D - Statement of Work.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

STATEMENT OF WORK (SERVICES)

(SOW)

GENERAL INFORMATION

Title of Project:

Deep Proteomic Profile of Serum Samples with Proteograph Proteomics Platform

Statement of Need and Purpose:

The purpose of this requirement is to analyze serum proteome changes in circulating factors and cellular senescence during vascular disease and aging using a large scale, unbiased analysis to measure and quantitate proteins and nanoparticles in serum samples. This will allow the Vascular Aging Biology Unit (VABU) to identify and study changes in circulating proteome expression with aging and vascular dysfunction. These analyses require accurately calibrated and well-maintained instruments for performing such large scale analyses with very precious serum samples.

Background Information and Objective:

Unbiased serum proteomics is indispensable in elucidating the intricate interplay between circulating factors and cellular senescence during vascular disease and aging. As vascular aging progresses, systemic changes in circulating proteins play a pivotal role in driving vascular dysfunction and age-related pathologies. By employing unbiased proteomic approaches, researchers can comprehensively profile the entire spectrum of proteins present in the bloodstream, uncovering novel biomarkers and pathways associated with cellular senescence.

This enables the identification of key circulating factors that mediate the crosstalk between senescent cells and vascular tissues, thereby influencing disease progression. Understanding the dynamic changes in serum proteome composition offers valuable insights into the mechanisms underlying vascular senescence, paving the way for the development of targeted interventions to combat age-related vascular diseases and promote healthy aging.

Period of Performance:

6 months after receipt of award.

SCOPE OF WORK

General Requirements:

Independently and not as an agent of the Government, the Contractor shall furnish all the necessary services, qualified personnel, material, equipment, and facilities, not otherwise provided by the Government as needed to perform the Statement of Work below:

Specific Requirements:

The contractor shall complete a full service project from serum sample to LC-MS data on the proteomic platform and analysis with key insights for 91 samples. Data analysis will include a detailed standard report after an initial 60-minute project consultation and with dedicated 10

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs hours of data analysis assistance. Analysis must be completed on the Seer Proteograph Product Suite.

LEVEL OF EFFORT:

The contractor shall provide the assay results via reporting transfer thru email.

GOVERNMENT RESPONSIBILITIES

The government will provide 91 mouse serum samples for analysis.

DELIVERY OR DELIVERABLES

• Government samples processed by Proteograph Assay o Peptides digested, desalted, quatified, and dried down for LC-MS injection

• Assay control samples desalted, quantified and dried down for LC-MS injection

• Summary report including information about samples received and peptide yields for each sample processed by Proteograph assay

REPORTING REQUIREMENTS

The vendor shall deliver the results, information analyses and/or raw mass spectrometer data to the government.

OTHER CONSIDERATIONS

Travel:

Not applicable

Information System Security Plan:

Not applicable

Data Rights:

The data produced by this project will belong to the government to be used in further data analysis and publications.