D1 PD.pdf

Purchase Description

Name of Item or Project Title:

Purchase of the CellTrio Cell Line Automation System (Brand-name or equal) with incubator, sample storage and retrieval system, microscope and cell counter, robotic handling with different configurations, a centrifuge and liquid handler that is all tied together with a software package to include an intuitive interface, scheduler, audit trails and error recovery. This is a

COVID-19 related activity.

Statement of Need and Purpose:

NCATS seeks to purchase the CellTrio Cell Line Automation System (Brand-name or equal) to support Stem Cell Translation Laboratory (SCTL) in the cell culture in an automated fashion as well as adding small molecules into the microplates and performing media changes in flasks and microplates as well as the NCATS ongoing work to support COVID-19 pandemic response.

Background Information and Objective:

The National Institutes of Health (NIH) is the nation’s leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability, conducting, supporting and making medical discoveries that improve people’s health and save lives.

The National Center for Advancing Translational Sciences (NCATS) is a part of the National

Institutes of Health (NIH), which mission it is to catalyze the generation of innovative methods and technologies that will enhance the development, testing and implementation of diagnostics and therapeutics across a wide range of human diseases and conditions.

The goal of the Stem Cell Translation Laboratory (SCTL) is to bring induced pluripotent stem cells (iPSC) technology closer to clinical applications and drug development. Through the

SCTL, NCATS provides researchers across various disciplines and organizations access to innovative protocols and resources to advance the translation of regenerative medicine applications. Through a multidisciplinary collaborative team approach, NCATS’ SCTL scientists aim to:

• Establish quality control (QC) standards to define pluripotency and differentiated cell types.

• Develop methods to assess heterogeneity and characterize iPSC-derived cells using multi-omics technologies.

• Develop standardized methods to produce mature and functional cells meeting the QC standards above.

• Discover, validate and disseminate small molecule reagents to replace expensive recombinant proteins, xenogeneic material and undefined media components in cell differentiation protocols.

The acquisition of the CellTrio Cell Line Automation System (Brand-name or equal) will support the SCTL in the cell culture in an automated fashion as well as adding small molecules into the microplates and performing media changes in flasks and microplates with a capper/decapper system that allows 50mL conical tubes to be used as well as a centrifuge to fully automate the process.

Currently, NCATS does not have any other robotic system that would be versatile enough to be able to handle all of these processes.

Scientific staff in the SCTL perform daily media changes and small molecule additions into their plates. Having a fully automated system to perform these tasks will make the processes better controlled in terms of timing of the treatment and will prevent person to person variation. Also, it would alleviate staff from performing the manually tasks daily. This system can also be used as a small screening system that would help NCATS automation group in the future should there be a need for extra screens to meet the demands of other divisions of NCATS.

Moreover, NCATS is supporting research activities to address the novel coronavirus 2019

(SARS-CoV-2) and the disease it causes (COVID 19). With the aim of accelerating translational research across all diseases, NCATS has developed research tools, technologies, expertise and collaborative networks that can quickly pivot to address urgent public health issues.

In addition, NCATS is enabling projects to cut through operational roadblocks. In order to accelerate the research, NCATS automation capabilities are being utilized. The automated Cell

Culture platform from CellTrio (Brand-name or equal) is designed to address some of the roadblocks and will be an important addition to close some gaps in Covid-19 research.

Thus, the acquisition of the CellTrio Cell Line Automation System (Brand-name or equal) with incubator, sample storage and retrieval system, microscope and cell counter, robotic handling with different configurations, a centrifuge and liquid handler that is all tied together with a software package to include an intuitive interface, scheduler, audit trails and error recovery will support the needs of the SCTL as well as the NCATS’s ongoing work to support the pandemic response by providing automated cell culture.

Generic Name of Product:

Cell Line Automation System

Purchase Description:

For reference purposes, the Cell Line Automation System that meets the need of this requirement is the CellTrio Cell Line Automation System (Part Number: 1-NA-2020-59A), as follows:

QTY CellTrio Cell Line Automation System (Part Number: 1-NA-2020-59A)

