D1 Purchase Description.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

PURCHASE DESCRIPTION

GentleMACS™ Octo Dissociator with Heaters (Brand-name or equal)

1. Statement of Need and Purpose: The Synaptic Functions Section, at the Division of

Intramural Research (DIR), within the National Institute of Neurological Disorders and

Stroke (NINDS) seeks to purchase a fully automated and standardized tissue dissociation or homogenization for the quality control of the lab samples.

2. Background Information and Objective: The National Institutes of Health (NIH) is the nation’s leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability, conducting, supporting and making medical discoveries that improve people’s health and save lives.

NINDS is one of 27 Institutes and Centers at the NIH and its mission is to to seek fundamental knowledge about the brain and nervous system and to use that knowledge to reduce the burden of neurological disease for all people.

The NINDS Division of Intramural Research (DIR) is the internal research program of the

NINDS and is among the largest neuroscience research centers in the world. It is home to more than 500 scientists who conduct leading-edge basic, translational, and clinical research in neuroscience, neurology, and neurosurgery research at NINDS laboratories in Bethesda, Maryland.

The Synaptic Functions Section focuses on mechanisms regulating axonal transport that is essential for the maintenance of synaptic function and axonal homeostasis. Using genetic mouse models, the lab is addressing several fundamental questions: (1) how mitochondrial transport is regulated to sense changes in synaptic activity, mitochondrial integrity, axon injury and pathological stress; (2) how neurons coordinate late endocytic transport and autophagy-lysosomal function to maintain cellular homeostasis and distal degradation capacity; (3) how impaired transport contributes to synaptic dysfunction and axonal pathology in neurodegenerative diseases. These studies have led to the identification of three motor adaptor and anchoring proteins (syntaphilin, Snapin, and syntabulin) that regulate axonal transport of mitochondria, endo-lysosomes, autophagosomes, and synaptic cargoes. The long-term goal of the laboratory is to decipher mechanisms (1) boosting axonal and synaptic energy metabolism and (2) enhancing autophagy-lysosomal function for efficient clearance of dysfunctional organelles and aggregated proteins that are associated with major neurodegenerative diseases. Pursuing these investigations will advance our knowledge of fundamental processes that may affect human neurological disorders.

The current projects carrying on in the lab largely depends on cell biology researches.

Cell culture is broadly used in all kinds of biochemical studies to investigate the protein levels, functions, interactions, and approved animal study protocol (ASP1303-21).

Thus, NINDS DIR Synaptic Functions Section requires a fully automated and standardized tissue dissociation or homogenization GentleMACS Octo Dissociator with

Heaters (brand name or equal) for the quality control of the lab’s primary cultures. Tissue dissociation is the essential basis for primary cell culture to yield cells with high viability and a minimum of cell debris. Hence, a standardized tissue dissociation protocol is critical for reliable and reproducible cellular analysis. Research at the Synaptic Function

Section (SFS) aims to investigate the role of mitochondrial metabolism in neurodegenerative diseases. Primary cell culture from rodent is restricted to the embryonic stage due to the technical difficulty that tissue dissociation of postnatal/adult/aged brain requires sophisticated mechanical and enzymatic treatment to successfully disaggregate the tightly connected neural cells. Therefore, a fully automated and standardized tissue dissociation is critical to improve the quality and viability of primary neurons from postnatal/adult/aged brains from rodent models of neurodegenerative diseases.

3. Generic Name of Product: Fully automated and standardized tissue dissociation or homogenization

4. Purchase Description:

Product’s brand name Manufacturer Part Number Quantity

GentleMACS Octo

Dissociator with Heaters

Miltenyi Biotec 130-096-427 1

5. Salient characteristics: The salient physical, functional, or performance characteristics that “equal” products must meet are as follows:

• Automated and standardized tissue dissociation or homogenization;

• Handel multiple samples at the same time;

• Handle different tissues or culture cells;

• Temperature is adjustable;

• Preparation in a closed and sterile system, providing a high level of user safety and minimize cross-contamination.

• FOB delivery at NINDS

• 12 months warranty

6. Quantity: One (1) GentleMACS™ Octo Dissociator with Heaters or Equal.

7. Delivery Date: Delivery is expected within 45 days after contractor receipt of order

(ARO). Delivery shall be FOB Destination and shall be delivered to NIH/NINDS Synaptic

Function Section, located at 35 Convent Dr, Bethesda MD 20892, USA between the hours of 8:00 am and 5:00 pm, Eastern Standard Time, Monday through Friday (except on Federal Holidays).