D1 Attach 1 PD Purchase Description.pdf
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- Attached to
- P450-Glo™ CYP26A1 and CYP26B1 Screening Systems
- Solicitation number
- 75N95023Q00501-RFQ
About this file
This document is a purchase description for CRISPR edited iPSC cell lines and clonal expansion services. The collaborative team requires CRISPR edited 802-30F iPSC cell lines with specific homozygous substitutions, as well as clonal expansion of lines harboring HiBiT tags in designated loci and a knockout of the RXFP1 gene in THP1 cells. The lines must be characterized, genotypes reported, and the PAM site mutated to prevent further cutting. Delivery of one lot is required within 20 weeks of purchase placement by the Department of Health and Human Services National Institutes of Health National Institute on Drug Abuse. No options, period of performance, or pricing terms are specified in this purchase description.
The related federal contract opportunity is a solicitation from the Department of Health and Human Services for P450-GloTM CYP26A1 and CYP26B1 Screening Systems. No other details are provided about the opportunity.
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Text version
Version 11-30-2016-1 FAR Part 11—Describing Agency Needs
TEMPLATE PURCHASE DESCRIPTION
INSTRUCTIONS: This template should be used when purchase equipment, products, supplies, software, software license agreements, warranty agreements, and maintenance service agreements.
Remove the instructions from the purchase description before submitting with the purchase request.
PURCHASE DESCRIPTION
1. Statement of Need and Purpose: CRISPR edited iPSC lines needed for high-throughput screening
2. Background Information and Objective: The collaborative team aims to develop disease-relevant cell-based models to perform high-throughput screening for small molecules that rescue cellular functions.
Generic Name of Product: CRISPR edited 802-30F iPSC and clonal expansion
4. Purchase Description:
5. Salient characteristics : CRISPR edited 802-30F iPSC cell lines with N370S-GBA1 homozygous substition, L444P-GBA1 homozygous substitution, clonal expansion of line harboring HiBiT tag in the MRC1/CD206 locus, clonal expansion of line harboring a HiBiT tag in the IL12B locus, and clonal line with a knockout of RXFP1 gene in THP1 cells. The PAM site of the sequence after homologous recombination will be mutated to prevent further cutting of the DNA. Clones will be chacterized and genotype reported.
6. Quantity: 1
7. Delivery Date: 20 weeks after purchase
8. Period of Performance: n/a
9. Option(s): n/a
| TEMPLATE PURCHASE DESCRIPTION |
| INSTRUCTIONS: This template should be used when purchase equipment, products, supplies, software, software license agreements, warranty agreements, and maintenance service agreements. Remove the instructions from the purchase description before submitting with the purchase request. |
File details come from the government source that posted it. Updated .