Robots

1 SCARA (Base Labware Handler)

1 > SCARA - Tall

2 > SCARA - Servo Gripper - One for T-flask, One for WP

1 > SCARA - Linear Rail

1 > Teaching Pendant - XYZ robot rail to carry ADP

1 XYZ Robot

1 > Cap/Decap Gripper

1 > Teaching Pendant

Incubator

1 Robo-I Plus (Tflask) - 128 T-175 Flask

1 > Decontamination

1 > Active Humidifier Module

1 > Gas Guard

8 > Extra Racks for T75

2 Robo-I (Plate) - 150 Microplate, 1 unit std plate, 1 unit deepwell

2 > Decontamination for One

2 > Active Humidifier Module

2 > Gas Guard

Stocker

3 Stocker Unit - One - Internal Stocker Unit. 1 T175 flask, 1 WP, 1 Deepwell

1 > Proximity Sensor Tower - One - For filtering out human errors

8 > Extra racks for T75 Extra racks for exchanging, 1x T175, 1x T75

Media Supply System

1 External Refrigerator - Automated 610L Refrigerator

2 Media Supply System - 6 peristaltic pump system

3 > Additional Peristaltic Pump System - 12 + 3 = 15 pump system

Air Displacement Pipettes

1 ADP 1-Ch Macro - Single channel - for serological (10mL or 25mL)

1 > Serological Pipette Holder - For dispensing, includes elevator to raise/lower

1 > Individual Z-axis

1 > Pressure Sensor Individual Channel

1 ADP 1-Ch Micro - Single channel - for micro pipet, 1mL (1000uL)

1 > Individual Z-axis

1 > Pressure Sensor Individual Channel

Imagers - Counters

1 Imager - Incucyte S3

1 Confluency - Celltrio Confluency Imager

1 Cytation 5

Automation Zone Modules

1 Side-loading Centrifuge

1 Centrifuge Rotor

4 Centrifuge Bucket

2 T-flask Handler - Flipper, tilter for dispensing into or out of a single flask

4 T-flask Holder

1 T-flask Detacher

1 T-flask Capper/Decapper

4 Conical Tube Holder

2 Conical Tube Vortex

1 Conical Tube Flicker

1 Biomek i7 Hybrid 96MC + Span-8 (no enclosure)

1 > Pipetting Head, MC96, 5-1200uL

20 > Biomek static plate positions ALP's

2 > 1x1 Tip Load ALP for 96MC

1 > 2D tilter - Amplius Tilting ALP

4 > Peltier heat/cool - Qinstruments

1 > Orbital Shaker

1 > Kit, Installation - i-Series Workstation

1 > ALP Liquid Waste (wash station) 96MC

1 > High-Capacity Trash - Right side kit

1 > Biomek FX Device Controller I/O Box

Miscellaneous

1 Media Drain System

1 Barcode Reader

2 Internal Waste Enclosure

2 Enclosed Waste Chute

1 In & Out Feed - 2 slots

1 > In & Out Feed - Automated Door 1 door for both

1 Dispenser based plating (Combi)

1 Conical Tube Vortex (for Combi) 250ml

1 Conical Tube Holder

1 Combi Integration - Combi Pot, Drain, Stirrer, Alcohol Dispense, Lid Handler

2 Extra cameras for system monitoring - E.g. Verify pipet tips are present/absent on ADP

1 PC & Monitor

1 Enclosure

1 System Power Connecting Modules

1 Communication Connection

Software

1 Green Button Go - Scheduling / Planning Software

Delivery, Installation, Warranty

1 Shipping and Handling FOB Origin to NCATS

1 Factory Acceptance Testing (FAT)

1 Installation on-site - Site Acceptance Testing (SAT)

1 Training to operate the system

1 12 months warranty from SAT

The salient characteristics of the brand-name or equal CellTrio Cell Line Automation

System (Part Number: 1-NA-2020-59A) include:

• Capable of processing, harvesting, and counting cells in microplates.

• Capable of adding small molecules and growth factors into microplates.

• Monitor the cells in not only flasks but also in multi well microplates.

• Centrifuge with speed 200-4500rpm

• 96 well and 8 channel pipette head that enables researchers to set up their basic research and also their small screens.

• Refrigerated plate storage system that allows researchers to store their small molecules and growth factors and use them on demand.

• Maximum Footprint must be 4861.5 x 1974 x 2117 mm.

• Handling and Shipping, FOB Origin.

• Factory Acceptance Testing (FAT)

• Installation on-site - Site Acceptance Testing (SAT)

• Training to operate the system

• 12 months warranty from SAT

Quantity:

One CellTrio Cell Line Automation System.

Delivery:

Delivery is required within eleven (11) months after receipt of order (ARO) including Factory

Acceptance Testing (FAT) and Site Acceptance Testing (SAT). Delivery shall be made to the below address with FOB Origin. Shipping and handling shall be included in the quote.

National Center for Advancing Translational Sciences (NCATS)

9800 Medical Center Drive

Rockville, MD 20850

The installation and training shall be conducted on dates and times agreed upon by the vendor and the NCATS point of contact designated on the award.