D_SOW_JHMI.pdf

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

STATEMENT OF WORK (SERVICES)

(SOW)

GENERAL INFORMATION

Title of Project:

Single Cell RNA sequencing in the support of Alzheimer’s disease related dementias research.

Statement of Need and Purpose:

The proposed scRNA-seq experiments are designed to evaluate changes in microglial composition and immune cell subsets in the brain, especially with regard to expression of inflammation-related genes, specifically NF-kB.

Background Information and Objective:

The National Institute on Aging (NIA) uses a mouse model of Parkinson’s disease in which the disease time course can be followed temporally. These experiments will be carried out in WT C57/Bl6 mice, in mice that lack specific immune cells, and in mice where the transcription factor NF-kB will be specifically manipulated in microglia. The sc libraries generated and sequenced here will provide a great deal of insight as to the function of NF-kB in dementia.

Period of Performance:

Service will be in place from 09/30/2022 to 9/30/2023.

SCOPE OF WORK

General Requirements:

The contractor shall sequence the libraries provided by the government to obtain specified sequencing reads per library pool on either the Illumina NovaSeq, NextSeq or MiSeq instruments dependent on the experimental needs. When required, quality control assessment of the library pools shall be conducted. The contractor shall then provide FASTQ files as a resultant read out of the libraries provided.

Specific Requirements:

We request sequencing of prepared libraries as pools on multiple Illumina instruments – the NovaSeq , NextSeq and MiSeq along with quality control tests. We specifically require 14 qty NovaSeq, 20 qty NextSeq, 5 qty MiSeq sequencing runs with 500 sample quality tests. We request the sequencing depth per library be adjusted per experimental needs. Once the libraries are sequenced, both the run details and the FASTQ files shall be delivered. To maintain uniformity with past experiments, that is essential for rigorous data interpretation we need to use the same facility for future studies.

Version 11-30-2016-1 FAR Part 11—Describing Agency Needs

LEVEL OF EFFORT:

N/A

GOVERNMENT RESPONSIBILITIES

The government will generate the library pools and provide them to the contractor.

DELIVERY OR DELIVERABLES

The contractor shall provide the NIA with the FASTQ files electronically and on external hard disk.

REPORTING REQUIREMENTS

N/A

OTHER CONSIDERATIONS

Travel:

N/A

Key Personnel:

N/A

Information System Security Plan:

N/A

Data Rights:

N/A Section 508—Electronic and Information Technology Standards:

N/A

Publications and Publicity:

N/A

Confidentiality of Information:

N/A