrier (including “piggyback”) is used, supplies shall be delivered to truck tailgate at the unloading platform of the consignee, except when the supplies delivered meet the requirements of Item 568 of the National Motor Freight Classification for “heavy or bulky freight.” When supplies meeting the requirements of the referenced Item 568 are delivered, unloading (including movement to the tailgate) shall be performed by the consignee, with assistance from the truck driver, if requested.

If the contractor uses rail carrier or freight forwarder for less than carload shipments, the contractor shall ensure that the carrier will furnish tailgate delivery when required, if transfer to truck is required to complete delivery to consignee.

Contractor responsibilities. The contractor shall -- (1)(i) Pack and mark the shipment to comply with contract specifications; or

(ii) In the absence of specifications, prepare the shipment in conformance with carrier requirements;

(2) Prepare and distribute commercial bills of lading;

(3) Deliver the shipment in good order and condition to the point of delivery specified in the contract;

(4) Be responsible for any loss of and/or damage to the goods occurring before receipt of the shipment by the consignee at the delivery point specified in the contract;

(5) Furnish a delivery schedule and designate the mode of delivering carrier; and

(6) Pay and bear all charges to the specified point of delivery.

3. Delivery Location:

Product shall be delivered to the following location:

Samantha Silverbrand Northeast Fishery Center, U.S. Fish and Wildlife PO Box 75 227 Washington Ave Lamar, PA 16848

4. Installation:

Prices quoted must include installation. All labor and parts associated with and required for installation are the sole responsibility of the contractor. No assistance or equipment will be provided by the government. Installation shall be completed according to industry standards.

5. Technical Point of Contact (POC):

Samantha Silverbrand Northeast Fishery Center, U.S. Fish and Wildlife PO Box 75 227 Washington Ave Lamar, PA 16848 570-726-4247 ext 50116

6. Delivery Instructions: N/A

No later than three days prior to delivery, please contact the Point of Contact listed above, to arrange a specific time for delivery. Unloading the product is the sole responsibility of the delivery carrier. No labor or equipment will be provided by the government to unload the supplies.

7.0 Customer Specific Delivery requirements N/A