D1 PD.pdf

Purchase Description

Name of Item or Project Title:

Purchase of QX200 AutoDG Droplet Digital PCR System (ddPCR) and accessories (brand-name or equal). This is a COVID-19 related activity.

Statement of Need and Purpose:

NCATS seeks to purchase one (1) QX200 AutoDG Droplet Digital PCR System and accessories (brand-name or equal) to support ACTIV-Trace project, in its evaluation of the impact that SARS-CoV2 variants have on current vaccine and therapeutic interventions.

Background Information and Objective:

The National Institutes of Health (NIH) is the nation’s leading medical research agency and the primary Federal agency whose mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability, conducting, supporting and making medical discoveries that improve people’s health and save lives.

The National Center for Advancing Translational Sciences (NCATS) is a part of the National

Institutes of Health (NIH), which mission it is to catalyze the generation of innovative methods and technologies that will enhance the development, testing and implementation of diagnostics and therapeutics across a wide range of human diseases and conditions.

Early Translation Branch (ETB) goal is to uncover new small molecule therapeutics and advance the process of therapeutic development through a model of collaborative research.

For each collaboration, external disease experts team with the ETB’s drug discovery teams, who provide access to small molecule screening, medicinal chemistry and informatics expertise.

Thus, the acquisition of one (1) QX200 AutoDG Droplet Digital PCR System and accessories

(brand-name or equal) will support ACTIV-Trace project, in its evaluation of the impact that

SARS-CoV2 variants have on current vaccine and therapeutic interventions, as well as ongoing work for small molecule and combination screening, in silico virtual screening, nanobody/antibody design and optimization that are part of NCATS’s ongoing work to support the pandemic response.

Generic Name of Product:

Droplet Digital PCR (ddPCR) System

Purchase Description:

For reference purposes, the Droplet Digital PCR (ddPCR) System that meets the need of this requirement is the QX200 AutoDG Droplet Digital PCR System and accessories, as follows:

Item Number

Part Number

QTY Description

1 1864100 1

QX200 AutoDG Droplet Digital PCR System AutoDG Droplet Digital PCR System for EvaGreen or probe based digital PCR applications, includes Automated Droplet Generator, QX200 Droplet Reader, computer, and QuantaSoft Software

2 1814000 1 PX1 PCR Plate Sealer PCR plate sealer, includes heat sealing instrument, plate support block that holds 96-well and 384-well plates, sealing frame, power cord

3 1863004 1 ddPCR™ Droplet Reader Oil 2 L (2 x 1 L), oil for use with droplet reader in the QX200™/ QX100™ Droplet Digital™ PCR Systems

4 12001925 1 ddPCR™ 96-Well Plates Pkg of 25, clear well/clear shell semi-skirted plates, for use with QX200™/QX100™ or QX200™ AutoDG™ Droplet Digital™ PCR Systems

5 1814040 1 PCR Plate Heat Seal, foil, pierceable Pkg of 100, foil seals for PCR and QX200/QX100 Droplet Digital PCR System applications, for use with PX1 PCR Plate Sealer

6 1863024 1 ddPCR Supermix for Probes (No dUTP) 5 ml (5 x 1 ml), 2x supermix, for use in sample preparation for droplet generation in the QX200/ QX100 Droplet Digital™ PCR Systems

7 1864108 1 DG32 Automated Droplet Generator Cartridges Pkg of 30, cartridges for use with the Automated Droplet Generator in the QX200 AutoDG Droplet Digital PCR System

8 1864110 1 Automated Droplet Generation Oil for Probes 140 ml, oil for use with the Automated Droplet Generator for probe-based detection with the QX200 AutoDG Droplet Digital PCR System

9 1864125 1 Pipet Tip Waste Bins for the AutoDG System Pkg of 10, pipet tip waste bins for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

10 1851197 1 C1000 Touch™ Thermal Cycler with 96–Deep Well Reaction Module Modular thermal cycler platform, includes C1000 Touch Thermal Cycler chassis, 96--deep well reaction module, USB flash drive

11 1840138 1 384-Well Reaction Module 384-well reaction module for high throughput, fits C1000™, C1000 Touch™, and S1000™ thermal cyclers, gradient enabled

12 HSS9601 1

Hard-Shell® 96-Well PCR Plates, high profile, semi skirted, clear/clear Pkg of 25, clear shell/clear well PCR plate to fit most instruments, rigid 2-component design

13 MSC1001 1

Microseal® 'C' PCR Plate Sealing Film, adhesive, optical Pkg of 100, optically clear adhesive film for PCR plates

14 1864121 1 Pipet Tips for the AutoDG System Pkg of 40, boxes of 96 each, pipet tips for use with the Automated Droplet Generator in the QX200™ AutoDG™ Droplet Digital™ PCR System

15 n/a 1 Handling and Shipping, FOB Destination

The salient characteristics of the brand-name or equal Droplet Digital PCR (ddPCR)

System include:

• Automatic droplet generator that can partition a 20ul PCR reaction in to 20,000 nanoliter-sized droplets;

• <45 mins to complete 96 reactions;

• Accurately count target copies when multiple target copies are distributed in a droplet;

• Illuminates and detects florescence from each individual droplet;

• Performs excitation and detection of up to two fluorescent dyes per droplet. compatible with FAM, HEX (or VIC), and EvaGreen Dyes;

• Automatically detects fluorescence from both channels during the reading process;

• A linear dynamic range of 5 orders of magnitude for detection of input samples;

• Can detect one copy of target sequence from DNA samples;

• Enables the distinction between four and five copies of input target nucleic acid between samples;

• Precision of ±10%;

• Compatible with TaqMan probe chemistry;

• DNA from unread droplets can be recovered for downstream applications;

• Displays fluorescence amplitude values per droplet for FAM, HEX (or VIC), and

EvaGreen Dyes;

• Shows multiplex data for up to two targets per fluorophore, with up to two fluorophores;

• Computes concentration (copies/μl and copies/20 μl reaction) for each sample;

• Calculates copy number for target of interest using reference target for copy number variation analysis;

• Calculates fractional abundance of mutant target in wild-type background for mutation detection;

• Software can automatically set thresholds across the entire plate or for individual wells;

• Alternatively, the user can manually set thresholds;

• Software can merge results from replicate wells;

• Fraction of negative droplets for each sample is fit to a Poisson algorithm to determine absolute copy number of target DNA;

• Allows data to be exported as a .csv file (for use in Microsoft Excel or similar program);

• Allows graphs and tables to be copied to clipboard for printing from software menus;

• ddPCR Droplet Reader Oil 2 L (2 x 1 L);

• ddPCR 96-Well Plates Pkg of 25, clear well/clear shell semi-skirted plates;

• PCR Plate Heat Seal, foil, pierceable Pkg of 100, foil seals;

• ddPCR Supermix for Probes (No dUTP) 5 ml (5 x 1 ml), 2x supermix;

• Cartridges Pkg of 30;

• Oil for Probes 140 ml;

• Pipet tip waste bins Pkg of 10;

• 96-Well PCR Plates, high profile, semi skirted, clear/clear Pkg of 25;

• PCR Plate Sealing Film, adhesive, optical Pkg of 100;

• Pipet Tips Pkg of 40, boxes of 96 each; and

• Handling and Shipping, FOB Destination.

Quantity:

One (1) Droplet Digital PCR (ddPCR) System with accessories.

Delivery Date:

Delivery is required within four (4) weeks after award (ARO). Delivery shall be made to the below address with FOB Destination.

National Center for Advancing Translational Sciences (NCATS)

9800 Medical Center Drive

Rockville, MD 20850